Abstract
Genetic diversity within and among populations was investigated using RAPD-PCR assays in a rare species, Afgekia sericea Craib. Two hundred and sixty-nine individuals were sampled from nine geographically isolated populations from northeastern Thailand. This study includes 73 RAPD markers. Within population polymorphism as measured by percentage of polymorphic RAPDs, varied between 38.4% and 60.3%. Genetic variability was measured using Shannon's information index and partitioned into between- and within-population components. Overall, genetic variation among A. sericea populations was high (Hsp-Hpop)/Hsp=48.2%. The genetic diversity for the species (Hsp) was 43.2%. Mean of within-population values (Hpop) for all populations was 0.224. The total genetic diversity was explained by high variation among populations (mean Gst=0.426), which is consistent with low gene flow among populations (Nm=0.35). High between-population genetic variation observed in this study could be explained by limited migration through seed and/or pollen dispersal among populations. Conservation strategies of A. sericea are discussed in the context of these results.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Ashburner, G. R., W.K. Thompson & G.M. Halloran, 1997. RAPD analysis of South Pacific coconut palm populations. Crop Sci. 37: 992–997.
Beardmore, J.A., 1983. Extinction, survival, and genetic variation, pp. 125–151 in Genetics and Conservation, edited by C.M. Schonewald-Cox, S.M. Chambers, B. MacBryde and W.L. Thomas. Benjamin / Cummings, Menlo Park, CA.
Bonnin, I., T. Huguet, M. Gherard, M. Prosperi & I. Olivieri, 1996. High level of polymorphism and spatial structure in a selfing plant species, Medicago truncatula (Leguminosae), showing using RAPD markers. Am. J. Bot. 83: 843–855.
Burtt, B. L. & C. Chermsirivathana, 1971. A second species of Afgekia (Leguminosae). Note from the Royal Botanic Garden Edinburgh 31: 131–133.
Chakraborty, R. & C.R. Rao, 1991. Measurements of genetic variation for evolutionary studies, pp. 271–316 in Handbook of Statistics, Vol. 8, edited by C. R. Rao and R. Chakraborty. Elsevier, Amsterdam, The Netherlands.
Chalmers, K., J.R. Waugh, J.I. Sprent, A.J. Simons & W. Powell, 1992. Detection of genetic variation between-and within-populations of Gliricidia sepium and G. maculata using RAPD markers. Heredity 69: 465–472.
Chuakul, W., S. Prathanturarug, P. Saralamp & P. Supatarawanich, 1994. Medicinal plants in Mahasarakham Province. Thai Phytopharm. 1: 39–56.
Crow, J. F. & K. Aoki, 1984. Group selection for a polygenic behavioral trait: Estimating the degree of population subdivision. Proc. Natl. Acad. Sci. USA 81: 6073–6077.
Doyle, J. J. & J.L. Doyle, 1987. A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochem. Bull. 19: 11–15.
Ellstrand, N. C. & D.R. Elam, 1993. Population genetic consequences of small population size: Implications for conservation. Ann. Rev. Ecol. Syst. 24: 217–242.
Felsenstein, J., 1993. PHYLIP (Phylogeny Inference Package), version 3.5c edition. University of Washington, Seattle, Washington.
Foster, P.F. & V.L. Sork, 1997. Population and genetic structure of the West African rain forest liana Ancistrocladus korupensis (Ancistrocladaceae). Am. J. Bot. 84: 1078–1091.
Godt, M.J.W. & J.L. Hamrick, 1996. Genetic structure of two endangered pitcher plants, Sarracenia jonesii and Sarracenia oreophila (Sarraceniaceae). Am. J. Bot. 83: 1016–1023.
Gustafsson, L. & P. Gustafsson, 1994. Low genetic variation in Swedish populations of the rare species Vicia pisiformis (Fabaceae) revealed with rflp (r DNA) and RAPD. Plant Syst. Evol. 189: 133–148.
Henry, R. J., 1997. Practical Applications of Plant Molecular Biology. Chapman & Hall, London.
Heywood, J., 1991. Spatial analysis of genetic variation in plant populations. Ann. Rev. Ecol. Syst. 22: 335–355.
Hodges, S.A. & M.L. Arnold, 1994. Floral and ecological isolation between Aquilegia formosa and Aquilegia pubescens. Proc. Natl. Acad. Sci. USA 91: 2493–2496.
Holsinger, K E. & L.D. Gottlieb, 1991. Conservation of rare and endangered plants: principles and prospect, pp. 195–208 in Genetics and Conservation of Rare Plants, edited by D. A. Falk and K.E. Holsinger. Oxford University Press, NY.
Hsiao, J. Y. & L.H. Rieseberg, 1993. Genetic variability of a population of Yushania niitakayamensis (Bambusoideae, Poaceae) in Taiwan as revealed by RAPDs. Am. J. Bot. 80(Suppl.): 56.
Huen, M., J.P. Murphy & T.D. Phillips, 1994. A comparison of RAPD and isozyme analyses for determining the genetic relationships among Avena sterilis L. accessions. Theor. Appl. Genet. 87: 689–696.
Huff, D. R., R. Peakall & P.E. Smouse, 1993. RAPD variation within and among natural populations of outcrossing buffalo-grass [Buchloe dactyloides (Nutt.) Engelm]. Theor. Appl. Genet. 86: 927–934.
Lanham, P.G., 1996. Estimation of heterozygosity in Ribes nigrum L. using RAPD markers. Genetica 98: 193–197.
Lin, T. P., Y.P. Cheng & S.G. Huang, 1997. Allozyme variation in four geographic areas of Cinnamomum kanehirae. J. Hered. 88: 433–438.
Link, W., C. Diskens, M. Singh, M. Schwall & A.E. Melchinger, 1995. Genetic diversity in European and Mediterranean faba bean germplasm revealed by RAPD markers. Theor. Appl. Genet. 90: 27–32.
McDonald, B. A. 1997. The population genetics of fungi: Tools and techniques. Phytopathology 87: 448–453.
Nei, M., 1973. Analysis of gene diversity in subdivided populations. Proc. Natl. Acad. Sci. USA 70: 3321–3323.
Nei, M., 1978. Estimation of average heterozygosity and genetic distance from a small number of individuals. Genetics 89: 583–590.
Nei, M., 1987. Molecular Evolutionary Genetics. Columbia University Press, New York, NY.
Peever, T. L. & M.G. Milgroom, 1994. Genetic structure of Pyrenophora teres populations determined with random amplified polymorphic DNA markers. Can. J. Bot. 72: 915–923.
Prathepha, P., 1990. Ecological Genetics of the Genus Afgekia Craib in Thailand. M.Sc. Thesis, Chulalongkorn University, Bangkok, Thailand.
Prathepha, P., 1994. Chromosome number of the genus Afgekia Craib (Leguminosae). Cytologia 59: 437–438.
Rossetto, M., P.K. Weaver & K.W. Dixon, 1995. Use of RAPD analysis in devising conservation strategies for the rare and endangered Grevillea scapigera (Proteaceae). Mol. Ecol. 321–329.
Russell, J. R., F. Hosein, E. Johnson, R. Waugh & W. Powell, 1993. Genetic differentiation of cocoa (Theobroma cacao L.) populations revealed by RAPD analysis. Mol. Ecol. 2: 89–97.
Waugh, R. & W. Powell, 1992. Using RAPD markers for crop improvement. TIBTECH 10: 186–191.
Williams, J.G.K., A.R. Kubelik, K.J. Livak, J.A. Rafalski & S.V. Tingey, 1990. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Research 18: 6531–6535.
Yeh, F. C. & T. Boyle, 1997. POPGENE, Microsoft Window-based Software for Population Genetic Analysis. Release 1.2. A joint project development by Francis C. Yeh, University Alberta and Tim Boyle, Center for International Forestry Research, Bogor, Indonesia.
Yu, K. & K.P. Pauls, 1994. Optimization of DNA-extraction and PCR procedures for random amplified polymorphic DNA (RAPD) analysis in plants, pp. 193–200 in PCR Technology: Current Innovations, edited by H. G. Griffin and A. M. Griffin. CRC Press, Boca Raton, FL.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Prathepha, P., Baimai, V. Genetic differentiation in Thai populations of the rare species Afgekia sericea Craib (Leguminosae) revealed by RAPDPCR assays. Genetica 105, 193–202 (1999). https://doi.org/10.1023/A:1003762802997
Issue Date:
DOI: https://doi.org/10.1023/A:1003762802997