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Low-Molecular-Weight Glycoprotein from Cattle Blood Serum: Structure and Properties

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Abstract

The amino acid composition, structure, and physicochemical properties of a low-molecular-weight glycoprotein from cattle blood serum (SGP) were studied. The content of carbohydrates (represented by mannose-rich oligosaccharides) amounted to 45–50 wt %. The value of the specific partial heat of SGP, measured by differential scanning calorimetry (DSC), equaled 1.8 J/(g K), which is characteristic of unfolded proteins. Circular dichroic (CD) spectra of SGP led us to conclude that it is not highly structured and that it occurs in the shape of a statistical globule. The protein was deglycated using anhydrous trifluoromethane sulfonate (TFMS), after which its amino acid composition and the sequence of a fragment were determined. The results indicate that SGP is a protein not studied previously.

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Authors and Affiliations

  1. Nesmeyanov Institute of Organoelemental Compounds, Russian Academy of Sciences, ul. Vavilova 28, Moscow, GSP-1, 117813, Russia

    I. A. Yamskov, A. A. Vinogradov & L. A. Maslova

  2. Emanuel Institute of Biochemical Physics, Russian Academy of Sciences, ul. Kosygina 4, Moscow, 117977, Russia

    A. N. Danilenko

  3. Kol'tsov Institute of Developmental Biology, Russian Academy of Sciences, ul. Vavilova 26, Moscow, 117808, Russia

    E. Yu. Rybakova & V. P. Yamskova

Authors
  1. I. A. Yamskov
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  2. A. A. Vinogradov
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  3. A. N. Danilenko
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  4. L. A. Maslova
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  5. E. Yu. Rybakova
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  6. V. P. Yamskova
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Yamskov, I.A., Vinogradov, A.A., Danilenko, A.N. et al. Low-Molecular-Weight Glycoprotein from Cattle Blood Serum: Structure and Properties. Applied Biochemistry and Microbiology 37, 29–35 (2001). https://doi.org/10.1023/A:1002884106796

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