Skip to main content
Log in

Quantitative chromosome map of a representative indica rice

  • Published:
Euphytica Aims and scope Submit manuscript

Abstract

The somatic chromosomes of standard indica diploid rice, IR 36, were squashed on glass slides and stained with Giemsa. The condensation patterns (CP) of prometaphase chromosomes were quantitatively analysed using CHIAS III software. The relative length and centromeric index (CI) were converted from CHIAS III to numerical data and calculated by EXCEL program. The ideogram based on CP of indica rice was established. There were 2 pairs of satellite chromosomes and the result was confirmed by fluorescence in situ hybridization using 45S rDNA as a probe.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Chen, B.F. & W.K. Heneen, 1995. Satellited chromosomes, nucleolus organizer regions and nucleoli of Brassica campestris L. Hereditas 112: 113–118.

    Article  Google Scholar 

  • Fukui, K., 1986. Standardization of karyotyping plant chromosomes by a newly developed chromosome image analyzing system (CHIAS). Theor Appl Genet 72: 27–32.

    Article  Google Scholar 

  • Fukui, K., 1990. Localization of rDNA genes on rice chromosomes. Rice Biotechnol Q 1: 18–19.

    Google Scholar 

  • Fukui, K. & K. Iijima, 1991. Somatic chromosome map of rice by imaging methods. Theor Appl Genet 81: 589–596.

    Article  Google Scholar 

  • Fukui, K., N. Ohmido & G.S. Khush, 1994. Variability in rDNA loci in genus Oryza detected through fluorescence in situ hybridization. Theor Appl Genet 87: 893–899.

    Article  CAS  Google Scholar 

  • Fukui, K. & Y. Mukai, 1988. Condensation pattern as a new image parameter for identification of small chromosomes in plants. Jpn J Genet 63: 359–366.

    Google Scholar 

  • Gall, J.G. & M.L. Pardue, 1969. Formation and detection of RNADNA hybrid molecules in cytological preparations. Proc Natl Acad Sci USA 63: 378–383.

    Article  PubMed  CAS  Google Scholar 

  • Guerra, M., 1993. Cytogenetics of Rutaceae. V. High chromosomal variability in Citrus species revealed by CMA/DAPI staining. Heredity 71: 234–241.

    Google Scholar 

  • Iijima, K., K. Kakeda & K. Fukui, 1991. Identification and characterization of somatic rice chromosomes by imaging methods. Theor Appl Genet 81: 597–605.

    Article  Google Scholar 

  • John, H.A., M.L. Birristiel & K.W. Jones, 1969. RNA-DNA hybrids at the cytological level. Nature 223: 582–587.

    Article  PubMed  CAS  Google Scholar 

  • Kamisugi, Y., N. Furuya, K. Iijima & K. Fukui, 1993. Computeraided automatic identification of rice chromosomes by imaging parameters. Chromosome Res 1: 189–196.

    Article  PubMed  CAS  Google Scholar 

  • Kato, S. & K. Fukui, 1998. Condensation pattern (CP) analysis of plant chromosomes by an improved chromosome image analysing system, CHIAS III. Chromosome Res 6: 473–479.

    Article  PubMed  CAS  Google Scholar 

  • Khush, G.S., R.J. Singh, S.C. Sur & A.L. Librojo, 1984. Primary trisomics of rice origin, morphology, cytology, and use in linkage mapping. Genetics 107: 141–163.

    Google Scholar 

  • Kurata, N. & T. Omura, 1978. Karyotype analysis in rice. A new method for identifying all chromosome pairs. Jpn J Genet 53: 251–255.

    Google Scholar 

  • Kuwada, Y., 1910. A cytological study of Oryza sativa L. Bot Mag Tokyo 26: 276–281.

    Google Scholar 

  • Nakayama, S. & K. Fukui, 1997. Quantitative chromosome mapping of small plant chromosomes by improved imaging on CHIAS III. Genes Genet Syst 72: 35–40.

    Article  CAS  Google Scholar 

  • Sano, Y. & R. Sano, 1990. Variation of the intergeneric spacer region of ribosomal DNA in cultivated and wild rice species. Genome 33: 209–218.

    CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Apisitwanich, S., Shishido, R., Akiyama, Y. et al. Quantitative chromosome map of a representative indica rice. Euphytica 118, 113–118 (2001). https://doi.org/10.1023/A:1004043503389

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1004043503389

Navigation