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Effect of Calcium and Zinc on Subcellular Distribution, Activity and Thermosensitivity of Superoxide Dismutase in Mnium Affine

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Biologia Plantarum

Abstract

In the leafstem moss Mnium affine two superoxide dismutase (SOD) isoforms were found in chloroplasts and two in mitochondria. Four other isozymes were probably cytosolic and two of them had high activity and thermostability and were very sensitive to H2O2. On the other hand, one of the mitochondrial isoenzymes was very sensitive to high temperature. The activity and thermosensitivity of SOD was considerably dependent on calcium and zinc ions. The effect was different for the individual isoforms and related to their subcellular distribution. Calcium ions predominantly activated and stabilized one cytosolic and the mitochondrial SODs, while zinc ions influenced one chloroplastic and two cytosolic SODs.

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References

  • Asada, K., Kanematsu, S., Okaka, S., Hayakawa, T.: Phylogenetic distribution of three types of superoxide dismutase in organisms and in organelles.-In: Hill, J.V. (ed.): Chemical and Biochemical Aspects of Superoxide and Superoxide Dismutase. Pp. 136–153. Elsevier/North Holland Biomedical Press, Amsterdam 1980.

    Google Scholar 

  • Bakardjieva, N., Christova, N., Christov, K.: Effect of calcium and zinc ions on the thermosensitivity of peroxidase, superoxide dismutase and catalase in Scenedesmus acutus cells.-Comp. rend. Acad. bulg. Sci. 47(12): 83–86, 1994.

    CAS  Google Scholar 

  • Beauchamp, C. H., Fridovich, I.: Superoxide dismutase: improved assay and an assay applicable to acrylamide gels.-Anal. Biochem. 44: 276–287, 1971.

    Article  PubMed  CAS  Google Scholar 

  • Boutry, M., Nagy, F., Poulsen, C., Aoyagi, K., Chua, C.: Targeting of bacterial chloramphenicol acetytransferase to mitochondria in transgenetic plants.-Nature 328: 340–342, 1987.

    Article  PubMed  CAS  Google Scholar 

  • Bowler, C., Alliote, T., De Loose, M., van Montagu, M., Inze, D.: The induction of manganese superoxide dismutase in response to stress in Nicotiana plumbaginifolia.-EMBO J. 8: 31–38, 1989.

    PubMed  CAS  Google Scholar 

  • Bowler, C., van Montagu, M., Inze, D.: Superoxide dismutase and stress tolerance.-Annu. Rev. Plant Physiol. Plant mol. Biol. 43: 83–116, 1992.

    Article  CAS  Google Scholar 

  • Bradford, M.M.: A rapid and sensitive method for the quantification of microgram quantities of proteins utilizing the principle of protein-dye-binding.-Anal. Biochem. 72: 248–254, 1976.

    Article  PubMed  CAS  Google Scholar 

  • Christov, K., Bakardjieva, N.: Peroxidase, catalase and superoxide dismutase in long-term desiccated moss Mnium affine and their role in the process of rehydration.-Plant Peroxidase Newslett. N11: 35–44, 1998a.

    Google Scholar 

  • Christov, K., Bakardjieva, N.: Subcellular distribution of superoxide dismutase isoforms in Medicago rigidula leaves and effect of calcium and zinc ions.-Compt. rend. Acad. bulg. Sci. 51, in press, 1998b.

  • Dhindsa, R., Matowe, W.: Drought tolerance in two mosses: correlated with enzymatic defence against lipid peroxidation.-J. exp. Bot. 32: 79–93, 1981.

    CAS  Google Scholar 

  • Hayakawa, T., Kanematsu, S., Asada, K.: Occurrence of Cu,Zn-superoxide dismutase in the intrathylakoid space of spinach chloroplasts.-Plant Cell Physiol. 25: 883–889, 1984.

    CAS  Google Scholar 

  • Herouart, D., Montagu, M. V., Inze, D.: Developmental and environmental regulation of the Nicotiana plumbaginifolia cytosolic Cu/Zn-superoxide dismutase promoter in transgenic tobacco.-Plant Physiol. 104: 873–880, 1994.

    Article  PubMed  CAS  Google Scholar 

  • Kanematsu, S., Asada, K.: Cu,Zn-superoxide dismutases from the fern Equisetum arvense and the green alga Spirogyra sp.: occurrence of chloroplast and cytosol types of enzyme.-Plant Cell Physiol. 30: 717–727, 1989a.

    CAS  Google Scholar 

  • Kanematsu, S., Asada, K.: Cu,Zn-superoxide dismutases in rice: occurrence of an active, monomeric enzyme and two types of isozymes in leaf and non-photosynthetic tissue.-Plant Cell Physiol. 30: 381–391, 1989b.

    CAS  Google Scholar 

  • Kwiatowski, J., Kaninga, Z.: Isolation and characterization of cytosolic and chloroplast isoenzyme of Cu,Zn-superoxide dismutase from tomato leaves and their relationship to other Cu,Zn-superoxide dismutases.-Biochim. biophys. Acta 874: 99–115, 1986.

    CAS  Google Scholar 

  • Ogawa K., Kanematsu, S., Asada, K.: Generation of superoxide anion and localization of CuZn-superoxide dismutase in vascular tissue of spinach hypocotyls: their association with lignification.-Plant Cell Physiol. 38: 1118–1126, 1997.

    PubMed  CAS  Google Scholar 

  • White, J.A., Scandalios, J.G.: Deletion analysis of the maize mitochondrial superoxide dismutase transit peptide.-Proc. nat. Acad. Sci. USA 86: 3534–3538, 1989.

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Institute of Plant Physiology, Bulgarian Academy of Sciences, Acad, G. Bonchev Str. 21, BG-1113, Sofia, Bulgaria

    K. Christov & N.T. Bakardjieva

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  1. K. Christov
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  2. N.T. Bakardjieva
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Christov, K., Bakardjieva, N. Effect of Calcium and Zinc on Subcellular Distribution, Activity and Thermosensitivity of Superoxide Dismutase in Mnium Affine. Biologia Plantarum 42, 57–63 (1999). https://doi.org/10.1023/A:1002111224635

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