Abstract
The effects of water stress on the contents of proline, ornithine, arginine and glutamic acid in detached rice leaves were examined. In water stressed leaves, the content of proline was elevated to a content approximately 8-, 14- and 17-fold higher than in control leaves after 4, 8 and 12 h, respectively. We also observed that omithine and arginine contents were much higher under water stress than in control leaves. However, the content of glutamic acid in water stressed leaves was higher after 4 and 8 h and lower after 12 h than that in control leaves.
References
Bradford, M.M.: A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principles of protein-dye binding.-Anal. Biochem. 72: 248–254, 1976.
Brown, D.H., Fowden, D.: Metabolism of delta-acetylornithine in two leguminous species.-Phytochemistry 5: 887–892, 1966.
Chiang, H.-H., Dandekar, A.M.: Regulation of proline accumulation in Arabidopsis thaliana (L.) Heynh. during development and in response to desiccation.-Plant Cell Environ. 18: 1280–1290, 1995.
Delauney, A., Hu, C., Kavi Kishor, P.B., Verma, D.: Cloning of ornithine-δ-aminotransferase cDNA from Vigna aconitifolis by trans-complementation in Escherichia coli and regulation of proline biosynthesis.-J. biol. Chem. 268: 18673–18678, 1993.
Kao, C.H.: Senescence of rice leaves VI. Comparative study of the metabolic changes of senescing turgid and water stressed excised leaves.-Plant Cell Physiol. 22: 683–688, 1981.
Mattioni, C., Lacerenza, N.G., Troccoli, A., De Leonardis, A.M., Di Fonzo, N.: Water and salt stress-induced alterations in proline metabolism of Triticum durum seedlings.-Physiol. Plant. 101: 787–792, 1997.
Mukherjee, S.P., Choudhuri, M.A.: Implications of water stress-induced changes in the levels of endogenous ascorbic acid hydrogen peroxide in Vigna seedlings.-Physiol. Plant. 58: 166–170, 1983.
Munns, R., Brady, C.J., Barlow, E.W.: Solute accumulation in the apex and leaves of wheat during water stress.-Aust. J. Plant Physiol. 6: 379–389, 1979.
Roosens, N.H.C.J., Thu, T.T., Iskander, H.M., Jacobs, M.: Isolation of the ornithine-δ-aminotransferase cDNA and effect of salt stress on its expression in Arabidopsis thaliana.-Plant Physiol. 117: 263–271, 1988.
Venekamp, J.H., Kost, J.T.M.: The sources of free proline and asparagine in field bean plants, Vicia faba L., during and after a short period of water withholding.-J. Plant Physiol. 132: 102–109, 1988.
Yang, C.-W., Kao, C.H.: Importance of ornithine-δ-aminotransferase to proline accumulation caused by water stress in detached rice leaves.-Plant Growth Regul. 27: 189–192, 1999.
Yang, C.-W., Lin, C.C., Kao, C.H.: Endogenous ornithine and arginine contents and dark-induced proline accumulation in detached rice leaves.-J. Plant Physiol., in press, 2000.
Yang, C.-W., Wang, J.W., Kao, C.H.: The relation between accumulation of abscisic acid and proline in detached rice leaves.-Biol. Plant. 43: 301–304, 2000.
Yoshiba, K., Kiyosue, T., Nakashima, K., Yamaguchi-Shinozaki, K., Shinozaki, K.: Regulation of levels of proline as an osmolyte in plants under water stress.-Plant Cell Physiol. 38: 1095–1102, 1997.
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Yang, CW., Lin, C. & Kao, C. Proline, Ornithine, Arginine and Glutamic Acid Contents in Detached Rice Leaves. Biologia Plantarum 43, 305–307 (2000). https://doi.org/10.1023/A:1002733117506
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DOI: https://doi.org/10.1023/A:1002733117506