Skip to main content
SpringerLink
Log in

Vacuolar-type H+-ATPase Distribution in Unstimulated and Acetylcholine-activated Isolated Human Eccrine Sweat Glands

  • Published:
The Histochemical Journal Aims and scope Submit manuscript

Abstract

The presence and cellular distribution of subunits of the V1 sector of the vacuolar-type H+-ATPase (V-ATPase) was investigated in isolated human eccrine sweat glands. In every instance, V-ATPase was located in the cytoplasm and apical membranes of the luminal cells of the reabsorptive duct segment. In the secretory coil, both diffuse and perinuclear staining was demonstrated in the secretory cells, with additional expression at the apical and basolateral membranes and on the intercellular canaliculi. There was no detectable difference in V-ATPase expression as a result of prior application of 100 µM acetylcholine.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References cited

  • Bastani B, Purcell H, Hemken P, Trigg D, Gluck S (1991) Expression and distribution of vacuolar proton-translocating adenosine triphosphate in response to chronic acid and alkali loads in the rat. J Clin Invest 88: 126-136.

    Google Scholar 

  • Bovell DL, Elder HY, Jenkinson DM, Wilson SM (1990) The effect of removal of extracellular Cl- on the K+ (86Rb+) efflux from the isolated human sweat gland. J Physiol 420: 144P.

  • Briggman JV, Tashian RE, Spicer SS (1983) Immunocytochemical localisation of carbonic anhydrase I and II in eccrine sweat glands from control subjects and patients with cystic fibrosis. Amer J Path 112: 250-257.

    Google Scholar 

  • Brown D (1989) Membrane recycling and epithelial cell function. Am J Physiol 256: F1-F12.

    Google Scholar 

  • Brown D, Breton S (1996) Mitochondria-rich, proton-secreting epithelial cells. J Exp Biol 199: 2345-2358.

    Google Scholar 

  • Brown D, Hirsch S, Gluck S (1988a) An H+-ATPase in opposite plasma membrane domains in kidney epithelial cell subpopulations. Nature 331: 622-624.

    Google Scholar 

  • Brown D, Hirsch S, Gluck S (1988b) Localisation of a proton pumping ATPase in rat kidney. J Clin Invest 82: 2114-2126.

    Google Scholar 

  • Burry JS, Marsolais M, Laprade R, Beck JS (1997) Apical and basolateral water permeabilities of the reabsorptive duct of the human eccrine sweat gland studied in vitro. Wein Klin Wochen 109: 542.

    Google Scholar 

  • Dobson RL, Formisano V, Lobitz WC, Brophy D (1958) Some histochemical observations on the human eccrine sweat glands. J Invest Dermatol 31: 147-159.

    Google Scholar 

  • Emrich HM, Olert H (1966) pH-wert und gesamtammoniak im menschlichen Schwiss. Pflügers Arch 290: 311-314.

    Google Scholar 

  • FinbowME, Harrison MA (1997). The vacuolarHC-ATPase:Auniversal proton pump of eukaryotes. Biochem J 324: 697-712.

    Google Scholar 

  • Frizzell RA, Field M, Schultz SG (1979) Sodium-coupled transport by epithelial tissues. Am J Physiol 236: F1-F8.

    Google Scholar 

  • Hibbs RG (1958) The fine structure of human sweat glands. Am J Anat 103: 201-217.

    Google Scholar 

  • Jones CJ, Kealey T (1987) Electrophysiological and dye-coupling studies on secretory, myoepithelial and duct cells in human eccrine sweat glands, with an appendix on eccrine sweat glands isolated from patients with cystic fibrosis. J Physiol 389: 461-481.

    Google Scholar 

  • Lee CM, Jones CJ, Kealey T (1984) Biochemical and ultrastructural studies of human eccrine sweat glands isolated by shearing and maintained for seven days. J Cell Sci 72: 259-274.

    Google Scholar 

  • Montgomery I, Jenkinson DM, Elder HY, Czarnecki D, MacKie RM (1984). The effects of stimulation on the ultrastructure of the human atrichial sweat gland. 1 The fundus. Br J Derm 110: 385-397.

    Google Scholar 

  • Munger BL, Brusilow SW, Cooke RE (1961) An electron microscopic study of eccrine sweat glands in patients with cystic fibrosis of the sweat gland. J Pediatr 59: 497-511.

    Google Scholar 

  • Quinton PM (1979). Water Metabolism: Protozoa to Man. Basel: Krager.

    Google Scholar 

  • Quinton PM (1983). Chloride impermeability in cystic fibrosis. Nature 301: 421-422.

    Google Scholar 

  • Quinton PM (1999). Physiological basis of cystic fibrosis: A historical perspective. Physiol Rev 79: S3-S22.

    Google Scholar 

  • Quinton PM, Tormey JM (1976) Localisation of Na/KATPase sites in the secretory and reabsorptive epithelia of perfused eccrine sweat glands: A question to the role of the enzyme in secretion. J Membr Biol 29: 383-399.

    Google Scholar 

  • Quinton PM, Reddy MM (1989) Cl- conductance and acid secretion in the human sweat duct. Ann NY Acad Sci 574: 438-446.

    Google Scholar 

  • Quinton PM, Elder HY, Jenkinson DMcE, Bovell DL (1999) The structure and function of human sweat glands. In: Karl Laden, ed. Antiperspirants and Deodorants, 2nd edn. New York: Marcel Dekker, pp. 17-57.

    Google Scholar 

  • Reddy MM, Quinton PM (1993) HCO3 - impermeability via CFTR Cl conductance in sweat duct. Pediatr Pulmonol 9: R21.

    Google Scholar 

  • Reddy MM, Quinton PM (1994a) Rapid regulation of electrolyte absorption in sweat duct. J Membr Biol 140: 57-67.

    Google Scholar 

  • Reddy MM, Quinton PM (1994b) Intracellular Cl activity-evidence of dual mechanisms of Cl absorption in sweat duct. Am J Physiol 36(4): C1136-C1144.

    Google Scholar 

  • Reddy MM, Quinton PM (1996) Hydrolytic and non-hydrolytic interactions in the ATP regulation of CFTR Cl- conductance. Am J Physiol 270: C35-C42.

    Google Scholar 

  • Reddy MM, Light MJ, Quinton PM (1999) Activation of the epithelial NaC channel (ENaC) requires CFTR Cl-channel function. Nature 402: 301-304.

    Google Scholar 

  • Roussa E, Thevenod F (1998) Distribution of V-ATPase in rat salivary gland. Eur J Morphol 36(Suppl.): 147-152.

    Google Scholar 

  • Sato K (1973) Sweat induction from an isolated eccrine sweat gland. Am J Physiol 225(5): 1147-1152.

    Google Scholar 

  • Sato K, Kang WH, Saga K, Sato KT (1989) Biology of the sweat glands and their disorders. I. Normal sweat gland function. J Am Acad Dermatol 20: 537-563.

    Google Scholar 

  • Schultz I (1969) Micropuncture studies of the sweat formation in cystic fibrosis patients. J Clin Invest 48: 1470-1477.

    Google Scholar 

  • Silva P, Stoff J, Field M, Fine L, Forrest JN, Epstein JH (1977) Mechanism of active chloride secretion by shark rectal gland: Role of Na-K-ATPase in chloride transport. Am J Physiol 232: F298-F306.

    Google Scholar 

  • Thaysen JH (1978) The Sweat Glands. Heidelberg, New York: Springer-Verlag.

    Google Scholar 

  • Wieczorek H, Brown D, Grinstein S, Eherenfeld J, Harvey WR (1999) Animal plasma membrane energization by proton-motive V-ATPases. BioEssays 21: 637-648.

    Google Scholar 

  • Wilson SM, Bovell DL, Elder HY, Jenkinson DM, Pediani JD (1990) The effects of removing external sodium upon the control of potassium (86Rb+) permeability in the isolated human sweat gland. Exp Physiol 75: 649-656.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. School of Biological and Biomedical Sciences, Glasgow Caledonian University, Glasgow, G4 0BA, UK

    D.L. Bovell & M.T. Clunes

  2. Department of Anatomy, University of the Saarland, Homburg/Saar, Germany

    E. Roussa

  3. Unilever Research, Port Sunlight, UK

    J. Burry

  4. Institute of Biomedical and Life Sciences, University of Glasgow, Glasgow, UK

    H.Y. Elder

Authors
  1. D.L. Bovell
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. M.T. Clunes
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. E. Roussa
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. J. Burry
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. H.Y. Elder
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Bovell, D., Clunes, M., Roussa, E. et al. Vacuolar-type H+-ATPase Distribution in Unstimulated and Acetylcholine-activated Isolated Human Eccrine Sweat Glands. Histochem J 32, 409–413 (2000). https://doi.org/10.1023/A:1004087120735

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1004087120735

Keywords

Search

Navigation