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Mg2+/Ca2+-ATPase Activity Is Not Enriched in Synaptic Vesicles Isolated from Rat Cerebral Cortex

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Abstract

Neuronal ATPases comprise a wide variety of enzymes which are not uniformly distributed in different membrane preparations. Since purified vesicle fractions have Mg2+/Ca2+-ATPase, the purpose of the present study was to know whether such enzyme activities have a preferential concentration in a synaptic vesicle fraction in order to be used as markers for these organelles. Resorting to a procedure developed in this Institute, we fractionated the rat cerebral cortex by differential centrifugation following osmotic shock of a crude mitochondrial fraction and separated a purified synaptic vesicle fraction over discontinuous sucrose gradients. Mg2+/Ca2+-ATPase activities and ultrastructural studies of isolated fractions were carried out. It was observed that similar specific activities for Mg2+/Ca2+-ATPases were found in all fractions studied which contain synaptic vesicles and/or membranes. Although the present results confirm the presence of Mg2+ and Ca2+-ATPase activities in synaptic vesicles preparations, they do not favor the contention that Mg2+/Ca2+-ATPase is a good marker for synaptic vesicles.

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Authors and Affiliations

  1. Institute de Biología Celular y Neurociencias “Prof. Eduardo De Robertis” Facultad de Medicina, Universidad de Buenos Aires, Paraguay 2155, 1121-, Buenos Aires, Argentina

    Georgina Rodríguez de Lores Arnaiz & Amanda Pellegrino de Iraldi

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  1. Georgina Rodríguez de Lores Arnaiz
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  2. Amanda Pellegrino de Iraldi
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de Lores Arnaiz, G.R., de Iraldi, A.P. Mg2+/Ca2+-ATPase Activity Is Not Enriched in Synaptic Vesicles Isolated from Rat Cerebral Cortex. Neurochem Res 22, 293–296 (1997). https://doi.org/10.1023/A:1022490822175

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  • DOI: https://doi.org/10.1023/A:1022490822175

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