Abstract
Protoplasts from cell suspension cultures of ‘Bonnaza’ navel orange (Citrus sinensis (L.) Osbeck) were electrically fused with mesophyll protoplasts isolated from seedless ‘Red Blush’ grapefruit (Citrusparadisi). After 6 months of culture, a total of 20 plants were regenerated. Root tip chromosome counting revealed that 4 of them were tetraploids (2n = 4x = 36)and the rest were diploids (2n = 2x = 18) morphologically resembling the mesophyll parent. After 6 months of transplantation into the greenhouse, 4 of the diploidmesophyll regenerants unexpectedly flowered, but this phenomenon disappeared in the next year. This is the first report of precocious flowering in citrus via protoplast fusion. RAPD analysis further confirmed that the tetraploid regenerants were somatic hybrids while the diploid regenerants were mesophyll parent type. This somatic hybrid will be utilized as a possible pollen parent for improving the seedy pummelo cultivars in China by producing triploid seedless pummelo hybrid. The mechanism of early flowering was also discussed.
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Deng, X.X., J.W. Grosser & F.G. Gmitter Jr, 1992. Intergeneric somatic hybrid plants from protoplast fusion of Fortunella crassifolia cultivar 'Meiwa' with Citrus sinensis cultivar 'Valencia'. Scientia Horticulturae 49: 55-62.
Deng, X.X., W.W. Guo & G.H. Yu, 2000. Regeneration of diploid mesophyll parent type plants from nine symmetrical fusion combinations of Citrus (in Chinese). Acta Horticulturae Sinica 27: 1-5.
Gleddie, S., W.A. Keller & Setterfield, 1986. Production and characterization of somatic hybrids between Solanum melongena and S. sisymbriifolium Lam. Theor Appl Genet 71: 613-621.
Gmitter, F.G. Jr & X.B. Ling, 1991. Embryogenesis in vitro and nonchimeric tetraploid plant recovery from undeveloped citrus ovules treated with colchicine. J Amer Soc Hort Sci 116(2): 317-321.
Gmitter, F.G. Jr, X.B. Ling, C.Y. Cai & J.W. Grosser, 1991. Colchicine-induced polyploidy in citrus embryogenic cultures, somatic embryos, and regenerated plantlets. Plant Sci 74: 135-141.
Grosser, J.W. & F.G. Gmitter Jr, 1990. Protoplast fusion and citrus improvement. Plant Breed Rev 8: 339-374.
Grosser, J.W., F.A.A. Mourao-Fo, F.G. Gmitter Jr, E.S. Louzada, J. Jiang, K Baergen, A. Ouiros, C. Cabasson, J.L. Schell & J.L. Chandler, 1996a. Allotetraploid hybrids between Citrus and seven related genera produced by somatic hybridization. Theor Appl Genet 92: 577-582.
Grosser, J.W., F.G. Gmitter Jr, N. Tusa, G.R. Recupero & P. Cucinotta, 1996b. Further evidence of a cybridization requirement for plant regeneration from citrus leaf protoplasts following somatic fusion. Plant Cell Rep 15: 672-676.
Grosser, J.W., F.G. Gmitter Jr, W.S. Castle & L.J. Chandler, 1996c. Production and evaluation of citrus somatic hybrid rootstocks: progress report. In: B. Manicom et al. (Eds.), Proc. Int. Soc. Citricult, pp. 1246-1250. South Africa.
Guo, W.W. 1998. Protoplast electrofusion and genetic variation of the regenerants in Citrus (in Chinese). Ph D thesis, Huazhong Agricultural University, China.
Guo, W.W. & X.X. Deng, 2000. Two interspecific somatic hybrid plants regenerated via protoplast electro-fusion in Citrus (in Chinese). Chinese J Biotechnology 16(2): 179-182.
Guo, W.W., X.X. Deng & Y.Z. Shi, 1998. Optimization of protoplast electrofusion parameters and interspecific somatic hybrid regeneration in Citrus (in Chinese). Acta Botanica Sinica 40: 417-424.
Guo, W.W. & X.X. Deng, 1998. Somatic hybrid plantlets regeneration between Citrus and its wild relative, Murraya paniculata via protoplast electrofusion. Plant Cell Rep 18: 297-300.
Guo, W.W. & X.X. Deng, 1999. Intertribal hexaploid hybrid plants regeneration from electrofusion between diploids of Citrus sinensis and its sexually incompatible relative, Clausena lansium. Theor Appl Genet 98: 581-585.
Guo, W.W., X.X. Deng & C.G. Hu, 1999. Somatic hybrid plant regeneration between navel orange and lemon via protoplast electrofusion (in Chinese). J Wuhan Bot Res 17: 127-129.
Guo, W.W., C.H. Zhou, H.L. Yi & X.X. Deng, 2000. Intergeneric somatic hybrid plants between Citrus and Poncirus trifoliata and evaluation of their root rot resistance (in Chinese). Acta Botanica Sinica 42: 668-672.
Jumin, H.S. & N. Nito, 1995. Embryogenic protoplast cultures of orange jessamine (Murraya paniculata) and their regeneration into plants flowering in vitro. Plant Cell, Tissue and Organ Culture 41: 277-279.
Kobayashi, S., T. Ohgawara, W. Saito, Y. Nakamura & J. Shimizu, 1995. Fruit characteristics and pollen fertility of citrus somatic hybrids. J Japan Soc Hort Sci 64(2): 283-289.
Kumar, A. & E.C. Cocking, 1987. Protoplast fusion: a novel approach to orgenelle genetics in higher plants. Amer J Bot 74(8): 1289-1303.
Lee, L.S., 1988. Citrus polyploidy-origins and potential for cultivar improvement. Aust J Agric Res 39: 735-747.
Liu, J.H. & X.X. Deng, 2000. Preliminary analysis of cytoplasmic genome of diploid somatic hybrid derived from fusion between rough lemon and Hamlin sweet orange. Acta Botanica Sinica 42: 102-104.
Moriguchi, T., T. Hidaka, M. Omura, T. Motomura & T. Akihama, 1996. Genotype and parental combination influence efficiency of cybrid induction in Citrus by electrofusion. HortScience 31(2): 275-278.
Ochatt, S.J., P.K. Chand, E.L. Rech, M.R. Davey & J.B. Power, 1988. Electroporation-mediated improvement of plant regeneration from colt cherry (Prunus avium × pseudocerasus) protoplasts. Plant Sci 54: 165-169.
Ohgawara, T., S. Kobayashi, H. Uchimiya & S. Ishii, 1985. Somatic hybrid plants obtained by protoplast fusion between Citrus sinensis and Poncirus trifoliata. Theor Appl Genet 1: 1-4.
Ohgawara, T., S. Kobayashi, S. Ishii, K. Yoshinaga & I. Oiyama, 1989. Somatic hybridization in citrus: navel orange (C. sinensis Osb) and grapefruit (C. paradisi Macf). Theor Appl Genet 78: 609-612.
Saito, W., T. Ohgawara, J. Shimizu, S. Ishii & S. Kobayashi, 1993. Citrus cybrid regeneration following cell fusion between nucellar cells and mesophyll cells. Plant Sci 88: 195-201.
Saito, W., T. Ohgawara, J. Shimizu & S. Kobayashi, 1994. Somatic hybridization in Citrus using embryogenic cybrid callus. Plant Sci 99: 89-95.
Shi, Y.Z., W.W. Guo & X.X. Deng, 1998. The establishment of RAPD techniques and identification of somatic hybrids in Citrus (in Chinese). Acta Horticulturae Sinica 25(2): 105-110.
Sihachakr, D., R. Haicour, I. Serraf, E. Barrientos, C. Herbreteau, G. Ducreux, L. Rossignol & V. Souvarmavong, 1988. Electrofusion for the production of somatic hybrid plants of Solanum melongena L. and S. khasianum CB clark. Plant Sci 57: 215-223.
Soost, R.K. & J.W. Cameron, 1985. Melogold, a triploid pummelograpefruit hybrid. HortScience 20: 1134-1135.
Soost, R.K. & J.W. Cameron, 1980. Oroblanco, a triploid pummelograpefruit hybrid. HortScience 15: 667-669.
Spiegel-Roy, P. & E.E. Goldschmidt, 1996. Biology of Citrus. Cambridge University Press.
Toriyama, K., K. Hinata & T. Kameya, 1987. Production of somatic hybrid plants, 'Brassicomoricandia', through protoplast fusion between Moricandia arvensis and Brassica oleracea. Plant Sci 48: 123-128.
Yamamoto, M. & S. Kobayashi, 1995. A cybrid plant produced by electrofusion between Citrus unshiu (satsuma mandarin) and C. sinensis (sweet orange). Plant Tissue Culture Letters 12(2): 131-137.
Yamamoto, M., R. Matsumoto, N. Okudai & Y. Yamada, 1997. Aborted anthers of Citrus result from gene-cytoplasmic male sterility. Scientia Horticulturae 70: 9-14.
Ye, X.R., W.C. Zhang & S.Y. Wan, 1994. The establishment of embryogenic cell suspension cultures in Citrus (in Chinese). J Fruit Sci 11(2): 81-83.
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Guo, W., Deng, X. & Yi, H. Somatic hybrids between navel orange (Citrus sinensis) and grapefruit (C. paradisi) for seedless triploid breeding. Euphytica 116, 281–285 (2000). https://doi.org/10.1023/A:1004031621777
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DOI: https://doi.org/10.1023/A:1004031621777