Abstract
The short-chained type X collagen was once thought to be produced exclusively by hypertrophic chondrocytes during endochondral ossification. More recently, however, it has been found elsewhere, for example in articular cartilage. In the present study, the occurrence of type X collagen in the intervertebral disc has been investigated. Human disc tissues of varying pathologies were examined for the presence of type X collagen and expression of alpha1(X) mRNA by immunohistochemistry and in situ hybridization respectively. All samples of disc contained areas that were immunoreactive but to varying extents. In the disc itself, staining for the protein and alpha1(X) mRNA was seen frequently associated with cells of the nucleus pulposus, which were large and of hypertrophic appearance, most commonly found in degenerate discs, and also in areas of disorganized architecture, such as clefts. In addition, type X collagen, both protein and mRNA, was found in regions of the cartilage end-plate, which calcify ectopically in scoliotic patients. We suggest that type X collagen production may be a response of disc tissue cells to a stimulus, such as altered loading. © 1998 Chapman & Hall
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Aigner, T., Reichenberger, E., Bertling, W., Kirsch, T., Stob, H. & von der Mark, K. (1993) Type X collagen expression in osteoarthritic and rheumatoid articular cartilage. Virchows Archiv B Cell Pathol. 63, 205-11.
Alini, M., Carey, D., Hirata, S., Grynpas, M.D., Pi-Doux, I. & Poole, R. (1994) Cellular and matrix changes before and at the time of calcification in the growth plate studied in vitro: arrest of type X collagen synthesis and net loss of collagen when calcification is initiated. J. Bone Miner. Res. 9, 1077-87.
Bonen, D.K. & Schmid, T.M. (1991) Elevated extracellular calcium concentrations induce type X collagen synthesis in chondrocyte cultures. J. Cell Biol. 115, 1171-8.
Carey, D.E. & Liu, X. (1995) Expression of bone morphogenetic protein-6 messenger RNA in bovine growth plate chondrocytes of different size. J. Bone Miner. Res. 10, 401-5.
Chen, Q., Linsenmayer, C., Gu, H., Schmid, T.M. & Linsenmayer, T.F. (1992) Domains of type X collagen: alteration of cartilage matrix by fibril association and proteoglycan accumulation. J. Cell Biol. 117, 687-94.
Claassen, H. & Kirsch, T. (1994) Immunolocalisation of type X collagen before and after mineralization of human thyroid cartilage. Histochemistry 101, 27-32.
Eyre, D.R. (1988) Collagens of the disc. In The Biology of the Intervertebral Disc. Vol. 1 (edited by Ghosh, P. ), pp. 171-88. Boca Raton: CRC Press.
Gardner, D.L. (1992) Osteoarthritis and allied diseases. In Pathological Basis of Connective Tissue Diseases (edited by Gardner, D.L. ), pp. 842-923. London: Edward Arnold.
Gibson, G. J., Bearman, C.H. & Flint, M.H. (1986) The immunoperoxidase localization of type X collagen in chick cartilage and lung. Collagen Res. Rel. 6, 163-84.
Girkontaite, I., Frischholz, S., Lammi, P., Wagner, K., Swoboda, B., Aigner, T. & von der Mark K. (1996) Immunolocalization of type X collagen in normal fetal and adult osteoarthritic cartilage with monoclonal antibodies. Matrix Biol. 15, 231-8.
Hoyland, J.A., Thomas, J.T., Donn, R., Marriott, A., Ayad, S., Boot-Handford, R.P., Grant, M.E. & Freemont, A. J. (1991) Distribution of type X collagen mRNA in normal and osteoarthritic human cartilage. Bone Miner. 15, 151-64.
Kirsch, T., Ishikawa, Y., Mwale, F. & Wuthier, R.E. (1994) Roles of the nucleational core complex and collagens (types II and X) in calcification of growth plate cartilage matrix vesicles. J. Biol. Chem. 269, 20103-9.
Kwan, A.P.L., Dickson, I.R., Freemont, A. J. & Grant, M.E. (1989) Comparative studies of type X collagen expression in normal and rachitic chicken epiphyseal cartilage. J. Cell Biol. 109, 1849-56.
Pritzker, K.P.H. (1977) Aging and degeneration in the lumbar intervertebral disc. Orthop. Clin. N. Am. 8, 65-77.
Reginato, A.M., Tuan, R.S., Ono, T., J imenez, S.A. & Jacenko, O. (1993) Effects of calcium deficiency on chondrocyte hypertrophy and type X collagen expression in chick embryonic sternum. Dev. Dynamics 198, 284-95.
Reichenberger, E., Beier, F., Luvalle, P., Olsen, B.R., von der Mark, K. & Bertling, W.M. (1992) Genomic organization and full length cDNA sequence of human collagen X. FEBS Lett. 311, 305-10.
Roberts, S., Menage, J., Duance, V., Wotton, S. & Ayad, S. (1991) Collagen types around the cells of the intervertebral disc and cartilage end-plate: an immunolocalisation study. Spine 16, 1030-8.
Roberts, S., Menage, J. & Eisenstein, S.M. (1993) The cartilage end-plate and intervertebral disc in scoliosis: calcification and other sequelae. J. Orthop. Res. 11, 747-57.
Rooney, P., Wang, M., Kumar, P. & Kumar, S. (1993) Angiogenic oligosaccharides of hyaluronan enhance the production of collagens by endothelial cells. J. Cell Sci. 105, 213-18.
Rucklidge, G. J., Milne, G. & Robins, S.P. (1996) Collagen Type X: a component of the surface of normal human pig, and rat articular cartilage. Biochem. Biophys. Res. Commun. 224, 297-302.
Schmid, T.M. & Linsenmayer, T.F. (1983) A short chain (pro)collagen from aged epiphysial chondrocytes. Biochemical characterisation. J. Biol. Chem. 258, 9504-9.
Schmid, T.M. & Linsenmayer, T.F. (1985) Developmental acquisition of type X collagen in the embryonic chick tibiotarsus. Dev. Biol. 107, 373-81.
Schmid, T.M., Mayne, R., Jeffrey, J. J. & Linsenmayer, T.F. (1986) Type X collagen contains two cleavage sites for a vertebrate collagenase. J. Biol. Chem. 261, 4184-9.
Schmid, T.M., Popp, R.G. & Linsenmayer, T.F. (1990) Hypertrophic cartilage matrix. Type X collagen, supramolecular assembly, and calcification. Ann. NY. Acad. Sci. 580, 64-73.
Stephens, M., Kwan, A.P.L., Bayliss, M.T. & Archer, C.W. (1992) Human articular surface chondrocytes initiate alkaline phosphatase and type X collagen synthesis in suspension cultures. J. Cell Sci. 103, 1111-16.
Sylvest, J., Hentzer, B. & Kobayasi, T. (1977) Ultrastructure of prolapsed disc. Acta Orthop. Scand. 48, 32-40.
Thomas, J.T., Sweetman, W.A., Cresswell, C. J., Wallis, G.A., Grant, M.E. & Boot-Handford, R.P. (1995) Sequence comparison of three mammalian type X collagen promoters and preliminary functional analysis of the human promoter. Gene 160, 291-6.
Vernon-Roberts, B. (1988) Disc pathology and disease states. In: The Biology of the Intervertebral Disc. Vol. 1 (edited by Ghosh, P. ), pp. 73-119. Boca Raton: CRC Press.
von der Mark, K., Kirsch, T., Nerlich, A., Kuss, A., Weseloh, G., Gluckert, K. & Stoss, H. (1992) Type X collagen synthesis in human osteoarthritic cartilage. Arthritis Rheum. 35, 806-11.
von der Mark, K., Frischholz, S., Aigner, T., Beier, F., Belke, J., Erdmann, S. & Burkhardt, H. (1995) Upregulation of type X collagen expression in osteoarthritic cartilage. Acta Othop. Scand. (suppl. 266) 66, 125-9.
Walker, G.D., Fischer, M., Gannon, J., Thompson, R.C. & Oegema, T.R. (1995) Expression of type X collagen in osteoarthritis. J. Orthop. Res. 13, 4-12.
Welgus, H.G., Fliszar, C. J., Seltzer, J.L., Schmid, T.M. & Jeffrey, J. J. (1990) Differential susceptibility of type X collagen to cleavage by two mammalian interstitial collagenases and 72-kDa type IV collagenase. J. Biol. Chem. 265, 13521-7.
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Roberts, S., Bains, M.A., Kwan, A. et al. Type X collagen in the human invertebral disc: an indication of repair or remodelling?. Histochem J 30, 89–95 (1998). https://doi.org/10.1023/A:1003278915981
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DOI: https://doi.org/10.1023/A:1003278915981