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Activity of Lectin-Like Proteins of the Cell Walls and the Outer Organelle Membranes as Related to Endogenous Ligands in Cold-Adapted Seedlings of Winter Wheat

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Abstract

The hemagglutinating activity (HA) of lectin-like components in the cell walls and the outer organelle membranes was studied in freezing-tolerant winter wheat (Triticum aestivum L., cv. Mironovskaya 808) plants in the course of hardening at 2°C, in parallel with the effects of endogenous ligands from the soluble fraction on HA. Low hardening temperature divergently changed HA of the lectin-like components in the cell walls, the outer membranes of nuclei, plastids, and mitochondria, and the microsomal membranes: HA increased in the cell walls, nuclei, and plastids and decreased in the mitochondria and microsomal membranes. Under hardening conditions, with plant growth slowed down, HA of the lectin-like proteins from the outer organelle membranes was inhibited in the presence of the soluble fraction components (soluble ligands); such inhibition was not observed in the case of actively growing nonhardened seedlings. The authors put forward a hypothesis that the lectin-like proteins from both peripheral (cell walls) and intracellular (outer organelle membranes) compartments are essential for developing freezing tolerance. HA of the cell walls and the outer membranes of nuclei and plastids enhanced by hardening manifested positive correlation with freezing tolerance and negative correlation with the growth rate. In contrast, HA of the outer membranes of mitochondria and microsomes was positively related to plant growth and negatively, to freezing tolerance. As negative and positive effectors of membrane-dependent processes, the lectin-like components of the outer organelle membranes seem to control membrane functional activities in the course of cold adaptation.

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REFERENCES

  1. Guy, C.L., Cold Acclimation and Freezing Stress Tolerance: Role of Protein Metabolism, Annu. Rev. Plant Physiol., 1990, vol. 41, pp. 187–223.

    Google Scholar 

  2. Griffith, M. and Antikainen, M., Extracellular Ice Formation in Freezing Tolerance, Adv. Low-Temp. Biol., 1996, vol. 3, pp. 107–139.

    Google Scholar 

  3. Camue, B.P.A., Braeaert, W.F., Kellens, S.T.C., Raikhel, N.V., and Peumans, W.J., Stress-Induced Accumulation of Wheat Germ Agglutinin and Abscisic Acid in Roots of Wheat Seedlings, Plant Physiol., 1989, vol. 91, pp. 1432–1435.

    Google Scholar 

  4. Lyubimova, N.I. and Sal'nikova, E.G., Lectin-Carbohydrate Interaction in the Interrelations between Plant and Pathogen, Prikl. Biokhim. Mikrobiol., 1988, vol. 24, pp. 596–606.

    Google Scholar 

  5. Shakirova, F.M., Bezrukov, M.V., Khairulin, R.M., and Yamaleev, A.M., Increase in the Lectin Activity of Wheat Seedlings under Salt Stress, Izv. Ross. Akad. Nauk, Ser. Biol., 1993, no. 1, pp. 143–145.

    Google Scholar 

  6. Komarova, E.N., Vyskrebentseva, E.I., and Trunova, T.I., The Change in the Actin Activity of the Cell Wall of Winter Wheat Seedlings during Frost Hardening, Dokl. Akad. Nauk, 1993, vol. 329, pp. 680–682.

    Google Scholar 

  7. Timofeeva, O.A., Khokhlova, L.P., Tanenkova, U.I., Olinevich, O.V., and Demakhin, I.F., Lectin Activity as Related to the System of Secondary Messengers during Plant Acclimation to Low Temperatures, Dokl. Akad. Nauk, 1996, vol. 350, pp. 716–718.

    Google Scholar 

  8. Bowles, D.J. and Kauss, H., Carbohydrate-Binding Proteins from Cellular Membranes of Plant Tissues, Plant Sci. Lett., 1975, vol. 4, pp. 411–418.

    Google Scholar 

  9. Aleksidze, G.Ya. and Vyskrebentseva, E.I., Subcellular Location of Lectins in the Roots of Sugar Beet of Different Ages, Fiziol. Rast. (Moscow), 1986, vol. 33, pp. 213–220 (Sov. Plant Physiol., Engl. Transl.).

    Google Scholar 

  10. Vyskrebentseva, E.I. and Borisova, N.N., Distribution of Lectin Activity in Mitochondria of Sugar Beet Roots: Lectin Activity in Mitochondrial Membranes and Matrix, Fiziol. Rast. (Moscow), 1996, vol. 43, pp. 527–532 (Russ. J. Plant Physiol., Engl. Transl.).

    Google Scholar 

  11. Trunova, T.I., Metody opredeleniya morozostoikosti rastenii (Methods for Determination of Plant Frost Resistance), Moscow: Nauka, 1967.

    Google Scholar 

  12. Aleksidze, G.Ya., Korolev, N.P., Semenov, I.L., and Vyskrebentseva, E.I., Lectin Isolation and Their Putative Receptors on the Sugar Beet Root, Fiziol. Rast. (Moscow), 1983, vol. 30, pp. 1069–1076 (Sov. Plant Physiol., Engl. Transl.).

    Google Scholar 

  13. Lutsik, A.V., Metody issledovaniya uglevodnoi spetsi-fichnosti lektinov (metodicheskie ukazaniya) (The Methods for the Study of Lectin Carbohydrate Specificity: Methodological Recommendations), Lvov: Vysshaya Shkola, 1983.

    Google Scholar 

  14. Bradford, M.M., A Rapid and Sensitive Method for Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye Binding, Anal. Biochem., 1976, vol. 8, pp. 248–254.

    Google Scholar 

  15. Markov, U.Yu. and Khavkin, E.E., Plant Lectins: Supposed Functions, Fiziol. Rast. (Moscow), 1983, vol. 30, pp. 852–867 (Sov. Plant Physiol., Engl. Transl.).

    Google Scholar 

  16. Hankins, C.N., Kindinger, J.I., and Shannon, L.M., Legume Galactosidases which Have Hemagglutinin Properties, Plant Physiol., 1980, vol. 65, pp. 618–622.

    Google Scholar 

  17. Lakhtin, V.M., Lectins as Metabolism Regulators, Biotechnologiya, 1986, vol. 6, pp. 66–79.

    Google Scholar 

  18. Korolev, N.P., Aleksidze, G.Ya., Vyskrebentseva, E.I., and Ivanov, I.I., Reconstruction of Endogenous Lectins and Its Receptors in the Bilayer, Dokl. Akad. Nauk SSSR, 1985, vol. 281, pp. 243–246.

    Google Scholar 

  19. Aleksidze, G.Ya., Pipiya, G.G., Gaidamashvili, M.V., and Sanadze, G.A., Lectin Systems of Chloroplasts and Etioplasts in Triticale Leaves, Soobshch. Akad. Nauk Gruzii, 1991, vol. 14, pp. 589–591.

    Google Scholar 

  20. Fal'kovich, T.N., Pronina, N.A., and Semenenko, V.E., Lectin Properties Exhibited by Polypeptides from the Photosystem I Light-Harvesting Complex of Dunaliella salina IPRAS D-209, Fiziol. Rast. (Moscow), 1994, vol. 41, pp. 184–189 (Russ. J. Plant Physiol., Engl. Transl.).

    Google Scholar 

  21. Vyskrebentseva, E.I., Shugaev, A.G., and Aleksidze, G.Ya., The Effect of Cytoplasmic Glycopeptide on the Functional Activity of Mitochondria from Sugar Beet Roots, Fiziol. Rast. (Moscow), 1990, vol. 37, pp. 883–889 (Sov. Plant Physiol., Engl. Transl.).

    Google Scholar 

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Authors and Affiliations

  1. Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, Botanicheskaya ul. 35, Moscow, 127276, Russia

    E. N. Komarova, E. I. Vyskrebentseva & T. I. Trunova

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  1. E. N. Komarova
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  2. E. I. Vyskrebentseva
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  3. T. I. Trunova
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Komarova, E.N., Vyskrebentseva, E.I. & Trunova, T.I. Activity of Lectin-Like Proteins of the Cell Walls and the Outer Organelle Membranes as Related to Endogenous Ligands in Cold-Adapted Seedlings of Winter Wheat. Russian Journal of Plant Physiology 50, 455–460 (2003). https://doi.org/10.1023/A:1024708404315

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