The activity of the degradation products of linoleic and linolenic acids in inhibiting the growth of the green alga Chlorella pyrenoidosa was determined using the paper disk-agar plate method. Although hydroperoxides derived from these acids and aldehyde biodegradation products and other related aldehydes and alcohols showed inhibitory activity, it is concluded from the weakness of the activities that the inhibitory effects of polyunsaturated fatty acids(PUFAs) on Chlorella growth is due mainly to the acids themselves and not their degradation products. The evidence for the possible ecological role of PUFAs as toxic agents against phytoplankton is presented.
This is a preview of subscription content, access via your institution.
Buy single article
Instant access to the full article PDF.
Tax calculation will be finalised during checkout.
Subscribe to journal
Immediate online access to all issues from 2019. Subscription will auto renew annually.
Tax calculation will be finalised during checkout.
Arzul, G., P. Gentien, G. Bodennec, F. Toularastel, A. Yarenou & M.P. Crassous, 1995. Comparison of toxic effects of Gymnodinium cf. nagasakiensepolyunsaturated fatty acids. In P. Lassus, G. Arzul, E. Erard-Le Denn, P. Gentien & C. Marcaillou-Le Baut (eds), Harmful Marine Algal Blooms. Lavoisier Publishing, Paris, France: 395–400.
Galliard, T. & H.W.-S. Chan, 1980. Lipoxygenases. In P.K. Stumpf (ed.), Biochemistry of plants, Vol. 4, Lipids-Structure and function. Academic Press, New York, NY, U.S.A.: 131–161.
Galliard, T., D.R. Phillips & J. Reynolds, 1976. The formation of cis-3-nonenol, trans-2-nonenal, and hexanal from linoceic acid hydroperoxide isomers by a hydroperoxide cleavage enzyme system in cucumber fruits. Biochim. Biophys. Acta 441: 181–192.
Gardner, H.W., 1991. Recent investigations into the lipoxygenase pathway in plants. Biochim. Biophys. Acta 1084: 221–239.
Garssen, G.J., J.F.G. Vliegenthart & J. Boldingh, 1972. The origin and structures of dimeric fatty acids from the anaerobic reaction between soya-bean lipoxygenase, linoleic acid and its hydroperoxide. Biochem. J. 130: 435–442.
Hamberg, M. & B. Samuelsson, 1967. On the specificity of the oxygenation of unsaturated fatty acids catalyzed by soybean lipoxidase. J. Biol. Chem. 242: 5329–5335.
Ikawa, M., C. Carr & T. Tatsuno, 1985. Trichothecene structure and toxicity to the green alga Chlorella pyrenoidosa. Toxicon 23: 535–537.
Ikawa, M., J.F. Haney & J.J. Sasner, 1996. Inhibition of Chlorella Growth by the lipids of cyanobacterium Microcystis aeruginosa. Hydrobiologia 331: 167–170.
Ikawa, M., T. Hartshorne, L.-A. Caron, R.C. Iannitelli, L.J. Barbero & K. Wegener, 1984. Inhibition of growth of the green alga Chlorella pyrenoidosaby unsaturated fatty acids. J. am. Oil. Chemists’ Soc. 61: 1877–1878.
Ikawa, M., S.P. Mosley & L.J. Barbero, 1992. Inhibitory effects of terpene alcohols and aldehydes on growth of green alga Chlorella pyrenoidosa. J. Chem. Ecol. 18: 1755–1759.
Ikawa, M., J.J. Sasner & J.F. Haney, 1994. Lipids of cyanobacterium Aphanizomenon flos-aquaeand inhibition of Chlorellagrowth. J. Chem. Ecol. 20: 2429–2436.
Lam, C.W.Y. & W.B. Silvester, 1979. Growth interactions among blue-green (Anabaena oscillarioides, Microcystis aeruginosa) and green (Chlorella sp.) algae. Hydrobiologia 63: 135–143.
McCracken, M.D., R.D. Middaugh & R.S. Middaugh, 1980. A chemical characterization of an algal inhibitor obtained from Chlamydomonas. Hydrobiologia 70: 271–276.
Matthew, J.A. & T. Galliard, 1978. Enzymic formation of carbonyls from linoleic acid in leaves of Phaseolus vulgaris. Phytochemistry 17: 1043–1044.
Mead, J.F., R.B. Alfin-Slater, D.R. Howton & G. Popjak, 1986. Lipids: Chemistry, Biochemistry, and Nutrition, Plenum, New York, NY, U.S.A.: 83–99.
Porter, N.A., B.A. Weber, H. Weener & J.A. Khan, 1980. Autoxidation of polyunsaturated lipids. Factors controlling the stereochemistry of product hydrocarbons. J. am. Chem. Soc. 102: 5597–5601.
Proctor, V.W., 1957. Studies of algal antibiosis using Haematococcus and Chlamydomonas. Limnol. Oceanogr. 2: 125–139.
Scutt, J.E., 1964. Autoinhibitor production by Chlorella vulgaris. Am. J. Bot. 51: 581–584.
Vick, B.A. & D.C. Zimmerman, 1987. Oxidative systems for modi-fication of fatty acids: The lipoxygenase pathway. In P.K. Stumpf (ed.), The Biochemistry of Plants, Vol. 9, Lipids: Structure and Function, Academic Press, Orlando, Florida, U.S.A.: 53–90.
Vick, B.A. & D.C. Zimmerman, 1989. Metabolism of fatty acid hydroperoxides by Chlorella pyrenoidosa. Plant Physiol. 90: 125–132.
Yamada, N., N. Murakami, T. Morimoto & J. Sakakibara, 1993. Auto-growth inhibitory substance fromthe fresh-water cyanobacterium Phormidium tenue. Chem. Pharm. Bull. 41: 1863–1865.
About this article
Cite this article
Ikawa, M., Sasner, J.J. & Haney, J.F. Inhibition of Chlorella growth by degradation and related products of linoleic and linolenic acids and the possible significance of polyunsaturated fatty acids in phytoplankton ecology. Hydrobiologia 356, 143–148 (1997). https://doi.org/10.1023/A:1003103726520
- polyunsaturated fatty acids
- linoleic acid
- linolenic acid
- Chlorella pyrenoidosa
- growth inhibition