Antonie van Leeuwenhoek

, Volume 73, Issue 1, pp 127–141 | Cite as

Application of denaturing gradient gel electrophoresis (DGGE) and temperature gradient gel electrophoresis (TGGE) in microbial ecology

  • Gerard Muyzer
  • Kornelia Smalla
Article

Abstract

Here, the state of the art of the application of denaturing gradient gel electrophoresis (DGGE) and temperature gradient gel electrophoresis (TGGE) in microbial ecology will be presented. Furthermore, the potentials and limitations of these techniques will be discussed, and it will be indicated why their use in ecological studies has become so important.

DGGE genetic fingerprinting microbial ecology molecular microbial ecology PCR rRNA TGGE 

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© Kluwer Academic Publishers 1998

Authors and Affiliations

  • Gerard Muyzer
    • 1
  • Kornelia Smalla
    • 2
  1. 1.Max-Planck-Institute for Marine MicrobiologyBremenGermany
  2. 2.Biologische Bundesanstalt für Land- und ForstwirtschaftBraunschweigGermany

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