Plant Molecular Biology

, Volume 52, Issue 2, pp 247–258 | Cite as

Transgene integration, organization and interaction in plants

  • Ajay Kohli
  • Richard M. Twyman
  • Rita Abranches
  • Eva Wegel
  • Eva Stoger
  • Paul Christou
Article

Abstract

It has been appreciated for many years that the structure of a transgene locus can have a major influence on the level and stability of transgene expression. Until recently, however, it has been common practice to discard plant lines with poor or unstable expression levels in favor of those with practical uses. In the last few years, an increasing number of experiments have been carried out with the primary aim of characterizing transgene loci and studying the fundamental links between locus structure and expression. Cereals have been at the forefront of this research because molecular, genetic and cytogenetic analysis can be carried out in parallel to examine transgene loci in detail. This review discusses what is known about the structure and organization of transgene loci in cereals, both at the molecular and cytogenetic levels. In the latter case, important links are beginning to be revealed between higher order locus organization, nuclear architecture, chromatin structure and transgene expression.

cereal transformation clean DNA fluorescent in situ hybridization (FISH) transgene integration transgene organization 

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Copyright information

© Kluwer Academic Publishers 2003

Authors and Affiliations

  • Ajay Kohli
    • 1
  • Richard M. Twyman
    • 2
  • Rita Abranches
    • 3
  • Eva Wegel
    • 3
  • Eva Stoger
    • 4
  • Paul Christou
    • 5
  1. 1.Rexagen Corporation, Genome Centre, Norwich Bio-IncubatorNorwichUnited Kingdom
  2. 2.Department of BiologyUniversity of York, HeslingtonYorkUnited Kingdom
  3. 3.Department of Cell and Developmental BiologyJohn Innes CentreNorwichUnited Kingdom
  4. 4.Biologie VII, RWTH AachenAachenGermany
  5. 5.Fraunhofer Institute for Molecular Biology and Applied EcologySchmallenbergGermany

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