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Journal of Analytical Chemistry

, Volume 58, Issue 8, pp 731–739 | Cite as

A New Anionic Micellar Mobile Phase System for the Normal-Phase Thin-Layer Chromatography of Amino Compounds: Simultaneous Separation of Aminophenol Isomers

  • A. Mohammad
  • Y. H. Sirwal
Article

Abstract

The chromatography of some amino compounds was performed on high-performance thin-layer plates (silica-gel 60 F254, catalog no. 1.05554, Merck, Germany) using aqueous and alcoholic (MeOH) and aqueous–organic solutions of cationic, anionic, and nonionic surfactants. The results obtained with 0.01 M aqueous sodium dodecyl sulfate (SDS) have been compared with distilled water (i.e., zero SDS) as the eluent. The effects of the surfactant concentration, the nature of the alcohol, and the presence of inorganic salts (NaCl, LiCl, CaCl2, and CoCl2) in the mobile phase were examined in order to understand the mobility pattern of amino compounds. Among the added salts, CaCl2 was found to be the most effective for facilitating an analytically important separation of coexisting ortho-, meta-, and para-aminophenols. The TLC system consisting of a precoated high-performance silica layer as the stationary phase and 0.01 M methanolic SDS plus 0.1 M CaCl2 in a 3 : 7 ratio as the mobile phase was identified as the most suitable system for the separation of o-, m-, and p-APHS from their mixtures. The influence of various impurities, such as amines, phenols, and inorganic cations, on the mobility and the separation of coexisting aminophenol isomers has also been examined. The lower limits of detection of aminophenols were determined on HPTLC plates using I2 vapors as the detection reagent.

Keywords

Surfactant Sodium Dodecyl Sulfate Sodium Dodecyl Sulfate LiCl Nonionic Surfactant 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© MAIK “Nauka/Interperiodica” 2003

Authors and Affiliations

  • A. Mohammad
    • 1
  • Y. H. Sirwal
    • 1
  1. 1.Analytical Laboratory, Department of Applied Chemistry, Faculty of Engineering and TechnologyAligarh Muslim UniversityAligarhIndia

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