Abstract
We have investigated the effects of dibutyrylcAMP, forskolin, carbamylcholine chloride (carbachol),ionomycin, and 12-O-tetradecanoylphorbol-13-acetate(TPA) on the expression of guinea pig pepsinogen mRNA in monolayer cultured gastric chief cells.After exposure of the cells to each of these compoundsfor 4 to 24 hr, and at 48 hr after primary culture,total cellular RNA was isolated using acidguanidium-phenol-chloroform and then was reverse transcribed to cDNA.Obtained cDNA was amplified by polymerase chain reaction(PCR) using primers detecting guinea pig pepsinogen mRNAand human β-actin mRNA as an internal standard. The PCR products were separated and quantifiedusing capillary electrophoresis. Dibutyryl cAMP andforskolin significantly increased pepsinogen mRNA, butcarbachol, ionomycin, and TPA failed to increase that. These findings suggested that pepsinogengene expression was up-regulated by intracellular cAMP,but not by intracellular calcium or protein kinase C inguinea pig chief cells.
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Okayama, N., Itoh, M., Joh, T. et al. Regulatory Mechanism of Pepsinogen Gene Expression in Monolayer Cultured Guinea Pig Gastric Chief Cells. Dig Dis Sci 43, 2744–2749 (1998). https://doi.org/10.1023/A:1026623932350
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DOI: https://doi.org/10.1023/A:1026623932350