Abstract
The presence of endocrine cells in the gills of the goldfish Carassius carassius and the effects of lead intoxication (5 mg l−1) on their relative abundance and secretory activity were studied. Endocrine cells synthesizing peptido-like (met and leu-enkephalin and neuropeptide Y) and serotonin-like substances were detected in gill filaments by immunocytochemical procedures. Decreased immunoreactivity for two enkephalins and neuropeptide Y was observed after 48 and 96 h lead exposure. In contrast, increased serotonin immunoreactivity was evident after a one-week treatment. A search for Ca++-binding proteins was also carried out by immunocytochemical methods. Calbindin D-28K and S-100-like molecules were detected in gill epithelium, and their expression appeared enhanced after lead exposure.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References cited
Bailly Y, Dunel-Erb S, Laurent P (1992) The neuroepithelial cells of the fish gill filaments: indolamine-immunocytochemistry and innervation. Anat Rec 233: 143-161.
Bolognani Fantin AM, Trevisan P, Pederzoli A, Bergomi M (1988) Effects of acute experimental pollution by lead on some haematological parameters in Carassius carassius (L.) var. auratus. Boll Zool 55: 251-255.
Burkhardt-Holm P (1997) Lectin histochemistry of rainbow trout (Oncorhynchus mykiss) gill and skin. Histochem J 29: 893-899.
Dunel-Erb S (1994) The neuroepithelial cells of the fish gill filament. In Singh BR ed. Advances in Fish Research. Delhi: Narendra.
Dunel-Erb S, Bailly Y, Laurent P (1982) Neuroepithelial cells in fish gill primary lamellae. J Appl Physiol 53: 1342-1353.
Dunel-Erb S, Bailly Y, Laurent P (1989) Neurons controlling the gill vasculature in five species of teleosts. Cell Tiss Res 255: 567-573.
Ferguson HW, Morrison D, Ostland VE, Lumsden J, Byrne P (1992) Responses of mucus-producing cells in gill disease of rainbow trout (Oncorhynchus mykiss). J Comp Pathol 106: 255-265.
Franchini A, Alessandrini F, Bolognani Fantin AM (1994) Gill morphology and ATPase activity in the goldfish Carassius carassius var. auratus exposed to experimental lead intoxication. Boll Zool 61: 29-37.
Goniakowska-Witalinska L, Fasulo S, Mauceri A, Licata A, Youson J (1995) The neuroendocrine cells in the gills of the bowfin Amia calva. An ultrastructural and immunocytochemical study. Folia Histochem Cytobiol 33: 69-75.
Hsu SM, Raine L, Fanger H (1981) Use of avidin-biotin peroxidase complex (ABC) in immunoperoxidase techniques: a comparison between ABC and unlabelled antibody (PAP) procedures. J Histochem Cytochem 29: 577-580.
Kiliaan AJ, Holmgren S, Jonsson AC, Dekker K, Groot JA (1993) Neuropeptides in the intestine of two teleosts species (Oreochromis mossambicus, Carassius auratus): localization and electrophysiological effects on the epithelium. Cell Tiss Res 271: 123-134.
Laurent P (1984) Gill internal morphology. In Hoar WS, Randall DJ ed. Fish Physiology, Vol. 10a. New York: Academic Press, pp. 73-183.
Laurent P (1989) Gill structure and function: Fish. In Wood SC ed. Comparative Pulmonary Physiology, Current Concepts New York: Dekker, pp. 69-120.
Laurent P, Dunel S (1980) Morphology of gill epithelia in fish. Am J Physiol 238: 147-159.
Mallat J (1985) Fish gill structural changes induced by toxicants and other irritants: a statistical review. Can J Fish Aquat Sci 42: 630-648.
Pisam M, Rambourg A (1991) Mitochondria-rich cells in the gill epithelia of teleost fishes: an ultrastructural approach. Int Rev Cytol 130: 191-232.
Pisam M, Boeuf G, Prunet P, Rambourg A (1990) Ultrastructural features of mitochondria-rich cells in stenohaline freshwater and seawater fishes. Am J Anat 187: 21-31.
Playle RC, Wood CM (1989) Water chemistry changes in the gill environment of rainbow trout-experimental observations and theory. J Comp Physiol B 159: 527-537.
Wendelaar-Bonga SE (1993) Endocrinology. In Evans DH ed. The Physiology of Fishes. Boca Raton, FL: CRC Press, pp. 469-502.
Young JZ (1932-1933) The preparation of isotonic solutions for use in experiments with fish. Pubbl Staz Zool Napoli 12: 425-431.
Zaccone G, Fasulo S, Ainis L (1994) Distribution patterns of the paraneuronal endocrine cells in the skin, gills and the airways of fishes as determined by immunohistochemical and histological methods. Histochem J 26: 609-629.
Zaccone G, Fasulo S, Ainis L (1995) Neuroendocrine epithelial cell system in respiratory organs of air-breathing and teleost fishes. Int Rev Cytol 157: 277-314.
Zaccone G, Lauweryns JM, Fasulo S, Tagliafierro G, Ainis L, Licata A (1992a) Immunocytochemical localization of serotonin and neuropeptides in the neuroendocrine paraneurons of teleost and lungfish gills. Acta Zool (Stockholm) 73: 177-183.
Zaccone G, Wendelaar-Bonga SE, Flik G, Fasulo S, Licata A, Lo Cascio P, Mauceri A, Lauriano ER (1992b) Localization of calbindin D28Klike immuno-reactivity in fish gill: a light microscopic and immuno-electron histochemical study. Regul Pept 41: 195-208.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Franchini, A., Rebecchi, B. & Fantin, A.M.B. Gill Endocrine Cells in the Goldfish Carassius carassius var. auratus and Their Impairment Following Experimental Lead Intoxication. Histochem J 31, 559–564 (1999). https://doi.org/10.1023/A:1003848225503
Issue Date:
DOI: https://doi.org/10.1023/A:1003848225503