Skip to main content
SpringerLink
Log in

Suppression of 125I-vasoactive Intestinal Polypeptide Binding Sites in Arteries of the Hamster Seminal Vesicle Following Castration

  • Published:
The Histochemical Journal Aims and scope Submit manuscript

Abstract

The presence and distribution of 125I-vasoactive intestinal polypeptide (VIP) binding sites in blood vessels supplying the hamster seminal vesicle was studied using a receptor autoradiographic technique before and following castration. 125I-VIP binding was studied in intact animals, in animals under a 15-day period of castration and in animals under the same period of castration but submitted to a further 15-day period of testosterone treatment.

Our results show that, in the seminal vesicle, VIP-binding sites are localized in the gland smooth muscle coat and arterial smooth muscle. A 15-day castration period abolishes 125I-VIP binding to vascular smooth muscle but has no effect on 125I-VIP binding to the gland smooth muscle coat. Treatment with testosterone restores 125I-VIP binding to the vascular smooth muscle, completely reversing the effect of castration.

Our results indicate that VIP-binding sites in the smooth muscle wall of arteries supplying the hamster seminal vesicle are under androgenic control and are more sensitive to androgen deprivation that VIP-binding sites associated to the gland smooth muscle coat.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References cited

  • Adamou JE, Aiyar N, Van Horn S. Elshourbagy NA (1996) Cloning and functional characterization of the human vasoactive intestinal peptide (VIP)-2 receptor. Biochem Biophys Res Commun 209: 385-392.

    Google Scholar 

  • Carmena MJ, Recio, MN, Prieto JC (1988) Influence of castration and testosterone treatment on the vasoactive intestinal peptide receptor/effector system in rat prostatic epithelial cells. Biochim Biophys Acta 969: 86-90.

    Google Scholar 

  • Couvineau A, Rouyer-Fessard C, Darmoul D, Maoret J, Carrero I, Ogier-Denis E, Laburthe M (1994) Human intestinal VIP receptor: cloning and functional expression of two cDNAs encoding proteins with different N-terminal domains. Biochem Biophys ResCommun 200: 769-776.

    Google Scholar 

  • Dail WG (1993) Autonomic innervation of male reproductive genitalia. In Nervous Control of the Urogenital System (ed. C.A. Maggi) Chur, Switzerland: Harwood academic publishers, pp. 69-101.

    Google Scholar 

  • Dieudonné M-N, Pecquery R, Leneveu M-C, Dausse J-P, Giudicelli Y (1994) In vitro up-regulatory effect of testosterone on α2-adrenoceptor expression in adipose tissue. Endocrine 2: 567-570.

    Google Scholar 

  • Fahrenkrug J, Palle C, Jørgensen J, Ottesen B (1989) Regulatory peptides in the mammalian urogenital system. In Regulatory Peptides (ed. J.M. Polak), Basal: Birkhäuser Verlag, pp. 362-381.

    Google Scholar 

  • Guijarro LG, Rodriguez-Pena MS, Prieto JC (1991) Characterization of vasoactive intestinal peptide receptors in rat seminal vesicle. Am J Physiol 260: E286-E291.

    Google Scholar 

  • Hill JM, Harris A, Hilton-Clarke DI (1992) Regional distribution of guanine nucleotide-sensitive and guanine nucleotide-insensitive vasoactive intestinal peptide receptors in rat brain. Neuroscience 48: 925-932.

    Google Scholar 

  • Huang M, Rorstad OP (1987) VIP receptors in mesenteric and coronary arteries: a radioligand binding study. Peptides 8: 477-485.

    Google Scholar 

  • Lundberg JM, Anggard A, Fahrenkrug J, Hokfelt T, Mutt V (1980) Vasoactive intestinal polypeptide in cholinergic neurons on exocrine glands: functional significance of coexisting transmitters for vasodilation and secretion. Proc Natl Acad Sci USA 77: 1651-1655.

    Google Scholar 

  • Luke MC, Coffey DS (1994) The male sex accessory tissues. Structure, androgen action, and physiology. In The Physiology of Reproduction (E Knobil and JD Neil, eds.), 2nd Edn., London: Academic Press, pp. 469-500.

    Google Scholar 

  • Luu TN, Dashwood MR, Chester AH, Tadjkarimi S, Yacoub MH (1993) Action of vasoactive intestinal peptide and distribution of its binding sites in vessels used for coronary artery bypass grafts. Am J Cardiol 71: 1278-1282.

    Google Scholar 

  • Mata L (1995) Dynamics of the seminal vesicle epithelium. Int Rev Cytol 160: 267-302.

    Google Scholar 

  • Opgaard OS, Edvinsson L (1996) Effect of parasympathetic and sensory transmitters on human epicardial coronary arteries. Pharmacol Toxicol 78: 273-279.

    Google Scholar 

  • Pinho MS, Mata LR (1992) Castration-resistant secretion in the hamster seminal vesicle does not depend on androgens. Int J Androl 15: 435-437.

    Google Scholar 

  • Pinho MS, Sebastião AM, Rodrigues G, Barroso CP, Ribeiro JA, Mata LR, Gulbenkian S (1994) Vasoactive intestinal peptide in the hamster seminal vesicle: Distribution, binding sites and possible functions. Neuroscience 59: 1083-1091.

    Google Scholar 

  • Pinho MS, Afonso F, Fernandes P, Rodrigues G, Mata LR, Gulbenkian S (1996) Effect of castration on the VIPergic innervation and 125I-labelled vasoactive intestinal peptide (VIP) binding sites in the hamster seminal vesicle. A quantitative immunohistochemical and receptor autoradiographic study. Regul Pept 66: 169-177.

    Google Scholar 

  • Robberecht P, Coy DH, De Neef P, Camus J-C, Cauvin A, Waelbroeck M, Christophe J (1986) Interaction of vasoactive intestinal peptide (VIP) and N-terminally modified VIP analogs with rat pancreatic, hepatic and pituitary membranes. Eur J Biochem 159: 45-49.

    Google Scholar 

  • Robberecht P, De Neef P, Gourlet P, Cauvin A, Coy DH, Christophe J (1988a) Pharmacological characterization of the novel helodermin/ VIP receptor present in human SUP-T1 lymphoma cell membranes. Regul Pept 26: 117-126.

    Google Scholar 

  • Robberecht P, Waelbroeck M, De Neef P, Tastenoy M, Gourlet P, Cogniaux J, Christophe J (1988b) A new type of functional VIP receptor has an affinity for halodermin in human SUP-T1 lymphoblasts. FEBS Lett 228: 351-355.

    Google Scholar 

  • Rodriguez-Pena MS, Guijarro LG, Prieto JC (1991) Adenylyl cyclase stimulation by VIP in rat seminal vesicle membranes. Peptides 12: 821-824.

    Google Scholar 

  • Rorstad OP, Wanke I, Coy DH, Fournier A, Huang M (1990) Selectivity for binding of peptide analogs to vascular receptors for vasoactive intestinal peptide. Mol Pharmacol 37: 971-977.

    Google Scholar 

  • Rostene W, Dussaillant M, Denis P, Gozes I, Montagne MN, Berod A (1992) Distribution and regulation of vasoactive intestinal peptide mRNA and binding sites in the mammalian eye, brain and pituitary. Biomed Res 13: 63-69.

    Google Scholar 

  • Staun-Olsen P, Ottesen B, Gammeltoft S, Fahrenkrug J (1986) VIP binding sites on synaptosomes from rat cerebral cortex: structure-binding relationship. Peptides 7: 181-186.

    Google Scholar 

  • Stjernquist M, Häkanson R, Leander S, Owman C, Sundler F, Uddman R (1983) Immunohistochemical localization of substance P, vasoactive intestinal polypeptide and gastrin releasing peptide in the vas deferens and seminal vesicle and the effect of these and eight other neuropeptides on resting tension and neurally evoked contractile activity. Regul Pept 7: 67-86.

    Google Scholar 

  • Usdin TB, Bonner TI, Mezey E (1994) Two receptors for vasoactive intestinal polypeptide with similar specificity and complementary distributions, Endocrinology 135: 2662-2680.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Gulbenkian Institute of Science, Apartado 14, 2781, Oeiras, Portugal

    C.P. Barroso, F. Afonso, M. Pinho & S. Gulbenkian

  2. Department of Morphology and Clinics, Faculty of Veterinary Medicine, Lisbon, Portugal

    F. Afonso & M. Pinho

  3. Department of Zoology and Anthropology, Faculty of Sciences, University of Lisbon, Lisbon, Portugal

    L. Mata

Authors
  1. C.P. Barroso
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. F. Afonso
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. M. Castro Caldas
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. M. Pinho
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. L. Mata
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. S. Gulbenkian
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Barroso, C., Afonso, F., Castro Caldas, M. et al. Suppression of 125I-vasoactive Intestinal Polypeptide Binding Sites in Arteries of the Hamster Seminal Vesicle Following Castration. Histochem J 31, 271–276 (1999). https://doi.org/10.1023/A:1003747716200

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1003747716200

Keywords

Search

Navigation