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A new variety of Aureobasidium pullulans characterized by exopolysaccharide structure, nutritional physiology and molecular features

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Abstract

The black yeast Aureobasidium pullulans (de Bary) Arnaud is known to synthesize the exopolysaccharide pullulan, a poly-α-1,6-maltotriose. Nine strains were found to produce additional aubasidan-like EPS, i.e. glucans with α-1,4-D-, β-1,6-D- and β-1,3-D-glycosidic bonds. These strains had previously been found to deviate in genotypic characters. Additional physiological differences were found: the optimal nitrogen source for exopolysaccharide production in liquid medium was NaNO3 for aubasidan-producing strains, and (NH4)2SO4 for the remaining strains. A new variety, A. pullulans var. aubasidani Yurlova, is described for the strains producing aubasidan-like components. The new variety can be distinguished from A. pullulans var. pullulans by the absence of assimilation of methyl-α-D-glucoside and lactose.

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References

  • Andrews JH, Harris RF, Spear RN, Lau GW & Nordheim EV (1994) Morphogenesis and adhesion of Aureobasidium pullulans. Can. J. Microbiol. 40: 6–17

    Google Scholar 

  • Andrews P (1964) Estimation of the molecular weight of proteins by Sephadex gelfiltration. Biochem. J. 91: 222–233

    Google Scholar 

  • Auer DPF & Sevior RJ (1990) Influence of varying nitrogen sources on polysacharide production by Aureobasidium pullulans in batch culture. Appl. Microbiol. Biotechnol. 32: 637–644

    Google Scholar 

  • Bouveng HO, Kiessling H, Lindberg B & McKay J (1962) Polysaccharides elaborated by Pullularia pullulans. The neutral glucan synthesized from sucrose solutions. Acta Chem. Scand. 16: 615–620

    Google Scholar 

  • Bulmer MA, Catley BJ & Kelly PJ (1987) The effect of ammonium ions and pH on the elaboration of the fungi extracellular polysaccharide pullulan, by Aureobasidium pullulans. Appl. Microbiol. Biotechnol. 25: 362–365

    Google Scholar 

  • Catley BJ (1971) Role of pH and nitrogen limitation in the elaboration of the extracellular polysaccharide pullulan by Pullularia pullulans. Appl. Microbiol. 22: 650–654

    Google Scholar 

  • Catley BJ & Whelan WJ (1971) Observations on the structure of pullulan. Arch. Biochem. Biophys. 143: 138–142

    Google Scholar 

  • Catley BJ (1973) The rate of elaboration of the extracellular polysaccharide, pullulan, during growth of Pullularia pullulans. J. Gen. Microbiol. 78: 33–38

    Google Scholar 

  • Deshpande MS, Rale VB & Lynch JM (1992) Aureobasidium pullulans in applied microbiology: a status report. Enzyme Microbiol. Technol. 14: 514–527

    Google Scholar 

  • Elinov NP, Zaikina NA & Vitovskaja GA (1964) The comparative investigation of strains belonging to Aureobasidium pullulans (De Bary) Arnaud. Botanicheskij J. 49: 1192–1199

    Google Scholar 

  • Elinov NP & Matveeva AK (1972) Extracellular glucan produced by Aureobasidium pullulans. Biokhimiya 37: 255–257

    Google Scholar 

  • Elinov NP, Neshataeva EV, Dranishnikov AN & Matveeva AK (1974) Effect of inorganic salts in the synthetic nutrient medium on the structure and properties of glucan formed by Aureobasidium pullulans. Priklad. Biochim. Mikrobiol. 10: 557–562

    Google Scholar 

  • Elinov NP, Marikhin VA, Dranishnikov AN, Myasnikova LP & Maryukhta YB (1975) Peculiarities of the glucan produced by a culture of Aureobasidium (Pullularia) pullulans. Dokl. Akad. Nauk SSSR 221: 213–216

    Google Scholar 

  • Finkelman MAJ & Vardanis A (1982a) Simplified microassay for pullulan synthesis. Appl. Environ. Microbiol. 43: 483–485

    Google Scholar 

  • Finkelman MAJ & Vardanis A (1982b) Pullulan elaboration by Aureobasidium pullulans protoplasts. Appl. Environ. Microbiol. 44: 121–127

    Google Scholar 

  • Golubev VI & Vdovina NV (1974) Monosaccharide composition of extracellular polysaccharides of several Cryptococcus species. Mikrobiologiya 43: 154–156

    Google Scholar 

  • Gorin PAJ & Spencer JFT (1970) Proton magnetic resonance spectroscopy — an aid in identification and chemotaxonomy of yeasts. Adv. Appl. Microbiol. 13: 841–847

    Google Scholar 

  • Hakomori S (1964) A rapid permitilation of glycolipid and polysaccharide catalyzed by methylsulphinil carbanion in dimethylsulphoxide. J. Biochem. 55: 205–208

    Google Scholar 

  • Hamada N & Tsujisaka Y (1983) The structure of the carbohydrate moiety of an acidic polysaccharide produced by Aureobasidium sp. K-1. Agric. Biol. Chem. 47: 1167–1172

    Google Scholar 

  • Hamada N & Yasuto W (1993) Stimulation by ascorbic acid of production of a highly branched β-1,3-glucan by Aureobasidium sp. K-1. Biosci. Biotech. Biochem. 57: 1348–1349

    Google Scholar 

  • Hoog GS de & Yurlova NA (1994) Conidiogenesis, nutritional physiology and taxonomy of Aureobasidium and Hormonema. Antonie van Leeuwenhoek 65: 41–54

    Google Scholar 

  • Imshenetskii AA, Kondrateva TF & Smutko AN (1981) Influence of carbon and nitrogen sources on pullulan biosynthesis by polyploid strains of Pullularia pullulans. Mikrobiologiya 50: 73–76

    Google Scholar 

  • Khvan AV & Kravchenko SB (1985) The connection of physicochemical peculiarities of aubasidan with changing some technological parameters by cultivation. Abstr., Vsesoy. Konfer. Persp. Sozd. Lekarstv. Ispolzov. Biotechnol., Moskva p. 131

  • Leal-Serrano G, Ruperez P & Leal JA (1980) Acidic polysaccharide from Aureobasldium pullulans. Trans. Br. Mycol. Soc. 75: 57–62

    Google Scholar 

  • Leathers TD, Nofsinger GW, Kurtzman CP & Bothast RI (1988) Pullulan production by color variants of Aureobasidium pullulans. J. Indust. Microbiol. 3: 231–239

    Google Scholar 

  • Seviour RJ, Kristiansen B & Harvey L (1984) Morphology of Aureobasidium pullulans during polysaccharide elaboration. Trans. Br. Mycol. Soc. 82: 350–356

    Google Scholar 

  • Seviour RJ, Stasinopoulos SJ, Auer DPF & Gibbs (1992) Production of pullulan and other exopolysaccharides by filamentous fungi. Crit. Rev. Biotech. 12: 279–298

    Google Scholar 

  • Simon L, Bouchet B, Caye-Vaugien C & Gallant DJ (1995) Pullulan elaboration and differentiation of the resting forms of Aureobasidium pullulans. Can. J. Microbiol. 40: 35–45

    Google Scholar 

  • Stasinopolous SJ & Seviour RJ (1989) Exopolysaccharide formation by isolates of Cephalosporium and Acremonium. Mycol. Res. 92: 55–60

    Google Scholar 

  • Taguchi R, Kikuchi Y, Sakano Y & Kobayashi T (1973) Structural uniformity of pullulan produced by several strains of Pullularia pullulans. Agric. Biol. Chem. 37: 1583–1588

    Google Scholar 

  • Ueda S, Fijita K, Komatsu K & Nakashima L (1963) Polysaccharides produced by the genus Pullularia. Appl. Microbiol. 11: 211–215

    Google Scholar 

  • Walt JP van der & Yarrow D (1984) Methods for the isolation, maintenance, classification and identification of yeasts. In: Kreger-van Rij NJW (Ed) The Yeasts, a Taxonomic Study. 4th ed. (pp 45–104). Elsevier, Amsterdam

    Google Scholar 

  • Weijman ACM (1979) Carbohydrate composition of Geotrichum, Trichosporon and allied genera. Antonie van Leeuwenhoek 45: 119–127

    Google Scholar 

  • Weijman ACM & Hoog GS de (1985) Carbohydrate patterns and taxonomy of Sporothrix and Blastobotrys. Antonie van Leeuwenhoek 51: 111–120

    Google Scholar 

  • Weijman ACM & Golubev WI (1987) Carbohydrate patterns and taxonomy of yeasts and yeast-like fungi. In: Hoog GS de, Smith MTh & Weijman ACM (Eds) The Expanding Realm of yeast-like fungi (pp 361–371). Elsevier, Amsterdam

    Google Scholar 

  • Yurlova NA, Gun ZB, Sinitskaja IA, Kashkina MA & Khmara IF (1993) Exoglucan formed by Aureobasidium pullulans (de By.) Arnaud, 1910, F-371. Mikol. Fitopatol. 27: 41–47

    Google Scholar 

  • Yurlova NA, Mokrousov IV & Hoog GS de (1995) Intraspecific variability and exopolysaccharide production in Aureobasidium. Antonie van Leeuwenhoek 68: 57–63

    Google Scholar 

  • Zacharova IL & Kosenko LV (1982) Methods of studying of microbial polysaccharides. Kiev, 192 pp.

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Authors and Affiliations

  1. Department of Microbiology, Chemical-Pharmaceutical Institute, 14, Prof. Popov Str., 197376, St. Petersburg, Russia

    N.A. Yurlova

  2. Centraalbureau voor Schimmelcultures, P.O. Box 273, 3740 Ag, Baarn, the Netherlands

    G.S. de Hoog

Authors
  1. N.A. Yurlova
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  2. G.S. de Hoog
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Yurlova, N., de Hoog, G. A new variety of Aureobasidium pullulans characterized by exopolysaccharide structure, nutritional physiology and molecular features. Antonie Van Leeuwenhoek 72, 141–147 (1997). https://doi.org/10.1023/A:1000212003810

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