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A routine method of DNA-extraction from extremely small metazoans, e.g. single rotifer specimens for RAPD-PCR analyses

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Abstract

A simple and efficient method has been developed for isolating genomic DNA from single specimens of extremely small metazoan animals, e.g. rotifers (Rotifera: Monogononta). The amount and quality of the extracted DNA allows the Random Amplified Polymorphic DNA Polymerase Chain Reaction (RAPD-PCR) to be employed with up to three random primers per specimen. Reproducibility of RAPD-fingerprints has been checked and verified. Exemplarily, RAPD patterns of three Brachionus species (B. angularis, B. calyciflorus, B. urceolaris) amplified with the primer OPA 07 are demonstrated.

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  1. Spezielle Zoologie, University of Osnabrück, D-49069, Osnabrück, Germany

    Christine Leutbecher

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  1. Christine Leutbecher
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Leutbecher, C. A routine method of DNA-extraction from extremely small metazoans, e.g. single rotifer specimens for RAPD-PCR analyses. Hydrobiologia 437, 133–137 (2000). https://doi.org/10.1023/A:1026546705582

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