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Immunolocalization of myotonic dystrophy protein kinase in corbular and junctional sarcoplasmic reticulum of human cardiac muscle

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Abstract

The subcellular localization of myotonic dystrophy protein kinase has been examined in human cardiac muscles with confocal laser-scanning microscopy and electron microscopy. A polyclonal antibody was produced against the synthesized peptide from a human kinase cDNA clone. We checked the antibody specificity for cardiac myotonic dystrophy protein kinase using an immunoblotting technique. Immunoblotting of extract from human cardiac muscles showed mainly 70 kDa and 55 kDa molecular weight bands. Confocal images of the protein kinase immunostaining showed striated banding patterns similar to those of skeletal muscles. In addition, the kinase was strongly detected around the intercalated disc. Immunoelectron microscopy showed that the kinase was mainly expressed in both corbular and junctional sarcoplasmic reticulum, but not in network sarcoplasmic reticulum. These results suggest that myotonic dystrophy protein kinase may be involved in the modulation of Ca2+ homeostasis in cardiac myofibres. © 1998 Chapman & Hall

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Authors and Affiliations

  1. Department of Anatomy, Yamanashi Medical University, Shimokato Yamanashi, Japan

    HIDEHO UEDA, TAKESHI BABA, NOBUO TERADA & SHINICHI OHNO

  2. Department of Neurology, Tokyo Medical and Dental University School of Medicine, Tokyo, Japan

    NORIYOSHI KAMEDA, MASATAKE SHIMOKAWA, MASAHIKO YAMAMOTO & TAKAYOSHI KOBAYASHI

  3. Institute of Molecular and Cellular Biosciences, University of Tokyo, Tokyo, Japan

    SHOICHI ISHIURA

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  1. HIDEHO UEDA
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UEDA, H., KAMEDA, N., BABA, T. et al. Immunolocalization of myotonic dystrophy protein kinase in corbular and junctional sarcoplasmic reticulum of human cardiac muscle. Histochem J 30, 245–251 (1998). https://doi.org/10.1023/A:1003207822341

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