A rapid, seedling-based bioassay for identifying low cadmium-accumulating individuals of Durum wheat (Triticum turgidum L.).


We have developed a seedling-based bioassay that iscapable of identifying low Cd-accumulating phenotypes(homozygous and heterozygous) after 96–120 h ofexposure. Our experiments were conducted using109Cd as a tracer at subtoxic concentrations tosimulate conditions that might be experienced in thefield. Supply of Cd (10-11 M) to 4-d oldseedlings for 0–8 h resulted in no differences in rootand leaf Cd content between the low (TL05) and high(TL04) Cd-accumulating isolines. Increasing time ofexposure produced significant differences in leaf Cdaccumulation between isolines, with differencesbecoming most pronounced after the bulk of appliedcadmium (∼ 95%) was depleted from absorption solutions(≥ 72 h). Similar results were obtained with8-d old seedlings, where differences between genotypeswere more pronounced in young leaves (2nd leaf) orshoot bases. Individuals from five low and highCd-accumulating near isogenic pairs (50individuals/isoline) were screened using Cdconcentration of shoot bases as the screeningcriterion. Mean scores within each isoline pair weresignificantly different, although overlap ofindividual scores was observed at intermediate foliarCd concentrations. The 2nd leaf to root Cdcontent ratio, which reflects root to shoottranslocation, provided a better parameter todistinguish low from high Cd-accumulating isolines. Plants used for this bioassay could also be rescuedfor subsequent experimental crosses, providing a rapidand cost-effective tool for early detection of the lowCd-accumulating phenotype.

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  1. Chaney, R.L., 1980. Health risks associated with toxic metals in municipal sludge. In: G. Bitton., B.L. Damron., G.T. Edds. & J.M. Davidson (Eds.), Sludge-Health Risks of Land Application. pp. 59–83.

  2. Clarke, J.M., D. Leisle, R.M. DePauw & L.L. Thiessen, 1997a. Registration of five pairs of Durum wheat genetic stocks near-isogenic for cadmium concentration. Crop Sci 37: 297–297.

    Google Scholar 

  3. Clarke J.M, D. Leisle & G.L. Kopytko, 1997b. Inheritance of cadmium concentration in five Durum wheat crosses. Crop Sci 37: 1722–1726.

    Google Scholar 

  4. Penner, G.A., J. Clarke, L.J. Bezte & D. Leisle, 1995. Identification of RAPD markers linked to a gene governing cadmium uptake in durum wheat. Genome 38: 543–547.

    Google Scholar 

  5. Ryan, J.A., H.R. Pahren & J.B. Lucas, 1982. Controlling cadmium in the human food chain. A review and rationale based on health effects. Environ Res 27: 251–302.

    Google Scholar 

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Archambault, D.J., Marentes, E., Buckley, W. et al. A rapid, seedling-based bioassay for identifying low cadmium-accumulating individuals of Durum wheat (Triticum turgidum L.).. Euphytica 117, 175–182 (2001). https://doi.org/10.1023/A:1004037901460

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  • accumulation
  • bioassay
  • cadmium
  • Durum wheat
  • grain