Advertisement

Genetica

, Volume 108, Issue 2, pp 113–118 | Cite as

A Population Analysis of the Structure and Variability of NOR in Salmo Trutta by Ag, CMA3 and ISH

  • P. Woznicki
  • L. Sanchez
  • P. Martinez
  • B.G. Pardo
  • M. Jankun
Article

Abstract

An analysis of the variation in the number and location of rDNA genes has been carried out in two populations of brown trout (Salmo trutta) from Poland by using Ag and CMA3-staining, and rDNA in situ hybridisation. We observed an interindividual variation in arm number with NF = 100, 101, and 102. This variation was connected with the size polymorphism of the short (NOR-bearing) arm of the chromosome pair 11. The population studied showed a multichromosomal distribution of active NORs. Atypical Ag-NORs consisted of rDNA genes, as evidenced by rDNA-ISH. In addition to individuals with standard NORs, specimens with extra NORs as well as others with only one active NOR and single interphase nucleolus were observed.

Salmo trutta Ag CMA3 ISH rDNA genes NOR polymorphism 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Allendorf, F.W. & G.H. Thorgaard, 1984. Tetraploidy and the evolution of salmonid fishes, pp. 1–54 in Evolutionary Genetics of Fishes, edited by B.J. Turner. Plenum Press, New York.Google Scholar
  2. Amaro, R., M. Abuin & L. Sanchez, 1996. Chromosomal evolution in salmonids: a comparison of Atlantic salmon, brown trout, and rainbow trout R-band chromosomes. Genetica 98: 297–302.CrossRefGoogle Scholar
  3. Castro, J., S. Rodriguez, J. Arias, L. Sanchez & P. Martinez, 1994. A population analysis of Robertsonian and Ag-NOR polymorphisms in brown trout (Salmo trutta). Theor. Appl. Genet. 89: 105–111.CrossRefGoogle Scholar
  4. Castro, J., A. Vinas, L. Sanchez & P. Martinez, 1996. Characterization of an atypical NOR site polymorphism in brown trout (Salmo trutta) with Ag-and CMA3-staining, and fluorescent in situ hybridization. Cytogenet. Cell Genet. 75: 234–239.PubMedGoogle Scholar
  5. Foresti, F., L.F. Almeida-Toledo & S.A. Toledo, 1981. Polymorphic nature of nucleolus organizer regions in fishes. Cytogenet. Cell Genet. 31: 137–144.PubMedGoogle Scholar
  6. Garrido-Ramos, M.A., M. Jamilena, R. Lozano, S. Cardenas, C. Ruiz Rejon & M. Ruiz Rejon, 1995. Cytogenetic analysis of gilthead seabream Sparus aurata (Pisces, Perciformes), a deletion affecting the NOR in a hatchery stock. Cytogenet. Cell Genet. 68: 3–7.PubMedGoogle Scholar
  7. Gold, J.R., 1984. Silver-staining and heteromorphism of chromosomal nucleolus organizer regions in North American cyprinid fishes. Copeia 1: 133–139.CrossRefGoogle Scholar
  8. Hartley, S.E., 1987. The chromosomes of salmonid fishes. Biol. Rev. 62: 197–214.CrossRefGoogle Scholar
  9. Howell, W.M. & D.A. Black, 1980. Controlled silver staining of nucleolus organizer regions with a protective colloidal developer: a 1-step method. Experientia 36: 1014–1015.PubMedCrossRefGoogle Scholar
  10. Jankun, M., A. Boron, L. Kirtiklis, A. Kirchhoffer & M. Luczynski, 1998. Cytogenetic and biochemical studies on European white-fish (Coregonus lavaretus L.) from Switzerland, in Biology and Management of Coregonid Fishes, edited by R. Eckmann, A. Appenzeller & R. Rosch. Arch. Hybrobiol. Spec. Issues Adv. Limnol. 50: 363–369.Google Scholar
  11. Kerstens, H.M.J., P.J. Poddighe & A.G.H.M. Hanselaar, 1995. A novel in situ hybridization signal amplification method based on the deposition of bitinylated tyramine. J. Histochem. Cytochem. 43: 347–352.PubMedGoogle Scholar
  12. Martinez, P., A. Vinas, C. Bouza, J. Arias, R. Amaro & L. Sanchez, 1991. Cytogenetical characterization of hatchery stocks and natural populations of Sea and Brown Trout from Northwestern Spain. Heredity 66: 9–17.Google Scholar
  13. Phillips, R.B. & P.E. Ihssen, 1985. Chromosome banding in salmonid fish: nucleolar organizer regions in Salmo and Salvelinus. Can. J. Genet. Cytol. 27: 433–440.PubMedGoogle Scholar
  14. Phillips, R.B., K.D. Zajicek & F.M. Utter, 1986. Chromosome banding in salmonid fishes: nucleolar organizer regions in Oncorhynchus. Can. J. Genet. Cytol. 28: 502–510.PubMedGoogle Scholar
  15. Reed, K.M. & R.B. Phillips, 1995. Molecular cytogenetic analysis of the double-CMA3 chromosome of lake trout, Salvelinus namaycush. Cytogenet. Cell Genet. 70: 104–107.PubMedGoogle Scholar
  16. Sanchez, L., P. Martinez, A. Vinas & C. Bouza, 1990. Analysis of the structure and variability of nucleolar organizer regions of Salmo trutta by C-, Ag-, and restriction endonuclease banding. Cytogenet. Cell Genet. 54: 6–9.PubMedGoogle Scholar
  17. Sola, L., A.R. Rossi, V. Iaselli, E.M. Rasch & P.J. Monaco, 1992. Cytogenetics of bisexual/unisexual species of Poecilia. II. Analysis of heterochromatin and nucleolar organizer regions in Poecilia mexicana maxicana by C-banding and DAPI, quinacrine, chromomycin A3, and silver staining. Cytogenet. Cell Genet. 60: 229–235.PubMedGoogle Scholar
  18. Wilson, G.N., B.A. Hollar, R.J. Waterson & R.D. Schmickel, 1978. Molecular analysis of cloned human 18S ribosomal DNA segments. Proc. Natl. Acad. Sci. USA 15: 5367–5371.CrossRefGoogle Scholar
  19. Woznicki, P. & M. Jankun, 1994. Chromosome polymorphism of Atlantic salmon (Salmo salar) from the River Dzwina, Baltic Sea Basin: arm length and NOR location variation of the eighth chromosome. Can. J. Zool. 72: 364–367.CrossRefGoogle Scholar
  20. Woznicki, P., M. Jankun & M. Luczynski, 1997. Chromosome studies in brown trout (Salmo trutta m. fario) from Poland: hypothetical evolution of the 11th, 12th and 14th chromosome pairs in Salmo karyotype. Cytobios 91: 207–214.Google Scholar
  21. Woznicki, P., M. Jankun & M. Luczynski, 1998. Chromosome polymorphism in Salmo trutta morpha lacustris from Poland, Wdzydze Lake population: variation in the short arm length of the eleventh chromosome. Aquatic Sci. 60: 367–375.Google Scholar
  22. Zhuo, L., K.M. Reed & R.B. Phillips, 1995. Hypervariability of ribosomal DNA at multiple chromosomal sites in lake trout (Salvelinus namaycush). Genome 38: 487–496.PubMedGoogle Scholar

Copyright information

© Kluwer Academic Publishers 2000

Authors and Affiliations

  • P. Woznicki
    • 1
  • L. Sanchez
    • 2
  • P. Martinez
    • 2
  • B.G. Pardo
    • 2
  • M. Jankun
    • 1
  1. 1.Department of Evolutionary EcologyOlsztyn UniversityOlsztyn-KortowoPoland
  2. 2.Departamento de Biologia Fundamental, Area de GeneticaUniversidad de Santiago de CompostelaLugoSpain

Personalised recommendations