Abstract
Oleaginous micro-organisms (yeasts, moulds), in culture media having the carbon source as limited factor, degrade reserve lipids and produce new biomass, after the onset of carbon exhaustion from the medium. In this paper the process of lipid accumulation-degradation in oleaginous micro-organisms, growing on a vegatable oil was simulated. The model was integrated with 4 different methods and the parameters were optimised with the least squares method. It was found that the degradation of endocellular carbon pool is a very slow process characterised, however, by a good yield in fat-free biomass. Low values of the specific growth rates of the fat-free microbial mass, both from consumption of extra cellular and endocellular carbon pools, favourite the production of microbial lipid. The maximum of the specific rate of lipid accumulation is positively affected by the low values of the specific growth rate of the fat-free microbial mass from consumption of extra cellular carbon pool, but remained unaffected by the specific growth rate of the fat-free microbial mass from consumption of endocellular carbon pool. On the other hand, lipid production and specific rate of lipid accumulation are positively influenced by the high values of the specific rate of storage lipid formation. In conclusion, this numerical model can be used in the laboratory as pilot for planing further experimental work.
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Aggelis G (1989) Etude de la production d'acide gamma linolénique par des Phycomycetes (Mucorales). Thèse de Doctorat en Biochemie. Université de Montpellier II-USTL, France
Aggelis G, Komaitis M, Dimitroulias G, Pina M & Graille J (1991a) Possibilité de production d'acide gamma linolénique par culture de Mucor circinelloides CBS 172-27 sur quelques huiles végétales. Oléagineux 46: 208–212
Aggelis G, Balatsouras G, Komaitis M, Anagnostopoulou G, Dimitroulias G, Pina M & Graille J (1991b) Production d'acide gamma linolénique par bioconversion de l'acide linolénique de quelques huiles végétales. Revue Française des Corps Gras 38: 95–101
Aggelis G, Komaitis M, Pina M & Graille J (1993) Specificity of Mucor miehei lipase on methyl ester substrates. Grasas y Aceites 44: 331–334
Aggelis G, Mamalakis G & Komaitis M (1995a) Fatty acid specificity (typospecificity) of some microbial lipases. La Rivista Italiana delle Sostanze Grasse LXXII: 211–213
Aggelis G, Komaitis M, Papanikolaou S & Papadopoulos G (1995b) A mathematical model for the study of lipid accumulation in oleaginous microorganisms: I. Lipid accumulation during growth of Mucor circinelloides CBS 172-27 on a vegetable oil. Grasas y Aceites 46: 169–173
____ (1995c) A mathematical model for the study of lipid accumulation in oleaginous microorganisms: II. Study of cellular lipids of Mucor circinelloides during growth on a vegetable oil. Grasas y Aceites 46: 245–250
Aggelis G (1996) Two alternative pathways for substrate assimilation by Mucor circinelloides. Folia Microbiol. 41: 254–256
Aggelis G, Stathas D, Tavoularis N & Komaitis M (1996) Composition of lipids produced by some strains of Candida species. Production of single cell oil in chemostat culture. Folia Microbiol. 41: 299–302
Aggelis G, Papadiotis G & Komaitis M (1997) Microbial fatty acid specificity. Folia Microbiol. 42: in press
Botham PA & Ratledge C (1979) A biochemical explanation for lipid accumulation in Candida 107 and other oleaginous microorganisms. J. Gen. Microbiol. 114: 361–375
____ (1983) A comparison of the oleaginous yeast, Cundida curvata, grown on different carbon sources in continuous and batch culture. Lipids 18: 623–629
____ (1985) The physiological significance of citric acid in the control of metabolism in lipid accumulating yeasts. Biotechnol. Gen. Engin. Rev. 3: 349–375
Floetenmeyer MD, Glatz BA & Hammond EG (1985) Continuous culture fermentation of the whey permeate to produce micribial oil. J. Dairy Sci. 68: 633–637
Fuji Oil Co., Ltd. (1979) Method for producing cacao butter substitute. Br. Pat. 1 555 000
Gill CO, Hall MJ & Ratledge C (1979) Lipid accumulation in an oleaginous yeast (Candida 107) growing on glucose in single-stage continuous culture. Appl. Environ. Microbiol. 33: 231–239
Glatz BA, Hammond EG, Hsu KH, Baehman L, Bati N, Bednarski W, Brown D & Floetenmeyer M (1984) Production and modification of fats and oils by yeast fermentation. In: Ratledge C, Rattray JBM & Dawson PSS (Eds) Biotechnology for the Oils and Fats Industry (pp 163–176). American Oil Chemists' Society, Illinois
Holdsworth JE & Ratledge C (1988) Lipid turnover in oleaginous yeasts. J. Gen. Microbiol. 134: 339–346
Kamisaka Y, Yokochi T, Nakahara T & Suzuki O (1990) Incorporation of linoleic acid and its conversion to y-linolenic acid in fungi. Lipids 25: 54–60
Koritala S, Hesseltine CW, Pryde EH & Mounts TL (1987) Biochemical modification of fats by microorganisms: A preliminary survey. JAOCS 64: 509–513
Macrae AR (1984) Microbial lipases as catalysts for the interesterification of oils and fats. In: Ratledge C, Rattray JBM & Dawson PSS (Eds) Biotechnology for the Oils and Fats Industry (pp 189–198). American Oil Chemists' Society, Illinois
Ratledge C (1982) Microbial oils and fats: an assessment of their commercial potential. Prog. Indust. Microbiol. 16: 119–206
Ratledge C & Boulton CA (1985) Fats and oils. In: Moo-Young M (Ed.) Comprehensive Biotechnology, Vol. 3 (pp 983–1003). Pergamon Press, Oxford
Samelis J, Aggelis G & Metaxopoulos J (1993) Lipolytic and microbial changes during the natural fermentation and ripening of greek dry sausages. Meat Science 35: 371–385
Ykema A, Verbree EC, Verseveld HW & Smit H (1986) Mathematical modelling of lipid production by oleaginous yeasts in continuous cultures. Antonie van Leeuwenhoek 52: 491–506
Ykema A, Bakels R, Verwoert I, Smit H & Verseveld HW (1989) Growth yield, maintenance requirements, and lipid formation in the oleaginous yeast Apiotrichum curvatum. Biotech. Bioeng. 34: 1268–1276
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Aggelis, G., Sourdis, J. Prediction of lipid accumulation-degradation in oleaginous micro-organisms growing on vegetable oils. Antonie Van Leeuwenhoek 72, 159–165 (1997). https://doi.org/10.1023/A:1000364402110
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DOI: https://doi.org/10.1023/A:1000364402110