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The effect of ovule age on ovary-slice culture and ovule culture in intraspecific and interspecific crosses with Tulipa gesneriana L.

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Abstract

The efficiency of two embryo rescue techniques, direct ovule culture and ovary-slice culture followed by ovule culture, is studied in crosses with Tulipa gesneriana as maternal genotype. The germination percentages increased, in most cases, significantly with increasing ages of te ovary-slices and ovules at the start of the cultures. The low number of embryos recovered at early culture dates is caused by a higher rate of embryo abortion and by retarded embryo development. The germination percentages for ovary-slice culture followed by ovule culture was mostly comparable to direct ovule culture. Unique hybrids have been obtained from the crosses T. gesneriana × T. agenensis and T. gesneriana × T. praestans.

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Authors and Affiliations

  1. Centre for Plant Breeding and Reproduction Research (CPRO-DLO), P.O. Box 16, 6700 AA, Wageningen, The Netherlands

    M.G.M. Van Creij, D.M.F.J. Kerckhoffs & J.M. Van Tuyl

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  1. M.G.M. Van Creij
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  2. D.M.F.J. Kerckhoffs
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  3. J.M. Van Tuyl
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Van Creij, M., Kerckhoffs, D. & Van Tuyl, J. The effect of ovule age on ovary-slice culture and ovule culture in intraspecific and interspecific crosses with Tulipa gesneriana L.. Euphytica 108, 21–28 (1999). https://doi.org/10.1023/A:1003644009572

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