Biotechnology Letters

, Volume 24, Issue 19, pp 1637–1641 | Cite as

Surface and physico-chemical properties of a glycolipid biosurfactant, mannosylerythritol lipid, from Candidaantarctica

  • Hee-Sik Kim
  • Jong-Woon Jeon
  • Seong-Bin Kim
  • Hee-Mock Oh
  • Tae-Jong Kwon
  • Byung-Dae Yoon
Article

Abstract

Candida sp. SY16 produces a glycolipid-type biosurfactant, mannosylerythritol lipid (MEL-SY16) which, at low concentrations, emulsifies hydrocarbons, vegetable oil, and crude oil. The molecular weight of MEL-SY16 was 634 Da and its hydrophilic lipophilic balance (HLB) was 8.8. It decreased the surface tension to 30 dyne cm−1 at a critical micelle concentration (CMC) of 15.8 μM, and the minimum interfacial tension against kerosene (a mixture of petroleum hydrocarbons) was 0.1 dyne cm−1. MEL-SY16 was stable from pH 4 to pH 10, up to 90 °C for 1 h, and against NaCl up to 100 mM. It was readily degraded by microorganisms in activated sludge within a short time, and exhibited very low toxicity to mouse fibroblast cells. These properties confirm that MEL-SY16 is suitable for a variety of environmental applications.

biosurfactant Candida antarctica mannosylerythritol lipid physico-chemical properties surface activity 

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References

  1. Chan EC, Chang CA, Wu TZ, Lin YL (1998) Effects of recombinant lysostaphin on cytotoxicity and interleukin-8 level in normal human epidermal keratinocytes cell line. Biotechnol. Lett. 20: 1053–1056.Google Scholar
  2. Deml G, Anke T, Oberwinkler F, Giannetti BM, Steglich W (1980) Schizonellin A and B, new glycolipids from Schizonella melanogramma. Phytochemistry 19: 83–87.Google Scholar
  3. Griffin WC (1979) Emulsions. In: Encyclopedia of Chemical Technology, 3rd edn., Vol. 8. New York: Wiley-Interscience, pp. 900–930.Google Scholar
  4. Jung HK, Lee JB, Yim GB, Kim EK (1995) Properties of microbial surfactant S-acid. Kor. J. Biotechnol. Bioeng. 10: 71–77.Google Scholar
  5. Kim HS, Jeon JW, Lee HW, Park YI, Seo WT, Oh HM, Katsuragi T, Tani Y, Yoon BD (2002) Extracellular production of a glycolipid biosurfactant, mannosylerythritol lipid, from Candida antarctica. Biotechnol. Lett. 24: 225–229.Google Scholar
  6. Kim HS, Yoon BD, Choung DH, Oh HM, Katsuragi T, Tani Y (1999) Characterization of a biosurfactant, mannosylerythritol lipid produced from Candida sp. SY16. Appl. Microbiol. Biotechnol. 52: 713–721.Google Scholar
  7. Kim HS, Yoon BD, Lee CH, Suh HH, Oh HM, Katsuragi T, Tani Y (1997) Production and properties of a lipopeptide biosurfactant from Bacillus subtilis C9. J. Ferment. Bioengin. 84: 41–46.Google Scholar
  8. Kitamoto D, Akiba S, Hioki C, Tabuchi T (1990) Extracellular accumulation of mannosylerythritol lipids by a strain of Candida antarctica. Agric. Biol. Chem. 54: 31–36.Google Scholar
  9. Kitamoto D, Yanagishita H, Shinbo T, Nakane T, Kamisawa C, Nakahara T (1993) Surface active properties and antimicrobial activities of mannosylerythritol lipids as biosurfactants produced by Candida antarctica. J. Biotechnol. 29: 91–96.Google Scholar
  10. Porter MR, ed. (1991) Use of surfactant theory. In: Handbook of Surfactants. New York: Blackie, Chapman and Hall, pp. 24–48.Google Scholar
  11. Spoeckner S, Wray V, Nimtz M, Lang S (1999) Glycolipids of the smut fungus Ustilago maydis from cultivation on renewable resources. Appl. Microbiol. Biotechnol. 51: 33–39.Google Scholar
  12. Zhang Y, Miller RM (1992) Enhanced octadecane dispersion and biodegradation by a Pseudomonas rhamnolipid surfactant (biosurfactant). Appl. Environ. Microbiol. 58: 3276–3282.Google Scholar
  13. Zhao X, Wakamatsu Y, Shibahara M, Nomura N, Geltinger C, Nakahara T, Murata T, Yokoyama KK (1999) Mannosylerythritol lipid is a potent inducer of apoptosis and differentiation of mouse melanoma cells in culture. Cancer Res. 59: 482–486.Google Scholar

Copyright information

© Kluwer Academic Publishers 2002

Authors and Affiliations

  • Hee-Sik Kim
    • 1
  • Jong-Woon Jeon
    • 1
  • Seong-Bin Kim
    • 1
  • Hee-Mock Oh
    • 1
  • Tae-Jong Kwon
    • 2
  • Byung-Dae Yoon
    • 1
  1. 1.Laboratory of Environmental BiotechnologyKorea Research Institute of Bioscience and BiotechnologyYusong, TaejonKorea
  2. 2.Department of Microbial EngineeringKon-Kuk UniversitySeoulKorea

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