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Repression of Genetic Activity in Root Meristem Cells by Peptidic Factor Derived from Male Sex Organs of Chara

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Biologia Plantarum

Abstract

Present autoradiographic investigations using 3H-uridine and 3H-leucine incorporation indicate that preincubation of primary roots of Melandrium noctiflorum with antheridial chromatin condensation factor (ACCF), a low molecular mass peptidic factor (4.5 kDa) obtained from maturing male sex organs of Chara tomentosa, leads to a marked reduction of transcription and translation in G1, G2 and endopolyploid cells (8C DNA level). Moreover, silver staining of cells reveals considerable decrease in number and sizes of nucleoli. The similarities that exist between the "innate" processes within developing antheridial filaments of Chara and the genetic repression induced by antheridial peptidic extracts in root cells of M. noctiflorum seems to provide further positive evidence for the presence of a highly specific factor that contributes to nuclear re-patterning of differentiating germ line cells in male sex organs of Chara.

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Authors and Affiliations

  1. Department of Cytophysiology, University of Łodź, ul., 90 231, Łodź, Poland

    J. Maszewski & A. Kaźmierczak

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  1. J. Maszewski
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  2. A. Kaźmierczak
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Maszewski, J., Kaźmierczak, A. Repression of Genetic Activity in Root Meristem Cells by Peptidic Factor Derived from Male Sex Organs of Chara. Biologia Plantarum 41, 357–368 (1998). https://doi.org/10.1023/A:1001834007595

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