Abstract
The excised shoot tips of Beta vulgaris L. cvs. Ras poly, Gala and Lola were inoculated aseptically on Murashige and Skoog's (MS) medium with 0.25 mg dm-3 α-naphthaleneacetic acid (NAA) and 1.00 mg dm-3 6-benzylaminopurine (BAP). They produced multiple shoots at relatively high frequency. For shoot multiplication, a concentration of 1.00 mg dm-3 NAA and 0.25 mg dm-3 BA in the culture medium was found to be optimum for all the cultivars. MS medium supplemented with 3.0 mg dm-3 3-indolebutyric acid (IBA) and 0.02 mg dm-3 N-isopentenylaminopurine (2ip) was found most effective for rooting of micropropagated shoots. In vitro produced plantlets were successfully transferred to the soil. The percentage of survival was more than 95 % and micropropagated plants showed no morphological differences from those grown naturally.
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Rady, M. In Vitro Multiplication of Beta Vulgaris L. throughout Excised Shoot Tips. Biologia Plantarum 40, 515–522 (1997). https://doi.org/10.1023/A:1001792818216
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DOI: https://doi.org/10.1023/A:1001792818216