Abstract
Recent research has indicated that the hatchability of mass-producedrotifer (Brachionus plicatilis) resting eggs (stored in sea water in thedark at 4°C) gradually decreases due to bacterialinfection of the porous egg surface. We investigated whether lyophilizationas well as a canning treatment could solve the problem of bacterialinfection of resting eggs. The canning method preserves eggs in a dry form,after lyophilization at −40°C. The resting eggs werepacked in cans under various pressures: 21, 34, 48, 61, 75, 88 and 101 kPa.The viability of the canned resting eggs was tested by hatching experimentsdone 6 and 12 months after canning and storage at 5°C.When partial pressures were less than 88 kPa, percent hatching after 6months ranged between 16 and 20%, similar to that of uncanned eggs(19% hatching), but significantly lower (9 and 11%, P <0.01) at 88 and 101 kpa. Even after 12 months, percent hatching remained at35% when canning was at 61 kpa. Lyophilization and canning at partialpressure lower than 88 kPa could therefore preserve resting eggs, andhatchability was maintained. Canned eggs that were treated with NaClO (1 mgl−1) or NFS-Na (5 mg l−1) showed a significantlyhigher percent hatching (68% and 80%, respectively, each P<0.05) than untreated eggs.
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Balompapueng, M. D., N. Munuswamy, A. Hagiwara & K. Hirayama. Effect of disinfectants on the hatching of marine rotifer resting eggs (Brachionus plicatilisMüller). Aquacult. Res. 28: 559–565.
Balompapueng, M. D., A. Hagiwara, A. Nishi, K. Imaizumi & K. Hirayama, 1997. Resting egg formation of the rotifer Brachionus plicatilisusing a semicontinuous culture method. Fish. Sci.
Hagiwara, A., 1994. Practical use of rotifer cysts. Israel. J.Aquacult.-Bamidgeh 46: 13–21.
Hagiwara, A., 1996. Appearance of floating resting eggs in the rotifers Brachionus plicatilisand B. rotundiformis. Bull. Fac. Fish. Nagasaki Univ. 77: 111–115.
Hagiwara, A. & A. Hino, 1989. Effect of incubation and preservation on resting egg hatching and mixis in the derived clones of the rotifer Brachionus plicatilis. Hydrobiologia 186/187: 415–421.
Hagiwara, A. & K. Hirayama, 1993. Preservation of rotifers and its application in the finfish hatchery. In C.-S. Lee, M. S. Su & I. C. Liao (eds), Finfish Hatchery in Asia: Proc. Finfish Hatchery in Asia ’91. TML Conf. Proc. 3: 61–71, Tungakang Marine Lab., Taiwan Fisheries Research Inst., Tungkang, Pingtung, Taiwan.
Hagiwara, A., M. D. Balompapueng, N. Munuswamy & K. Hirayama, 1996. Mass production of the marine rotifer resting eggs. Aquaculture 155: 223–230.
Hagiwara, A., K. Hamada, A. Nishi, K. Imaizumi & K. Hirayama, 1993a. Mass production of rotifer resting eggs in 50m3 tanks. Nippon Suisan Gakkaishi 59: 93–98.
Hagiwara, A., K. Hamada, A. Nishi, K. Imaizumi & K. Hirayama, 1993b. Dietary value of neonates from rotifer Brachionus plicatilis resting eggs for red sea bream larvae. Nippon Suisan Gakkaishi 59: 99–104.
Hagiwara, A., N. Hoshi, F. Kawahara, T. Tominaga & K. Hirayama, 1995. Resting eggs of the marine rotifer Brachionus plicatilis Muller: development, and effect of irradiation on hatching. Hydrobiologia 313/314: 223–229.
Hino, A., 1993. Present culture systems of the rotifer Brachionus plicatilis and the function ofmicroorganisms. In C.S. Lee, M. S. Su & J. C. Liao (eds). Finfish Hatchery in Asia: Proc. Finfish Hatchery in Asia ’91. TML Conf. Proc. 3: 51–59, Tungakang Marine Lab., Taiwan Fisheries Research Inst., Tungkang, Pingtung, Taiwan.
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Balompapueng, M.D., Hagiwara, A., Nozaki, Y. et al. Preservation of resting eggs of the euryhaline rotifer Brachionus plicatilis O. F. Müller by canning. Hydrobiologia 358, 163–166 (1997). https://doi.org/10.1023/A:1003197222440
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DOI: https://doi.org/10.1023/A:1003197222440