Abstract
The shoot apices of ten groundnut interspecific hybrids, showing peanut stripe virus symptoms were used as explants. Murashige and Skoog (MS) medium containing 1 mg dm-3 each of naphthaleneacetic acid (NAA) and benzylaminopurine (BAP) supported maximum callusing of the hybrids J 11 × Arachis sp. PI 30008 as well as J 11 × A. sp. Manfredi # 5 (86.3 and 82.6 %, respectively). On MS medium with 3.5 mg dm-3 NAA and 0.2 mg dm-3 BAP, the meristems of J 11 × A. stenosperma as well as J 11 × A. otavioi gave good response (79.7 and 77.2 %). The regeneration frequencies ranged from 16.7 to 76.9 % and was more than 30 % in all hybrids except for J 11 × PI 30085. Shoots (5 cm) regenerated from the virus-free (ELISA tested) calli rooted well (60 – 80 %) on MS basal medium supplemented with 0.4 % activated charcoal and 200 mg dm-3 casein hydrolysate. In addition, long shoots which failed to produce roots, when transferred to soil during rainy season and protected from direct sun got established.
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Radhakrishnan, T., Murthy, T., Desai, S. et al. Meristem Culture of Interspecific Hybrids of Groundnut. Biologia Plantarum 42, 309–312 (1999). https://doi.org/10.1023/A:1002181223243
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DOI: https://doi.org/10.1023/A:1002181223243