Abstract
Sunflower (Helianthus annuus L.) seeds were germinated and grown in the presence of 50, 100 and 200 µM CdCl2. The lower concentration (50 µM) of Cd2 ions produced slight decrease in reduced glutathione (GSH) content and overall increase (except superoxide dismutase) in antioxidant enzyme activities, and in H2O2 concentration. Chlorophyll content, lipid peroxidation and protein oxidation were not affected under 50 µM CdCl2. GSH content was diminished under 100 and 200 µM CdCl2, and except for superoxide dismutase, which activity remained unaltered, overall decreases in the antioxidant enzyme activities (catalase, ascorbate peroxidase, dehydroascorbate peroxidase, glutathione reductase) and in guaiacol peroxidase were observed. These Cd2 concentrations caused a decrease in chlorophyll content as well as an increase in lipid peroxidation, protein oxidation and H2O2 concentration. All the observed effects were more evident with the highest concentration of cadmium chloride used.
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References
Anderson, M.E.: Determination of glutathione and glutathione disulfide in biological samples.-Methods Enzymol. 113: 545–548, 1985.
Bailly, C., Benamar, A., Corbineau, F., Come, D.: Changes in malondialdehyde content and in superoxide dismutase, catalase and glutathione reductase activities in sunflower seeds as related to deterioration during accelerated ageing.-Physiol. Plant. 97: 104–110, 1996.
Becana, M., Aparicio-Tejo, P., Irigoyen, J.J., Sánchez-Díaz, M.: Some enzymes of hydrogen peroxide metabolism in leaves and root nodules of Medicago sativa.-Plant Physiol. 82: 1169–1171, 1986.
Bradford, M.M.: A rapid and sensitive method for the quantification of microgram quantities of proteins utilizing the principle of protein-dye binding.-Anal. Biochem. 72: 248–254, 1956.
Chance, B., Sies, H., Boveris, A.: Hydroperoxide metabolism in mammalian organs.-Physiol. Rev. 59: 527–605, 1979.
Dalton, D., Hanus, F.J., Russel, S.A., Evans, H.J.: Purification, properties, and distribution of ascorbate peroxidase in legume root nodules.-Plant Physiol. 83: 789–794, 1987.
Esterbauer, H., Grill, D.: Seasonal variation of glutathione and glutathione reductase in needles of Picea abies.-Plant Physiol. 61: 119–121, 1978.
Gallego, S.M., Benavides, M.P., Tomaro, M.L.: Effect of heavy metal ion excess on sunflower leaves: evidence for involvement of oxidative stress.-Plant Sci. 121: 151–159, 1996.
Guilbault, G.G., Brignac, P.J., Juneau, M.: New substrates for the fluorometric determination of oxidative enzymes.-Anal. Chem. 40: 1256–1263, 1968.
Hendry, G.A.F., Baker, A.J.M., Ewart, C.F.: Cadmium tolerance and toxicity, oxygen radical processes and molecular damage in cadmium-tolerant and cadmium-sensitive clones of Holcus lanatus.-Acta bot. neerl. 41: 271–281, 1992.
Levine, R.L., Garland, D., Oliver, C.N., Amici, A., Climent, I., Lenz, A., Ahn, B., Shaltiel, S., Stadtman, E.R.: Determination of carbonyl content in oxidatively modified proteins.-Methods Enzymol. 106: 464–478, 1990.
Luna, C.M., González, C.A., Trippi, V.S.: Oxidative damage caused by an excess of copper in oat leaves.-Plant Cell Physiol. 35: 11–15, 1994.
Minotti, G., Aust, S.D.: The requirements of iron(III) in the initiation of lipid peroxidation by iron (II) and hydrogen peroxide.-J. biol. Chem. 262: 1098–1104, 1987.
Nakano, Y., Asada, K.: Hydrogen peroxide is scavenged by ascorbate-specific peroxidase in spinach chloroplast.-Plant Cell Physiol. 22: 867–880, 1981.
Pastori, G.M., Trippi, V.S.: Oxidative stress induces high rate of glutathione reductase synthesis in a drought-resistant maize strain.-Plant Cell Physiol. 33: 957–966, 1992.
Salin, M.L.: Toxic oxygen species and protective systems of the chloroplast.-Physiol. Plant. 72: 681–689, 1987.
Salt, D.E., Blaylock, M., Kumar, N.P.B.A., Dushenkov, V., Ensley, B.D., Chet, I., Raskin, I.: Phytoremediation: a novel strategy for the removal of toxic metals from the environment using plants.-Biotechnology 13: 468–474, 1995.
Scandalios, J.G.: Oxygen stress and superoxide dismutases.-Plant Physiol. 101: 7–12, 1993.
Shaedle, M., Bassham, J.A.: Chloroplast glutathione reductase.-Plant Physiol. 59: 1011–1012, 1977.
Somashekaraiah, B.V., Padmaja, K., Prasad, A.R.K.: Phytotoxicity of cadmium ions on germinating seedlings of mung bean (Phaseolus vulgaris): Involvement of lipid peroxides in chlorophyll degradation.-Physiol. Plant. 85: 85–89, 1992.
Stohs, S.J., Bagchi, D.: Oxidative mechanisms in the toxicity of metal ions.-Free Radicals Biol. Med. 18: 321–336, 1995.
Stroinski, A., Kozlowska, M.: Cadmium-induced oxidative stress in potato tuber.-Acta Soc. Bot. Pol. 66: 189–195, 1997.
Stroinski, A., Zielezinska, M.: Cadmium effect on hydrogen peroxide, glutathione and phytochelatins levels in potato tuber.-Acta Physiol. Plant. 19: 127–135, 1997.
Van Assche, F., Clijsters, H.: Effects of metals on enzyme activity in plants.-Plant Cell Environ. 13: 195–206, 1990.
Wintermans, J.F., De Mots, A.: Spectrophotometric characteristics of chlorophylls a and b and their pheophytins in ethanol.-Biochim biophys. Acta 109: 448–453, 1965.
Zhang, J., Kirkham, M.B.: Enzymatic responses of the ascorbate-glutathione cycle to drought in sorghum and sunflower plants.-Plant Sci. 113: 139–147, 1996.
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Gallego, S., Benavides, M. & Tomaro, M. Effect of Cadmium Ions on Antioxidant Defense System in Sunflower Cotyledons. Biologia Plantarum 42, 49–55 (1999). https://doi.org/10.1023/A:1002159123727
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DOI: https://doi.org/10.1023/A:1002159123727