Abstract
The present study pertains to the effect of different concentration of NaCl on the contents of proteins, free amino acids, proline and glycine betaine in leaves, stems and roots of Ipomoea pes-caprae. The protein content of the tissues increased in response to salinity upto 200 mM NaCl; the free amino acids content showed a reversal trend. The proline and glycine betaine contents increased with increasing salinity upto 500 mM NaCl. The accumulation of proline and glycine betaine might play a role in the alleviation of salt stress.
This is a preview of subscription content, access via your institution.
References
Abbas, M.A., Younis, M.E., Shukry, W.M.: Plant growth, metabolism and adaptation in relation to stress conditions. XIV. Effect of salinity on the internal solute concentrations in Phaseolus vulgaris.-J. Plant Physiol. 138: 722-727, 1991.
Bates, L.S., Waldren, R.P., Teare, I.D.: Rapid determination of the free proline in water stress.-Plant Soil 38: 205-208, 1973.
Buhl, M.D., Stewart, C.R.: Effects of NaCl on proline synthesis and utilization in excised barley leaves.-Plant Physiol. 72: 664-667, 1983.
Das, N., Misra, M., Misra, A.N.: Sodium chlorides salt stress induced metabolism changes in callus cultures of pearl millet (Pennisetum americanum L.): free solute accumulation.-J. Plant Physiol. 137: 244-246, 1990.
Delauney, A.J., Verma, D.P.S.: Proline biosynthesis and osmoregulation in plants.-Plant J. 4: 215-223, 1993.
Grieve, C.M., Grattan, S.R.: Rapid assay for determination of water soluble quaternary ammonium compounds.-Plant Soil 70: 303-307, 1983.
Hanson, A.D., Nelson, C.E.: Betaine accumulation and [14C] formate metabolism in water stressed barley leaves.-Plant Physiol. 62: 305-312, 1978.
Lowry, O.H., Rosenbrough, N.J., Farr, A.L., Randall, R.J.: Protein measurement with the Folin phenol reagent.-J. Biol. Chem. 193: 265-275, 1951.
McDonnel, E., Wyn Jones, R.G.: Glycine betaine biosynthesis and accumulation in unstressed and stressed wheat.-J. Exp. Bot. 201: 421-430, 1988.
Moore, S., Stein, W.H.: Photometric method for use in the chromatography of aminoacids.-J. Biol. Chem. 176: 367-388, 1948.
Pérez-Alfocea, F., Santa-Cruz, A., Guerrier, G., Bolarín, M.C.: NaCl stress induced organic solute changes on leaves and calli of Lycopersicon esculentum and L. pennellii and their interspecific hybrid.-J. Plant Physiol. 143: 106-111, 1994.
Rao, G.C., Rao, G.R.: 14CO2 incorporation into leaves of pigeon-pea and sesame under salt stress.-J. nucl. Agr. Biol. 10: 123-126, 1981.
Rhodes, D., Hanson, A.D.: Quaternary ammonium and terciary sulfonium compounds in higher plants.-Annu. Rev. Plant Physiol. Plant mol. Biol. 44: 357-384 1993.
Shaddad, M.A., Radi, A.F., Abdel-Rahman, A.M., Azooz, M.M.: Response of seeds of Lupinus termis and Vicia faba to the interactive effect of salinity and ascorbic acid or pyridoxine.-Plant Soil 122: 177-183, 1990.
Storey, R., Wyn Jones, R.G.: Quaternary ammonium compounds in plants in relation to salt resistance.-Phytochemistry 16: 447-453, 1977.
Wyn Jones, R.G., Storey, R.: Betaines.-In: Paleg, L.G., Aspinall, D. (ed.): The physiology and Biochemistry of Drought Resistance in Plants. Pp. 171-204. Academic Press, Sydney-New York-Toronto-London-San Francisco 1981.
Zidan, M.A.: Proline synthesis and degradation in salinized Chlorella pyrenoidosa.-Indian J. Plant Physiol. 38: 118-120, 1995.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Venkatesan, A., Chellappan, K. Accumulation of proline and glycine betaine in Ipomoea pes-caprae induced by NaCl. Biologia Plantarum 41, 271–276 (1998). https://doi.org/10.1023/A:1001839302627
Issue Date:
DOI: https://doi.org/10.1023/A:1001839302627