Abstract
Cell suspension cultures of ‘Peter Pears’, a cultivar of Gladiolus x grandiflorus (Hort.), susceptible to the fungus Fusarium oxysporum f. sp. gladioli (Mass.), have been challenged with fusaric acid, one of the toxins produced by this pathogen. Selected cell-lines showed increased tolerance to the toxin and grew even on concentrations of fusaric acid up to 0.5 mm. When inoculated with a conidial suspension, the mycelial growth on selected cell-lines was limited compared to the control. Fusaric acid greatly reduced plant regeneration, and only two plants were obtained from the callus subcultured for a prolonged time on medium with fusaric acid. These plants have a significantly altered DNA content compared to the control. A reduced callus phase on toxin-containing medium, resulted in an improved regeneration and the DNA content of the regenerated plantlets was similar to the control. Some plants regenerated from these fusaric acid-insensitive cell-lines showed an increased tolerance to the toxin when cultured in vitro in presence of fusaric acid. The selected plants will be further tested for Fusarium-resistance once maturity is reached.
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Remotti, P.C., Löffler, H.J. & van Vloten-Doting, L. Selection of cell-lines and regeneration of plants resistant to fusaric acid from Gladiolus × grandiflorus cv. ‘Peter Pears’. Euphytica 96, 237–245 (1997). https://doi.org/10.1023/A:1003034215722
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DOI: https://doi.org/10.1023/A:1003034215722