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A simple procedure for the detection of plant extracellular proteolytic enzymes

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Biologia Plantarum

Abstract

A simple procedure for the detection of extracellular plant proteolytic enzymes using insoluble dye stained gelatin substrates incorporated into an appropriate culture medium is described. Extracellular proteinases produced by the tested plant cells (callus culture and cell suspension) hydrolyzed the substrates and dyed peptide fragments were released. Dyed zones around and under the proteinase-producing callus cultures were formed on the agar medium. Similarly, coloration of the culture media using proteinase-producing cell suspensions was observed.

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Authors and Affiliations

  1. Garden of Medical Plants, Faculty of Pharmacy, Comenius University, Odbojárov 10, 832 32, Bratislava, Slovak Republic

    J. Stano, P. Kovács, I. Šafařík, D. Kákoniová & M. Šafaříková

  2. Department of Cell and Molecular Biology of Drugs, Faculty of Pharmacy, Comenius University, Odbojárov 10, 832 32, Bratislava, Slovak Republic

    P. Kovács

  3. Laboratory of Biochemistry and Biotechnology, Institute of Landscape Ecology, Academy of Sciences of the Czech Republic, Na sádkách 7, 370 05, České Budějovice, Czech Republic

    I. Šafařík & M. Šafaříková

  4. Institute of Chemistry, Slovak Academy of Sciences, Dúbravská cesta 9, 842 38, Bratislava, Slovak Republic

    D. Kákoniová

Authors
  1. J. Stano
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  2. P. Kovács
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  3. I. Šafařík
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  4. D. Kákoniová
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  5. M. Šafaříková
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Correspondence to J. Stano.

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Stano, J., Kovács, P., Šafařík, I. et al. A simple procedure for the detection of plant extracellular proteolytic enzymes. Biologia Plantarum 40, 475–477 (1997). https://doi.org/10.1023/A:1001194704203

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