An unknown yeast species was isolated from maize silage and was determined to be novel on the basis of morphological and physiological characteristics, nucleotide sequence of domain D1/D2 of LSU rDNA and from its electrophoretic karyotype. The name for the proposed new species is Saccharomyces bulderi Middelhoven, Kurtzman et Vaughan-Martini (type strain CBS 8638, NRRL Y-27203, DBVPG 7127). S. bulderi is closely related to S. barnettii and S. exiguus from which it can be distinguished by having a double vitamin requirement of biotin and thiamine and by no or slow aerobic growth on raffinose, a sugar that on the contrary is fermented rapidly. Gluconolactone is rapidly fermented with ethanol, glycerol and carbon dioxide being the main products.
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Bulder CJEA (1963) On respiratory deficiency in yeasts. PhD Thesis, Delft
Bulder CJEA (1964) Lethality of the petite mutation in petite negative yeasts. Antonie van Leeuwenhoek 30: 442–454
Bulder CJEA (1966) Lethality in respiratory deficiency and utilization of fermentation energy in petite negative yeasts. Arch f Mikrobiol. 53: 189–194
Kluyver AJ (1914) Biochemische suikerbepalingen. PhD Thesis, Delft
Kurtzman CP & Robnett CJ (1998) Identification and phylogeny of ascomycetous yeasts from analysis of nuclear large subunit (26S) ribosomal DNA partial sequences. Antonie van Leeuwenhoek 73: 331–371
Marmur J & Doty P (1962) Determination of the base composition of desoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol. 5: 109–118
Middelhoven WJ (1997) Assimilation of organic acids: the pH as determining factor. YEAST, a newsletter for persons interested in yeast 46(II): 19–20
Middelhoven WJ & Franzen MM (1986) The yeast flora of ensiled whole-crop maize. J Sci Food Agric. 37: 855–861
Vaughan-Martini A (1995) Saccharomyces barnetti and Saccharomyces spencerorum: two new species of Saccharomyces sensu lato (van der Walt). Antonie van Leeuwenhoek 68: 111–118
Vaughan-Martini A & Martini A (1998) Saccharomyces Meyen ex Reess. In: Kurtzman CP & Fell JW (Eds) The Yeasts, a taxonomic study, 4th edn. (pp 358–371). Elsevier, Amsterdam
van derWalt JP & Yarrow D (1984) Methods for the isolation, maintenance, classification and identification of yeasts. In: Kreger-van Rij NJW (Ed) The Yeasts, a taxonomic study, 3rd edn. (pp 45–104). Elsevier Science Publishers, Amsterdam
van Hoek P, van Dijken JP & Pronk JT (1998) Effect of specific growth rate on fermentative capacity of baker's yeast. Appl Environm Microbiol. 64: 4226–4233
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Middelhoven, W.J., Kurtzman, C.P. & Vaughan-Martini, A. Saccharomyces bulderi sp. nov., a yeast that ferments gluconolactone. Antonie Van Leeuwenhoek 77, 223–228 (2000). https://doi.org/10.1023/A:1002414301967
- Saccharomyces bulderi