Abstract
The changes in the autofluorescence spectra of intact Philadelphus grandiflorus, Epiphyllum hybridum, and Plantago major pollen have been observed with microspectrofluorometric method after 100-h exposure to ozone (3 h per day for 5 d per week in total dose 5.0 cm3 m-3). The fluorescence maximum at 530 - 550 nm disappeared in carotenoid-containing pollen of Philadelphus grandiflorus and Epiphyllum hybridum and new maximum at 475 - 480 nm arose that correlated with lipofuscin-like substances observed in the extracts from the pollen grains. The carotenoid-less pollen of Plantago major showed only the increase of the maximum at 470 nm, and no lipofuscin in the extracts. It is supposed that main indicator of the pollen damage by ozone in carotenoid-containing microspores are the lipofuscin-like compounds.
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References
Brooks, R.I., Csallany, A.S.: Effects of air, ozone, and nitrogen dioxide exposure on the oxidation of corn and soybean lipids.-J. Agr. Food Chem. 28: 1203–1209, 1978.
Bradford, M.M.: A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.-Anal. Biochem. 72: 248–254, 1976.
Feder, W.A.: Reduction in tobacco pollen germination and tube elongation, induced by low levels of ozone.-Science 160: 1122, 1968.
Karnaukhov, V.N.: On the nature and function of yellow ageing pigment lipofuscin.-Exp. Cell Res. 80: 479–483, 1973.
Karnaukhov, V.N.: Lyuminestsentnyï Spektral'nyï Analiz Kletki. [Luminescent Spectral Analysis of the Cell.]-Nauka, Moskva 1978. [In Russ.]
Karnaukhov, V.N.: Biologicheskie Funktsii Karotinoidov. [Biological Functions of Carotenoids.]-Nauka, Moskva 1988. [In Russ.]
Karnaukhov, V.N.: Carotenoids.-Comp. Biochem. Physiol. 95: 1–25, 1990.
Melnikova, E.V., Roshchina, V.V., Karnaukhov, V.N.: [Microspectrofluorimetry of pollen.]-Biofizika 42: 226–233, 1997. [In Russ.]
Merzlyak, M.N.: Liposoluble fluorescent "aging pigments" in plants.-In: Nagy, I. (ed.): Lipofuscin-1987: State of the Art. Pp. 451–452. Akadémiai Kiadó, Budapest; Elsevier, Amsterdam 1988.
Mumford, R.A., Lipke, H., Laufer, D.A., Feder, W.A.: Ozone-induced changes in corn pollen.-Environ. Sci. Technol. 6: 427–430, 1972.
Nakada, M., Fukui, S., Kanno, S.: Effects of exposure to various injurious gases on germination of lily pollen.-Environ Pollution 11: 181–187, 1976.
Palmer, D.N., Barns, G., Husbands, D.R., Jolly, R.D.: Ceroid lipofuscinnosis in sheep.-J. biol. Chem. 261: 1766–1777, 1986.
Roshchina, V.V., Melnikova, E.V.: Microspectrofluorometry: A new technique to study pollen allelopathy.-Allelopathy J. 3: 51–58, 1996.
Roshchina, V.V., Melnikova, E.V., Kovaleva, L.V.: [Autofluorescence at the interaction pollen-pistil of Hippeastrum hybridum.]-Dokl. ross. Akad. Nauk 349: 118–120, 1996. [In Russ.]
Roshchina, V.V., Melnikova, E.V., Kovaleva, L.V.: [The changes in the fluorescence during the development of male gametophyte.]-Fiziol. Rast. 47: 45–53, 1997. [In Russ.]
Roshchina, V.V., Melnikova, E.V., Mit'kovskaya, L.I., Karnaukhov, V.N.: Microspectrofluorimetry for the study of intact plant secretory cells.-Zh. obshcheï Biol. 59: 531–554, 1998.
Shimasaki, N., Maeba, R., Tachibana, R., Ueta, N.: Lipid peroxidation and ceroid accumulation in macrophages cultured with oxidized low density lipoprotein.-Gerontology 41(Suppl. 2): 39–49, 1995.
Yin, D.: Studies on age pigments evolving into a new theory of biological ageing.-Gerontology 41(Suppl. 2): 159–171, 1995.
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Roshchina, V., Karnaukhov, V. Changes in Pollen Autofluorescence Induced by Ozone. Biologia Plantarum 42, 273–278 (1999). https://doi.org/10.1023/A:1002120904588
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DOI: https://doi.org/10.1023/A:1002120904588