Abstract
Twenty-eight species belonging to five genera of the sub tribe Cajaninae; viz., Cajanus(15 species), Rhynchosia (10 species), Dunbaria, Flemingia and Paracalyx were analysed for variations in four chloroplast generegions, rbcL, trnS-psbC, 16S and trnL-UAA. The four chloroplast gene regions were amplified with specific primers and subsequently digested with 15 restriction enzymes. Rhynchosia did not show any inter-specific differences in the four gene regions for any of the enzymes used. In Cajanus, the inter-specific PCR-RFLP profile of the four gene regions for all the enzymes were similar except for the Pst I digests of trnS-psbC gene region. At inter-generic level, rbcL gene region did not show polymorphism with any of the enzymes used; while in trnS-psbC gene region polymorphism was observed only with Pst I and Hae III digestions. Inter-generic PCR-RFLP of 16S did not reveal any variation. The trnL-UAA gene region had restriction site only forEcoR I in which Cajanus and Rhynchosia showed similar profiles while Dunbaria showed a different profile. The trnL-UAA gene region in Paracalyx and Flemingia did not have restriction site for this enzyme. Despite the analyses of four gene regions using 15 restriction enzymes, differentiation at genus level could not be obtained. These observations indicated limited divergence of the chloroplast genome among the four genera of the sub-tribe Cajaninae suggesting close relationships of the taxa.
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References
De, D.N., 1974. Pigeonpea. In: J. Hutchinson (Ed.), Evolutionary Studies in World Crops: Diversity and Change in the Indian Sub-Continent, pp. 79-87. Cambridge University Press, London.
Gamble, J.S., 1935. Flora of Presidency of Madras, 1: 277-279.
Kumar, L.S.S, M.V. Thombre & R. D'Cruz, 1958. Cytological studies of an intergeneric hybrid of Cajanus cajan (Linn.) Millsp. and Atylosia lineata W.& A. Proc. Indian. Acad. Sci. Sect. B, 47: 252-262.
Lackey, J.A., 1981. Tribe Phaseoleae. In: R.M. Polhill and P.H. Raven (Eds.), Advances in legume systematics, pp. 302-328.
Lakshmi, M., 1999. Genetic diversity in mangrove species of the family Rhizophoraceae. Ph.D. Thesis, University of Madras, Madras.
Nadimpalli, B.G., R.L. Jarret, S.C. Pathak & G. Kochert, 1992. Phylogenetic relationship of pigeonpea (Cajanus cajan) based on nuclear restriction fragment length polymorphisms. Genome 36: 216-223.
Palmer, J.D. & D. Zamir, 1982. Chloroplast DNA evolution and phylogenetic relationships in Lycopersicon. Proc Natl Acad Sci USA 79: 5006-5010.
Palmer, J.D., 1987. Chloroplast DNA evolution and biosystematic uses of chloroplast DNA variation. Am Nat 130: S6-S29.
Palmer, J.D., R.K. Jansen, H.J. Michaels, M.W. Chase & J.R. Manhart, 1988. Chloroplast DNA variation and plant phylogeny. Annal Missou Bot Gar 75: 1180-1206.
Parani, M., M. Lakshmi, B. Ziegenhagen, M. Fladung, P. Senthilkumar & A. Parida, 2000. Molecular phylogeny of Mangroves VII. PCR-RFLP of trnS-psbC and rbcL gene regions in 24 mangrove and mangrove associated species. Theor Appl genet 100: 454-460.
Polhill, R.M., P.H. Raven & C.H. Stirton, 1981. Evolution and systematics of the Leguminosae. In: R.M. Polhill and P.H. Raven (Eds.), Advances in Legume Systematics, pp. 1-26.
Pundir, R.P.S. & R.B. Singh, 1985a. Biosystematic relationship among Cajanus, Atylosia, and Rhynchosia species and evolution of pigeonpea (Cajanus cajan (L.)Millsp.). Theor Appl Genet 69: 531-534.
Pundir, R.P.S. & R.B. Singh, 1985b. Cytogenetics of F1 hybrids between Cajanus, and Atylosia species and its phylogenetic implications. Theor Appl Genet 71: 216-220.
Ratnaparkhe, M.B., V.S. Gupta & P.K. Ranjekar, 1995. Genetic fingerprinting of pigeonpea [Cajanus cajan (L.) Millsp.] and its wild relatives using RAPD markers. Theor Appl Genet 91: 893-898.
Saghai-Maroof, M.A., K.M. Soliman, R.A. Jorgensen & R.A. Allard, 1984. Ribosomal DNA spacer length polymorphism in Barley. Mendelian inheritance, chromosomal locations and population dynamics. Proc Natl Acad Sci USA 81: 8014-8018.
Sambrook, J., E.F. Fritsch & T. Maniatis, 1989. Molecular Cloning, A Laboratory Manual. Cold Spring Harbor Lab Press, USA.
Tsumura, Y., K. Yoshimura, N. Tomaru & K. Ohba, 1995. Molecular phylogeny of conifers using RFLP analysis of PCR-amplified specific chloroplast genes. Theor Appl Genet 91: 1222-1236.
van der Maesen, L.J.G., 1980. India is the native home of Pigeonpea, In: J.C. Arends, G. Boelema, C.T. de Groot & A.J.M. Leeuwenberg (Eds.), Libergratulatorius in honorem H.C.D. de Wit, pp. 257-262. Landebouwhogeschool Miscellaneous Paper no. 19, Wageningen, Netherlands: H. Veenman and B.V. Zonen.
van der Maesen, L.J.G., 1983. World distribution of Pigeonpea. ICRISAT information bulletin no. 14. Patencheru, A.P., India: ICRISAT, pp. 40.
van der Maesen, L.J.G., 1986. Cajanus DC. and Atylosia W.& A. (Leguminosae). Agricultural University Wageningen papers, (1985). Agricultural University, Wageningen, the Netherlands. pp. 225.
van der Maesen, L.J.G., 1990. Pigeonpea: Origin, history, evolution, and taxonomy. In: Y.L. Nene, Susan D. Hall and V.K. Sheila (Eds.), The Pigeonpea, CAB International, University Press, Cambridge, London.
Ziegenhagen, B. & M. Fladung, 1997. Variation in the psbC gene region of gymnosperms and angiosperms as detected by a single restriction site polymorphism. Theor Appl Genet 94(8): 1065-1071.
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Lakshmi, M., Senthilkumar, P., Parani, M. et al. PCR-RFLP analysis of chloroplast gene regions in Cajanus (Leguminosae) and allied genera. Euphytica 116, 243–250 (2000). https://doi.org/10.1023/A:1004030207084
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DOI: https://doi.org/10.1023/A:1004030207084