Abstract
A genic male-sterility gene newly induced by chemical mutagenesis, tentatively designated as ms-h(t), was located on the molecular map of rice and tested for its effect on chalky endosperm. Bulked segregant analysis was used to determine the chromosomal location of the ms-h(t) locus by screening four to five RFLP markers per chromosome. After confirming that the gene was located on chromosome 9, twenty-four RFLP markers from chromosome 9 were surveyed for polymorphism in the parents of the mapping population. Of these, eleven markers were mapped around the ms- h(t) locus. RG451 and RZ404 flanked the ms-h(t) gene, at 2.5cM and 3.3cM, respectively. Heterozygous F2 to F4 progenies were tested for co- segregation of male-sterility and chalky endosperm and it was revealed that ms-h(t) might have a pleiotropic effect on chalky endosperm. This mutant would be a good biological material to characterize the biochemical mechanism of male sterility and related pleiotropic effects. Further studies should be needed to know the usefulness of this mutant for hybrid seed production.
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Causse, M.A., T.M. Fulton, Y.G. Cho, S.N. Ahn, J. Chunwongse, K. Wu, J. Xiao, Z. Yu, P.C. Ronald, S.E. Harrington, G. Second, S.R. McCouch & S.D. Tanksley, 1994. Saturated molecular map of the rice genome based on an interspecific backcross population. Genetics 138: 1251–1274.
Feinberg, A.P. & B. Vogelstein, 1983. A technique for radiolabelling DNA restriction endonuclease fragments to high specific activity. Anal Biochem 132: 6–13.
Galinat, W.C., 1975. Use of male sterile 1 gene to eliminate detasseling in production of hybrid seed of bicolor sweet corn. J Hered 66: 387–388.
Horner, H.T. & R.G. Palmer, 1995. Mechanisms of genic male sterility. Crop Sci 35: 1527–1535.
Hoshikawa, K., 1993. Rice grain texture and grading standard. In: T. Matsuo & K. Hoshilawa (Eds), 'Science of the Rice Plant, pp. 383–389. Vol.1. Morphology, Food and Agriculture Policy Research Center, Tokyo, Japan.
Kaul, M.L.H., 1988. Male Sterility in Higher Plant. Springer-Verlag, pp. 15–96.
Kinoshita, T., 1995. Report of committee on gene symbolization, nomenclature and linkage groups. Rice Genet Newsl 12: 9–153.
Ko, T. & H. Yamagata, 1987. Gene analysis of male sterility induced in rice. Studies on the utility of artificial mutations in plant breeding. XVI. Jpn J Breed 37: 192–198.
Ko, T. & H. Yamagata, 1989. Studies on the induction of malesterile strains in rice. 9. Linkage groups of a few male-sterility genes. Jpn J Breed 39 (Suppl. 1): 234–235.
Koh, H.J. & M.H. Heu, 1996. Agronomic characteristics of a mutant for genic male sterility-chalky endosperm and its utilization on F1 hybrid breeding system in rice. Korean J Crop Sci 40(6): 684–696.
Koh, H.J., M.H. Heu & S.R. McCouch, 1996. Molecular mapping of the ges gene controlling the super-giant embryo character in rice (Oryza sativa L.). Theor Appl Genet 92: 257–261.
Kosambi, D.D., 1944. The estimation of map distances from recombination values. Ann Eugenet 12: 172–175.
Lander, E.S., P. Green, J. Abrahamson, A. Barlow, M.J. Daly, S.E. Lincoln & L. Newburg, 1987. MAPMAKER: An interactive computer package for constructing primary genetic linkage maps of experimental and natural populations. Genomics 1: 174–181.
Lu, X.G., Z.G.Zhang, K. Maruyama & S.S. Virmani, 1994. Current status of two-line method of hybrid rice breeding. In: S.S. Virmani (Ed), Hybrid Rice Technology — New Developments and Future Prospects, pp. 37–49. IRRI.
McCouch, S.R., G. Kochert, Z.H. Yu, Z.Y. Wang, G.S. Khush, W.R. Coffman & S.D. Tanksley, 1988. Molecular mapping of rice chromosomes. Theor Appl Genet 76: 815–829.
Michelmore, R.W., I. Paran & K.V. Kesseli, 1991. Identification of markers linked to disease resistance genes by bulked segregant analysis: a rapid method to detect markers in specific genomic regions by using segregating populations. Proc Natl Acad Sci USA 88: 9828–9832.
Suh, H.S., J.D. Kim, M.H. Heu & G.S. Chung, 1991. Linkage relationships between genetic male sterile and marker genes in rice. In Rice Genetics II, pp. 121–125. IRRI.
Trees, S.C. & J.N. Rutger, 1978. Inheritance of four genetic male steriles. J Hered 69: 270–272.
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Koh, H., Son, Y., Heu, M. et al. Molecular mapping of a new genic male-sterility gene causing chalky endosperm in rice ( Oryza sativa L.). Euphytica 106, 57–62 (1999). https://doi.org/10.1023/A:1003575016035
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DOI: https://doi.org/10.1023/A:1003575016035