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The Substrate Preference and Histochemical Localization Argue against the Direct Role of Cucumber Stress-Related Anionic Peroxidase in Lignification

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Biologia Plantarum

Abstract

The substrate preference and the localization of cucumber (Cucumis sativus L.) stress-related anionic peroxidase (srPRX) were investigated in order to assess whether this activity correlates with the lignification. The results showed that none of the purified srPRX isoenzymes (PRX 1 –3) could oxidize the lignin monomer analog syringaldazine. The srPRX immunospecific signal was found to be highly abundant in both the extrafascicular and fascicular phloem regions in cucumber stem and leaf petiole. In Nicotiana, Petunia and Dahlia, the srPRX homologs were specifically deposited in both outer and inner phloem elements of stem and in both abaxial and adaxial phloem of leaf stems. The srPRX mRNA expression analysis showed similar pattern as for immunolocalization. The subcellular localization of immunospecific srPRX demonstrated that at least part of the peroxidase could be ionically-bound to phloem cell wall.

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Authors and Affiliations

  1. Laboratory of Molecular Biology and Virology, Research Institute of Viticulture and Enology, (C.R.I.V.E.), Matúškova 25, SK-83311, Bratislava, Slovakia e-mail

    V. Repka, D. Štetková & I. Fischerová

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  1. V. Repka
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  2. D. Štetková
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  3. I. Fischerová
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Correspondence to V. Repka.

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Repka, V., Štetková, D. & Fischerová, I. The Substrate Preference and Histochemical Localization Argue against the Direct Role of Cucumber Stress-Related Anionic Peroxidase in Lignification. Biologia Plantarum 43, 549–558 (2000). https://doi.org/10.1023/A:1002813530741

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