Abstract
Cell suspension culture Amsonia tabernaemontana Walt. were permeabilized by Tween 80 and immobilized by glutaraldehyde. The highest α-galactosidase activity was at pH 5.3 and temperature 70 °C. The hydrolysis of substrate was linear for 3 h reaching 70 - 75 % conversion. The cells characterized by high enzyme activity and stability in long-term storage showed convenient physico-mechanical properties (physical protection from shear forces and easy separation of product from biocatalysts).
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Poór, J. Activity of α-galactosidase in immobilized cells of Amsonia tabernaemontana Walt. Biologia Plantarum 40, 161–167 (1997). https://doi.org/10.1023/A:1001060100143
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DOI: https://doi.org/10.1023/A:1001060100143