Journal of Assisted Reproduction and Genetics

, Volume 15, Issue 8, pp 466–468 | Cite as

Human Embryo Culture: Back to Nature

  • Henry J. Leese


Public Health Developmental Biology Human Embryo Embryo Culture Human Embryo Culture 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Chatot CL, Ziomek CA, Bavister BD, Lewis JL, Torres I: An improved culture medium supports development of random-bred 1-cell mouse embryos in vitro. J Reprod Fertil 1989;86:679–688Google Scholar
  2. 2.
    Lawitts JA, Biggers JD: Optimization of mouse embryo culture media using simplex methods. J Reprod Fertil 1991;91:543–556Google Scholar
  3. 3.
    Thompson JG, Duganzich D: Analysis of cultures systems for bovine in vitro embryo production reported in abstracts of the Proceedings of the International Embryo Transfer Society (1991–1995). Theriogenology 1997;45:195Google Scholar
  4. 4.
    Leese HJ: Metabolic control during preimplantation mammalian development. Hum Reprod Update 1995;1:63–72Google Scholar
  5. 5.
    Kruip ThAM, den Daas JHG: In vitro produced and cloned embryos: Effects of pregnancy, parturition and offspring. Theriogenology 1997;47:43–52Google Scholar
  6. 6.
    Borland RM, Biggers JD, Lechene CP, Taymor ML: Elemental composition of fluid in the human fallopian tube. J Reprod Fertil 1980;58:479–482Google Scholar
  7. 7.
    Leese HJ: The formation and function of oviduct fluid. J Reprod Fertil 1988;82:843–856Google Scholar
  8. 8.
    Gardner DK, Lane M, Calderon I, Leeton J: Environment of the preimplantation human embryo in vivo: Metabolite analysis of oviduct and uterine fluids and metabolism of cumulus cells. Fertil Steril 1996;65(2):349–353Google Scholar
  9. 9.
    Lopata A, Patullo MJ, Chang A, James B: A method for collecting motile spermatozoa from human semen. Fertil Steril 1976;27:677–684Google Scholar
  10. 10.
    Nichol R, Hunter RHF, Gardner DK, Leese HJ, Cooke GM: Concentrations of energy substrates in oviductal fluid and blood plasma of pigs during the periovulatory period. J Reprod Fertil 1996;699–707Google Scholar
  11. 11.
    Dickens CJ, Maguiness SD, Comer MT, Palmer A, Rutherford AJ, Leese HJ: Human tubal fluid: Formation and composition during vascular perfusion of the fallopian tube. Hum Reprod 1995;10:505–508Google Scholar
  12. 12.
    Whitehead A, Leese HJ, Rutherford AJ: The effect of glucose-free medium on human embryo development and the outcome of IVF. J Br Fertil Soc 1996;1:1; Natl Suppl Hum Reprod 1996;11(2):17AGoogle Scholar
  13. 13.
    Mahadevan MM, Miller MM, Moutos DM: Absence of glucose decreases human fertilization and sperm movement characteristics in vitro. Hum Reprod 1997;12(1):119–123Google Scholar
  14. 14.
    Urner F, Sakkas D: Glucose participates in sperm-oocyte fusion in the mouse. Biol Reprod 1996;55:917–922Google Scholar
  15. 15.
    Conaghan J, Handyside AH, Winston RML, Leese HJ: Effects of pyruvate and glucose on the development of human preimplantation embryos in vitro. J Reprod Fertil 1993;99:87–95Google Scholar
  16. 16.
    Tay JI, Rutherford AJ, Killick SR, Maguiness SD, Partridge RJ, Leese HJ: Human tubal fluid: Production, nutrient composition and response to adreneric agents. Hum Reprod 1997;12:2451–2456Google Scholar
  17. 17.
    Pemble LB, Kaye PL: Whole protein uptake and metabolism by mouse blastocysts. J Reprod Fertil; 1976;78:149–157Google Scholar
  18. 18.
    Fischer B, Bavister BD: Oxygen tension in the oviduct and uterus of rhesus monkeys, hamsters and rabbits. J Reprod Fertil 1993;99:673–679Google Scholar
  19. 19.
    Kane MT, Morgan PM, Coonan C: Peptide growth factors and preimplantation development. Hum Reprod Update 1997;3:137–157Google Scholar
  20. 20.
    Leese HJ, Barton AM: Production of pyruvate by isolated mouse cumulus cells. J Exp Zool 1985;234:231–236Google Scholar
  21. 21.
    Gardner DK, Leese HJ: Concentrations of nutrients in mouse oviduct fluid and their effects on embryo development and metabolism in vitro. J Reprod Fertil 1990;88:361–368Google Scholar
  22. 22.
    Gregory L, Leese HJ: Determinants of oocyte and preimplantation embryo quality: Metabolic requirements and the potential role of cumulus cells. JBFS 1996;1(2); Hum Reprod 11 (Natl Suppl):96–102Google Scholar
  23. 23.
    Gregory L, Wells C, Vine SJ, Davies DE, Walker SM: The role of human ovarian cells in the peri-implantation process and early pregnancy. Contem Rev Obstet Gynaecol 1994;6:195–201Google Scholar
  24. 24.
    Taylor CT, Johnson PM: Complement-binding proteins are strongly expressed by human preimplantation blastocysts and cumulus cells as well as gametes. Mol Hum Reprod 1996;2:52–59Google Scholar

Copyright information

© Plenum Publishing Corporation 1998

Authors and Affiliations

  • Henry J. Leese
    • 1
  1. 1.Department of BiologyUniversity of YorkU.K

Personalised recommendations