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Responses of alfalfa to potassium, calcium, and nitrogen under stress induced by sodium chloride

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Biologia Plantarum

Abstract

The physiological responses of alfalfa (Medicago sativa L. cv. Gilboa) to salinity (100 mM NaCl) and some inorganic nutrients (K+, Ca2+ and N as NO3-) were investigated. Salinity caused a substantial reduction in biomass, carbon assimilation rate, stomatal conductance, water use efficiency, leaf area, relative growth rate, NO3- content and nitrate reductase activity, whereas, transpiration rate was slightly affected. Inclusion of K+, Ca2+ and N as NO3- in plant nutrient medium in combination or alone brought about a marked stimulation in control plants and moderated the salinity caused reductions in NaCl treated plants. In addition, plants also exhibited differences in these parameters at two growth stages.

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References

  • Aslam, M., Huffaker, R.C., Rains, D.W.: Early effects of salinity on nitrate assimilation in barley seedlings.-Plant Physiol. 76: 321–325, 1984.

    PubMed  CAS  Google Scholar 

  • Ball, M.C., Farquhar, G.D.: Photosynthetic and stomatal responses of two mangrove species. Aegiceros corniculatum and Avicennia marina to long term salinity and humidity conditions.-Plant Physiol. 74: 1–6, 1984.

    PubMed  Google Scholar 

  • Cataldo, D.S., Haroon, M., Schrader, L.E., Youngs, V.L.: Rapid colorimetric determination of nitrate in plant tissues by nitration of salicylic acid.-Commun. Soil Sci. Plant Anal. 6: 71–80, 1975.

    CAS  Google Scholar 

  • Downton, W.J.S.: Photosynthesis in salt stressed grapevines.-Plant Physiol. 4: 183–192, 1977.

    CAS  Google Scholar 

  • Downton, W.J.S., Grant, W.J.R., Robinson, S.P.: Photosynthetic and stomata responses of spinach leaves to salt stress.-Plant Physiol. 77: 85–88, 1985.

    Google Scholar 

  • Gale, J., Kohl, H.C., Hagan, R.M.: Changes in water halance and photosynthesis of onion, bean and cotton plants under saline conditions.-Physiol. Plant 20: 468–520, 1967.

    Article  Google Scholar 

  • Gouia, H., Ghorbal, M.H., Touraine, B.: Effect of NaCl on flows of N and mineral ions and on NO3 reduction rate within whole plants of salt sensitive bean and salt tolerant cotton.-Plant Physiol. 105: 1409–1418, 1994.

    PubMed  CAS  Google Scholar 

  • Greenway, H., Munns, R.: Mechanisms of salt tolerance in nonhalophytes.-Annu. Rev. Plant Physiol. 31: 149–190, 1980.

    Article  CAS  Google Scholar 

  • Hawkins, H.J., Lewis, O.A.M.: Combination effect of NaCl salinity, nitrogen forms and calcium concentration on the growth, ionic content and gaseous exchange properties of Triticum aestivum L. ev. Gamtoos.-New Phytol. 124: 161–170, 1993.

    Article  CAS  Google Scholar 

  • Hunt, R.: Plant Growth Analysis.-Edward Arnold Ltd., London 1978.

    Google Scholar 

  • Kaiser, W.M., Webb, H., Sauer, M.: Photosynthetic capacity, osmotic responses and solute content of leaves and chloroplasts from Spinacea oleracea under salt stress.-Z. Pflanzenphysiol. 135: 15–27, 1983.

    Google Scholar 

  • Lips, H.S., Leidi, E.O., Soarcs, M.I.M., Silberbush, M., Lewis, O.A.M.: Physiological aspects of ammonium and nitrate fertilization.-J. Plant Nutr. 13: 1271–1289, 1990.

    CAS  Google Scholar 

  • Longstreth, D.J., Strain, B.R.: Effect of salinity and illumination on photosynthesis and water balance of Spartina alternifolia.-Oecologia 31: 191–199, 1977.

    Article  Google Scholar 

  • Martinez, V., Cerda, A.: Nitrate reductase activity in tomato and cucumber leaves as influenced by NaCl and N source.-J. Plant Nutr. 12: 1335–1350, 1989.

    CAS  Google Scholar 

  • Misra, N., Dwivedi, U.N.: Nitrogen assimilation in germinating Phaseolus aureus seeds under saline stress.-J. Plant Physiol. 135: 719–724, 1990.

    CAS  Google Scholar 

  • Neumann, P.M., Van Volkenburgh, E., Cleland, R.E.: Salinity stress inhibits the bean leaf expansion by reducing turgor, not wall extensibility.-Plant Physiol. 88: 233–237, 1988.

    PubMed  CAS  Google Scholar 

  • Ourry, A., Meste, S., Bocaud, J.: Effect of osmotic stress (NaCl and polyethylenglycol) on nitrate uptake, translocation, storage and reduction in rye grass (Lolium perenne L.).-New Phytol. 120: 275–280, 1992.

    Article  CAS  Google Scholar 

  • Plaut, Z., Grieve, C.M., Federman, E.: Salinity effects on photosynthesis in isolated mesophyll cells of cowpea leaves.-Plant Physiol. 91: 493–499, 1989.

    PubMed  CAS  Google Scholar 

  • Rao, R.K., Gnanam, A.: Inhibition of nitrate and nitrite reductase activities by salinity stress in Sorghum vulgare.-Phytochemistry 29: 1047–1049, 1990.

    Article  CAS  Google Scholar 

  • Silberbush, M., Lips, S.H.: Nitrogen concentration, ammonium/nitrate ratio and NaCl interaction in vegetation and reproductive growth of peanuts.-Physiol. Plant. 74: 493–498, 1988.

    Article  CAS  Google Scholar 

  • Seeman, J.R., Sharkey, T.D.: Salinity and nitrogen effects on photosynthesis, ribulose-1,5-bisphosphate carboxylase and metabolite pool sizes in Phaseolus vulgaris L.-Plant Physiol. 82: 555–560, 1986.

    Article  Google Scholar 

  • Seeman, J.R., Critchley, C.: Effects of salt stress on the growth, ion content, stomatal behaviour and photosynthetic capacity of salt-sensitive species, Phaseolus vulgaris L.-Planta 164: 151–162, 1985.

    Article  Google Scholar 

  • Soares, M.I.M., Lips, S.H., Cresswell, C.F.: Endogenous nitrate loss as an assay for nitrate reduction in vivo.-Physiol. Plant. 64: 487–491, 1985.

    Article  CAS  Google Scholar 

  • Termaat, A., Munns, R.: Use of concentrated macronutrient solutions to separate osmotic from NaCl-specific effects on plant growth.-Aust. J. Plant Physiol. 13: 509–552, 1986.

    Article  CAS  Google Scholar 

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Khan, M., Silberbush, M. & Lips, S. Responses of alfalfa to potassium, calcium, and nitrogen under stress induced by sodium chloride. Biologia Plantarum 40, 251–259 (1997). https://doi.org/10.1023/A:1001072704686

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