Abstract
The effects of ferulic acid on L-malate oxidation in mitochondria isolated from soybean (Glycine max L.) seedlings were investigated. Oxygen uptake and the products of L-malate oxidation were measured under two conditions: pH 6.8 and 7.8. At acidic pH, the activity of the NAD+-linked malic enzyme (L-malate:NAD+oxidoreductase [decarboxylating] EC 1.1.1.39) was favoured, whereas at alkaline pH a predominance of the L-malate dehydrogenase activity (L-malate:NAD+oxidoreductase EC 1.1.1.37) was apparent. Ferulic acid inhibited basal and coupled respiration during L-malate oxidation either at acidic or alkaline pH, reducing also the amounts of pyruvate or oxaloacetate produced. The results suggest that the site of ferulic acid action is situated at some step that precedes the respiratory chain. An interference with the L-malate entry into the mitochondria could be an explanation for the effects of ferulic acid, but the possibility of a direct inhibition of both enzymes involved in L-malate oxidation cannot be ruled out.
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References
Bonner, J.: The role of toxic substances in the interaction of higher plants.-Bot. Rev. 16: 51-65, 1950.
Day, D.A., Hanson, J.B.: On methods for the isolation of mitochondria from etiolated corn shoots.-Plant Sci. Lett. 11: 99-1044, 1977.
Demos, E.K., Woolwine, M., Wilson, R.H., McMillan, C.: The effects of ten phenolic compounds on hypocotyl growth and mitochondrial metabolism of mung bean.-Amer. J. Bot. 62: 97-102, 1975.
Einhellig, F.A.: Mechanism and mode of action of allelochemicals.-In: Putnam, A.R., Tang, C.S. (ed.): The Science of Allelopathy. Pp. 171-188. John Wiley & Sons, New York 1986.
Einhellig, F.A., Muth, M.S.: Ferulic acid interference with water metabolism in grain sorghum. Proc. S. Dak. Acad. Sci. 5: 276, 1980.
Glass, A.D.M.: Influence of phenolic acids on ion uptake. I. Inhibition of phosphate uptake.-Plant Physiol. 51: 1037-1041, 1973.
Harper, J.R., Balke, N.E.: Characterization of the inhibition of K+ absorption in oat roots by salicylic acid.-Plant Physiol. 68: 1340-1353, 1981.
Kefeli, V.I., Kadyrov, C.S.: Natural growth inhibitors, their chemical and physiological properties.-Annu. Rev. Plant Physiol. 22: 185-196, 1971.
Lowry, O.H., Rosebrough, N.J., Farr, A.C., Randal R.J.: Protein measurements with the Folin phenol reagent.-J. biol. Chem. 193: 265-275, 1951.
MacRae, A.R.: Effect of pH on the oxidation of malate by isolated cauliflower bud mitochondria.-Phytochemistry 10: 1453-1458, 1971.
Moreland, D.E., Novitsky, W.O.: Effects of phenolic acids, and flavonoides on isolated chloroplasts and mitochondria.-In: Waller, G.R. (ed.): Symposium on Allelochemicals: Role in Agriculture, Forestry, and Ecology. Pp. 247-261. American Chemical Society, Washington 1986.
Muller, C.H.: The role of chemical inhibition (allelopathy) in vegetal composition.-Bull. Torrey bot. Club 93: 332-351, 1966.
Palmer, J.M.: The organization of electron transport in plant mitochondria.-Annu. Rev. Plant Physiol. 27: 133-157, 1976.
Patterson, D.T.: Effects of allelopathic chemicals on growth and physiological responses of soybeans (Glycine max).-Weed Sci. 29: 53-59, 1981.
Ray, S.D., Guruprasad, K.N., Laloraya, M.M.: Antagonistic action of phenolic compounds on abscisic acid-induced inhibition of hypocotyl growth.-J. exp. Bot. 31: 1651, 1980.
Sathiyamoorthy, P.: Identification of vanillic and p-coumaric acid as endogenous inhibitors of soybean seeds and their inhibitory effect on germination.-Plant Physiol. 136: 120-121, 1990.
Van Sumere, C.F., Cottenie, J., De Greef, J., Kint, J.: Biochemical studies in relation to the possible germination regulatory role of naturally occuring coumarin and phenolics.-Rec. Adv. Phytochem. 4: 165-221, 1972.
Wang, T.S.C., Yeh, S.Y., Cheng, S.Y., Yang, T.K.: Behavior of soil phenolic acids.-In: U.S. Nat. Comm. IBP (ed.): Biochemical Interaction Among Plants. Pp. 113-120. National Academy of Science, Washington 1971.
Wedding, R.T., Black, M.K., Pap, D.: Malate dehydrogenase and NAD malic enzyme in the oxidation of malate by sweet potato mitochondria.-Plant Physiol. 58: 740-743, 1976.
Whittaker, R.H., Feeny, P.P.: Allelochemicals: Chemical interactions between species.-Science 171: 757-767, 1971.
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Sert, M., Ferraresi, M., Bernadelli, Y. et al. Effects of ferulic acid on L-malate oxidation in isolated soybean mitochondria. Biologia Plantarum 40, 345–350 (1997). https://doi.org/10.1023/A:1001005711955
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DOI: https://doi.org/10.1023/A:1001005711955