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Expression of N-acetylglucosamine Residues in Developing Rat Fundic Gland Cells

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Abstract

The development of rat fundic gland was studied by immunohistochemistry using a recently developed monoclonal antibody, HIK 1083, at both light and electron microscope levels. Antibody HIK 1083 recognized oligosaccharides with a non-reducing terminal α-linked N-acetylglucosamine (GlcNAc) residue. In the developing rat fundic gland, cells expressing α-GlcNAc residues were discernible from day 19.5 of gestation and continued to exist till adult. The distribution of the α-GlcNAc expressing cells was consistent with that described previously for cells reacting to Griffonia simplicifolia lectin (GSA-II) in all developmental stages. These cells were located at the bottom of the fundic gland when they first appeared. With the elongation and maturation of the gland, these cells moved upwards and were finally restricted in the neck region of the gland. Combining previous reports and the present electron microscopical observations, HIK 1083-positive cells in the adult rat fundic gland are mucous neck cells. The interaction between antibody HIK 1083 and GSA-II lectin was investigated. GSA-II prevented the subsequent binding of HIK 1083, while HIK 1083 did not prevent GSA-II binding to mucous neck cells. Our results suggested that α-GlcNAc residues exist in rat fundic gland from day 19.5 of gestation and continue to exist till adult. Cells expressing α-GlcNAc residues appeared as typical mucous neck cells from postnatal four weeks.

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Authors and Affiliations

  1. Department of Anatomy, Kagoshima University Faculty of Medicine, 8-35-1 Sakuragaoka, Kagoshima, 890-8520, Japan

    Dong-Hua Yang, Shinichiro Tsuyama & Fusayoshi Murata

  2. Department of Biochemistry, Kitasato University School of Medicine, 1-15-1 Kitasato, Sagamihara, Kanagawa, 228-8520, Japan

    Kyoko Hotta

  3. Department of Laboratory Medicine, Shinshu University School of Medicine, 3-1-1 Asahi, Matsumoto, Nagano, 390-0802, Japan

    Tsutomu Katsuyama

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  1. Dong-Hua Yang
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  2. Shinichiro Tsuyama
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  4. Tsutomu Katsuyama
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  5. Fusayoshi Murata
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Yang, DH., Tsuyama, S., Hotta, K. et al. Expression of N-acetylglucosamine Residues in Developing Rat Fundic Gland Cells. Histochem J 32, 187–193 (2000). https://doi.org/10.1023/A:1004051408239

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