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Biosynthesis of glycoproteins in Candida albicans: Biochemical characterization of dolichol phosphate glucose synthase

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Abstract

A mixed membrane fraction isolated from C. albicans yeast cells catalyzed the transfer of glucose from UDP-Glc into three classes of endogenous acceptors: glucolipid, glycoprotein and lipid-linked oligosaccharides. About 80μ of the total radioactivity transferred into these products corresponded to the glucolipid which was identified as dolichol phosphate glucose by several criteria. The remainder was detected in about equal proportions in the other two fractions. Conditions that stimulated or inhibited glucolipid synthesis did not affect the extent of glycoprotein labeling. The synthesis of dolichol phosphate glucose exhibited a Kmof 104 μM UDP-Glc and was stimulated by Mg2+but not by Mn2+or Ca2+. The latter cations were, however, better stimulators of glycoprotein labeling than Mg2+. Most nucleotides strongly inhibited the synthesis of dolichol phosphate glucose, UMP being a competitive inhibitor with a Kiof 100 μM. The dolichol phosphate glucose synthase reaction was reversed about 57μ by 0.62 mM UDP but not by UMP.

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Authors and Affiliations

  1. Instituto de Investigación en Biología Experimental, Facultad de Química, Universidad de Guanajuato, Apartado Postal 187, Guanajuato, Gto, 36000, México

    Jesús Rodríguez-Bonilla, Lorena Vargas-Rodríguez, Carlos Calvo-Méndez, Arturo Flores-Carreón, Everardo López-Romero & Everardo López-Romero

  2. Departamento de Genética y Biología Molecular, CINVESTAV del IPN, Apartado Postal 14-740, México, DF 07000, México

    Carlos Calvo-Méndez, Arturo Flores-Carreón & Everardo López-Romero

Authors
  1. Jesús Rodríguez-Bonilla
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  2. Lorena Vargas-Rodríguez
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  3. Carlos Calvo-Méndez
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  4. Arturo Flores-Carreón
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  5. Everardo López-Romero
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Correspondence to Everardo López-Romero.

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Rodríguez-Bonilla, J., Vargas-Rodríguez, L., Calvo-Méndez, C. et al. Biosynthesis of glycoproteins in Candida albicans: Biochemical characterization of dolichol phosphate glucose synthase. Antonie Van Leeuwenhoek 73, 373–380 (1998). https://doi.org/10.1023/A:1001714623355

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  • DOI: https://doi.org/10.1023/A:1001714623355

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