Skip to main content
Log in

A simple method for the evaluation of red clover (Trifolium pratense L.) resistance to Sclerotinia trifoliorum

  • Published:
Euphytica Aims and scope Submit manuscript

Abstract

Sclerotinia crown and stem rot is the most destructive disease on red clover in areas with cool seasons including North America and Europe. An artificial inoculation test in controlled conditions has been developed in order to evaluate resistance to this fungus in individual plants. Measured drops of ascospore suspension were applied to detached clover leaflets and disease severity scored on a visual scale. Clovers from 16 cultivars and 12 ecotypes were tested for resistance to Sclerotinia trifoliorum. The results obtained for cultivar resistance are in agreement with those of other tests described in the literature. Moreover, repeated individual evaluations on detached leaflets from the same plant was successful in detecting genotypic variation within clover populations.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Arseniuk, E., 1989. Effect of induced autotetraploidy on response to Sclerotinia clover rot in Trifolium pratense L. Plant Breeding 103: 310–318.

    Google Scholar 

  • Castaño, F., F. Vear & D. Tourvieille de Labrouhe, 1989. Utilisation de plusieurs tests simultanés dans la sélection pour la résistance du tournesol vis-à-vis de Sclerotinia sclerotiorum. Informations techniques du CETIOM 107: 14–20.

    Google Scholar 

  • Delclos, B. & G. Raynal, 1995. Comparison of techniques for the production of Sclerotinia trifoliorum ascospores in the laboratory for forage legumes resistance tests. Journal of Phytopathology 143: 345–348.

    Google Scholar 

  • Dijkstra, J., 1964. Inoculation with ascospores of Sclerotinia trifoliorum for detection of clover rot resistant red clover. Euphytica 13: 314–329.

    Google Scholar 

  • Dixon, G. R. & J. K. Doodson, 1974. Techniques for testing the resistance of red clover cultivars to Sclerotinia trifoliorum Eriks. (clover rot). Euphytica 23: 671–679.

    Google Scholar 

  • Loveless, A. R., 1951. Observation on the biology of clover rot. Ann. Appl. Biol 38: 642–665.

    Google Scholar 

  • Marum, P., R. R. Smith & C. R. Grau, 1994. Development of procedures to identify red clover resistant to Sclerotinia trifoliorum. Euphytica 77: 257–261.

    Google Scholar 

  • Pratt, R. G. & D.E. Rowe, 1991. Differential responses of Alfalfa genotypes to stem inoculations with Sclerotinia sclerotiorum and S. trifoliorum. Plant Disease 75: 188–191.

    Google Scholar 

  • Raynal, G., 1981a. La sclérotiniose des trèfles et luzernes à Sclerotinia trifoliorum Eriks. I. — Choix d'une méthode de inoculation artificielle. Agronomie 1: 565–572.

    Google Scholar 

  • Raynal, G., 1981b. La sclerotiniose des trèfles et luzernes à Sclerotinia trifoliorum Eriks. II. — Variabilité du parasite, résistance des plantes en conditions contrôlées. Agronomie 1: 573–578.

    Google Scholar 

  • Raynal, G., 1983. Production au laboratoire d'apothécies de Sclerotinia trifoliorum Eriks. pour l'évaluation de la résistance du trèfle violet à la sclérotiniose. Agronomie 3: 369–373.

    Google Scholar 

  • Raynal, G., 1985. Observations sur le comportement variétal au champ du trèfle violet vis-à-vis de la sclérotiniose et sur l'épidémiologie de la maladie. Fourrages 101: 85–104.

    Google Scholar 

  • Raynal, G., P. Gayraud, C. Mousset-Déclas & A. Serpeille, 1991. Possibilités de lutte contre la sclérotiniose du trèfle violet. Fourrages 127: 335–344.

    Google Scholar 

  • Rowe, D. E. & R. E. Welty, 1984. Indirect selection for resistance to Sclerotinia crown and stem rot on alfalfa. Can. J. Plant Sci. 64: 145–150.

    Google Scholar 

  • Schmidt, D., 1980. Etude sur la résistance du trèfle violet à Sclerotinia trifoliorum Eriks. Revue Suisse Agric. 12: 197–206.

    Google Scholar 

  • Scott, S.W., 1981. Separation of Sclerotinia isolates collected from three herbage legume hosts. Br. Mycol. Soc. 76: 321–323.

    Google Scholar 

  • Tourvieille de Labrouhe, D. & F. Vear, 1990. Heredity of resistance to Sclerotinia sclerotiorum in sunflowers. III. — Study of reactions to artificial infections of roots and cotyledons. Agronomie 10: 323–330.

    Google Scholar 

  • Valleau, W. D., E. N. Fergus & L. Henson, 1933. Resistance of red clovers to Sclerotinia trifoliorum Erik., and infection studies. Kentucky Agric. Exp. Stat. Bull. 341: 113–131.

    Google Scholar 

  • Vear, F. & D. Tourvieille de Labrouhe, 1988. Heredity of resistance to Sclerotinia sclerotiorum in sunflowers. II. — Study of capitulum resistance to natural and artificial ascospore infections. Agronomie 8: 503–508.

    Google Scholar 

  • Vestad, R., 1960. The effect of induced autotetraploidy on resistance to clover rot (Sclerotinia trifoliorum Erikss.) in red clover. Euphytica 9: 35–38.

    Google Scholar 

  • Yamaguchi, H., A. Sawai & K. Uchiyama, 1993. Improvement of red clover persistence in Japan. Proc. XVII Intern. Grassl. Cong., Feb. 8–21 1993, pp. 666–667.

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Delclos, B., Mousset-Déclas, C. & Raynal, G. A simple method for the evaluation of red clover (Trifolium pratense L.) resistance to Sclerotinia trifoliorum. Euphytica 93, 173–179 (1997). https://doi.org/10.1023/A:1002914002775

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1002914002775

Navigation