Abstract
Forty Aspergillus japonicus and A. aculeatus strains, most of them wild-type isolates, were examined using various molecular and phenotypic techniques. The rDNAs proved to be invariable (even strains of the species A. aculeatus exhibited the same restriction profile), while the strains could be classified into seven different mtDNA RFLP groups. Hybridisation data suggest that six of these mtDNA types have certain common restriction sites, while mtDNA type 7, which was exhibited by some A. aculeatus strains, probably has quite different mtDNA organisation and their size was smallest among the strains studied. The RAPD technique and isoenzyme analysis revealed some variabilities within these RFLP groups and strain specific features could also be recognised. Carbon source assimilation spectra were found to be very distinctive for strains of A. japonicus, A. aculeatus and A. niger, providing a useful tool for pre-characterising new wild-type isolates of black Aspergilli. Only a limited correlation was observed between the dendrograms based on genotypic and phenotypic characters.
Similar content being viewed by others
References
Al-Musallam, A., (1980). Revision of the black Aspergillus species. Ph.D. Thesis, State University Utrecht, The Netherlands.
Burnie, J.P., A. Coke & R.C. Matthews, (1992). Restriction endonuclease analysis of Aspergillus fumigatus DNA. J Clin Pathol 45: 324–327.
Borsuk, P.A., M.N. Nagiec, P.P. Stepien & E. Bartnik, (1982). Organization of the ribosomal RNA gene cluster in Aspergillus nidulans. Gene 17: 147–152.
Davis, B.J., (1964). Disc electrophoresis-II. Method and application to human serum proteins. Ann. New York Acad Sci 121: 404–423.
Gams, W., M. Christensen, A.H.S. Onions, J.I. Pitt & R.A. Samson, (1985). Infrageneric taxa of Aspergillus. In: R.A. Samson & J.I. Pitt (Eds.), Advances in Penicillium and Aspergillus Systematics, (pp. 55–62). Plenum Press, New York.
Jadeyel, D.M., (1986). Variation in the organization and structure of the mitochondrial DNA of species of Aspergillus. Ph.D. Thesis, University of Birmingham, Birmingham, UK.
Kálmán, É.T., J. Varga & F. Kevei, (1991). Characterization of interspecific hybrids within the Aspergillus nidulans species group by isoenzyme analysis. Can J Microbiol 37: 391–396.
Kevei, F., Zs. Hamari, J. Varga, Z. Kozakiewicz & J.H. Croft, (1996). Molecular polymorphism and phenotypic variation in Aspergillus carbonarius. Ant van Leeuwenhoek 70: 59–66.
Kevei, F., B. Tóth, A. Coenen, Z. Hamari, J. Varga & J.H. Croft, (1997). Recombination of mitochondrial DNAs following transmission of mitochondria among incompatible strains of black Aspergilli. Mol Gen Genet 254: 379–388.
Kozakiewicz, Z., (1989). Aspergillus species on stored products. Mycol Papers 161: 1–188.
Kusters-van Someren, M.A., R.A. Samson & J. Visser, (1990). Variation in pectinolytic enzyme of black Aspergilli: biochemical and genetical approach. In: R.A. Samson & J.I. Pitt (Eds.), Modern Concepts in Penicillium and Aspergillus classification. NATO ASI Series vol. 165 (pp. 321–334) Plenum Press, New York.
Kusters-van Someren, M.A., R.A. Samson & J. Visser, (1991). The use of RFLP analysis in classification of the black Aspergilli: reinterpretation of the Aspergillus niger aggregate. Curr Genet. 19: 21–26.
Leach, J., D.B. Finkelstein & J.A. Rambosek, (1986). Rapid miniprep of DNA from filamentous fungi. Fungal Genet Newslett 33: 32–33.
Lehmann, P.F., B.J. Kemker, C.B. Hsiao & S. Dev, (1989). Isoenzyme biotypes of Candida species. J Clin Microbiol 27: 2514–2521.
Lehmann, P.F., U. Khazan, L.C. Wu, B.I. Wickes & K.J. Kwon-Chung, (1992). Karyotype and isozyme profiles do not correlate in Kluyveromyces marxianus var. marxianus. Mycol Res 96: 637–642.
Manczinger, L. & G. Polner, (1987). Cluster analysis of carbon source utilization patterns of Trichoderma isolates. System Appl Microbiol 9: 214–217.
Megnegneau, B., F. Debets & R. Hoekstra, (1993). Genetic variability and relatedness in the complex group of black Aspergilli based on random amplification of polymorphic DNA. Curr Genet 23: 323–329.
Osman, K.E., (1987). Genetic and molecular studies of Aspergillus. Ph.D. Thesis, University of Birmingham, Birmingham, UK.
Podani, J., (1993). SYN-TAX-pc. Computer programs for multivariate data analysis in ecology and systematics (version 5.0 user's guide). Scientia Publishing, Budapest, Hungary.
Pontecorvo, G., J.A. Roper, L.M. Hemmons, K.D. MacDonald & A.W.J. Bufton, (1953). The genetics of Aspergillus nidulans. Adv Genet 5: 141–238.
Raper, K.B. & D.I. Fennell, (1965). The genus Aspergillus. Williams and Wilkins Company, Baltimore.
Rinyu, E., J. Varga & L. Ferenczy, (1995). Phenotypic and genotypic analysis of variability in Aspergillus fumigatus. J Clin Microbiol 33: 2567–2575.
Sambrook, J., E.F. Fritsch & T. Maniatis, (1989). Molecular cloning. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York.
Sneath, P.H.A. & R.R. Sokal, (1973). Numerical taxonomy. W. H. Freeman and Co., San Francisco, California.
Varga, J., F. Kevei, Cs. Fekete, A. Coenen, Z. Kozakiewicz & J.H. Croft, (1993). Restriction fragment length polymorphisms in the mitochondrial DNAs of the Aspergillus niger aggregate. Mycol Res 97: 1207–1212.
Varga, J., F. Kevei, A. Vriesema, F. Debets, Z. Kozakiewicz & J.H. Croft, (1994). Mitochondrial DNA restriction fragment length polymorphisms in field isolates of the Aspergillus niger aggregate. Can J Microbiol 40: 612–621.
Varga, J., C. Vágvölgyi, Á. Nagy, I. Pfeiffer & L. Ferenczy, (1995). Isoenzyme, restriction fragment length polymorphism, and random amplified polymorphic DNA characterization of Phaffia rhodozyma Miller et al., Int J Syst Bacteriol 45: 173–177.
Varga, J., E. Rinyu, I. Kiss, B. Botos & Kozakiewicz, (1997). Carbone source utilization and isoenzyme analysis as taxonomic aids for toxicogenic Neosartorya species and their relatives. Acta Microbiol Immunol Hung 44: 1–11.
Visser, J., M. Suykerbuyk, M.A. Kusters-van Someren, R.A. Samson & P. Schaap, (1996). Classification of black Aspergilli by RFLP analysis. In: L. Rossen, V. Rubio, M.T. Dawson & J. Frisvad (Eds.), Fungal Identification Techniques. European Commission EUR 16510EN pp. 194–201.
Williams, J.G., A.R. Kubelik, K.J. Livak, J.A. Rafalski & S.V. Tingey, (1990). DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res 18: 6531–6535.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Hamari, Z., Kevei, F., Kovács, É. et al. Molecular and phenotypic characterization of Aspergillus japonicus and Aspergillus aculeatus strains with special regard to their mitochondrial DNA polymorphisms. Antonie Van Leeuwenhoek 72, 337–347 (1997). https://doi.org/10.1023/A:1000578913759
Issue Date:
DOI: https://doi.org/10.1023/A:1000578913759