Abstract
Culture conditions for a fine dispersion of plated cells of Oryza sativa L. cv. IR 20, have been worked out. These plated cells developed microcalli containing large number of somatic embryos and subsequently plantlets. By using single cells and clusters of 2 - 4 cells, an efficient DNA-delivery by microprojectile bombardment into cells and its transient expression were assessed by employing a plasmid construct containing β-glucuronidase gene.
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Sathishkumar, R., Agrawal, S. & Manoharan, K. Particle mediated DNA delivery and transient expression of GUS gene in plated cells of rice. Biologia Plantarum 40, 305–309 (1997). https://doi.org/10.1023/A:1001044008321
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DOI: https://doi.org/10.1023/A:1001044008321