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Flow cytometric analysis of DNA content in Hosta reveals ploidy chimeras

Abstract

Nuclear DNA content in 84 Hosta cultivars was measuredby flow cytometry. As an internal standard we used the closely related Agave americana, the latter was calibrated against other reference species. Propidium iodide values were 20%higher than DAPI values, indicating a lower AT/GC ratio for Hosta. Ten new tetraploid plants were found in addition to the only tetraploid known so far; H. ventricosa.Small forms of the tetraploid H. ventricosa turned out tobe triploid hybrids. Three other triploids were discovered. Threesupposed tetraploids turned out to be diploid. In five plantsthe ploidy levels of the germ layers L1 and L3 appeared to be different. In these cases thick leaves coincides with a tetraploid DNA content. The DNA contents as measured in theseploidy chimeras coincide with differences in leaf colour, thusgiving an opportunity to analyze the build-up of Hosta plants.In general, analysis of the nuclear DNA content can be used as a simple means to increase the possibilities for creating new cultivars in this economically important perennial.

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References

  • Bennett, M.D. & I.J. Leitch, 1995. Nuclear DNA amounts in Angiosperms. Ann of Bot 76: 113–176.

    Google Scholar 

  • Bennett, M.D. & I.J. Leitch, 1997. Nuclear DNA amounts in Angiosperms-583 new estimates. Ann of Bot 80: 169–196.

    Google Scholar 

  • Bharathan, G., G. Lambert & D.W. Galbraith, 1994. Nuclear DNA content of monocotyledons and related taxa. Am J Bot 81: 381–386.

    Google Scholar 

  • Bogler, D.J. & B.B. Simpson, 1996. Phylogeny of Agavaceae based on its rDNA sequence variation. Am J Bot 83: 1225–1235.

    Google Scholar 

  • Buitendijk, J.H., E.J. Boon & M.S. Ramanna, 1997. Nuclear DNA content in twelve species of Alstroemeria L. and some of their hybrids. Ann Bot 79: 343–353.

    Google Scholar 

  • D'Amato, F., 1964. Endopolyploidy as a factor in plant tissue development. Caryologia 17: 41–51.

    Google Scholar 

  • Cavallini, A., L. Natali, G. Cionini & I. Castorena-Sanchez, 1996. Cytophotometric and biochemical analyses of DNA in pentaploid and diploid Agave species. Genome 39: 266–271.

    Google Scholar 

  • Doležel, J., S. Sgorbati & S. Lucretti, 1992. Comparison of three DNA fluorochromes for flow cytometric estimation of nuclear DNA content in plants. Physiol Plant 85: 625–631.

    Google Scholar 

  • Doležel, J., J.G. Greilhuber, S. Lucretti, A. Meister, M.A. Lysak & R. Obermayer, 1998. Plant genome size estimation by flow cytometry: inter-laboratory comparison. Ann Bot 82 (Suppl A): 17–26.

    Google Scholar 

  • Hanstein, J., 1868. Die Scheitelzellgruppe im Vegetationspunkt der Phanerogamen. Festschr Niederrhein Ges Natur und Heilk: 109–134.

  • Hirao, S., 1981. Inducing tetraploids. The American Hosta Society Bulletin 12: 36.

    Google Scholar 

  • Kaneko, K., 1968. Cytological studies on some species of Hosta III. Karyotypes of H. clausa, H. clausa v. normalis and H. ventricosa. Bot Mag Tokyo 81: 396–403.

    Google Scholar 

  • De Laat, A.N.M., W. Göhde & M.J.D. Vogelzang, 1987. Determination of ploidy of single plants and plant populations by flow cytometry. Plant Breeding 99: 303–307.

    Google Scholar 

  • Neilson-Jones, W., 1969. Plant Chimeras 1–124. Methuen & Co. London.

    Google Scholar 

  • Poehtig, S., 1989. Genetic mosaics and cell lineage analysis in plants. Trend Genet 5: 273–277.

    Google Scholar 

  • Satina, S., A.F. Blakeslee & A.G. Avery, 1940. Demonstration of the three germ layers in the shoot apex of Datura by means of induced polyploidy in periclinal chimeras. Am J Bot 27: 895–905.

    Google Scholar 

  • Schmid, G.H., 1991. The genus Hosta. Timber Press, Portland, Oregon.

    Google Scholar 

  • Stewart, R.N. & H. Dermen, 1979. Ontogeny in monocotyledons as revealed by studies of the developmental anatomy of periclinal chloroplast chimeras. Am J Bot 66: 47–58.

    Google Scholar 

  • The genus Hosta. List of registered Cultivars, 1993 and yearly additions. ed The American Hosta Society.

  • Tilney-Bassett, R.A.E., 1963. The structure of periclinal chimeras. Heredity 18: 265–294.

    Google Scholar 

  • Torrey, J.G., 1961. Kinetin as trigger for mitosis in mature endomitotic plant cells. Exp Cell Res 23: 281–299.

    PubMed  Google Scholar 

  • Winkler, H., 1907. Über Pröpfbastarde und Pflanzliche Chimären. Ber Deutsch Bot Ges 25: 568–576.

    Google Scholar 

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Zonneveld, B.J., Van Iren, F. Flow cytometric analysis of DNA content in Hosta reveals ploidy chimeras. Euphytica 111, 105–110 (2000). https://doi.org/10.1023/A:1003879408413

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  • DOI: https://doi.org/10.1023/A:1003879408413

  • flow cytometry
  • Hosta
  • ploidy chimeras
  • propidium iodide