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Euphytica

, Volume 104, Issue 2, pp 87–94 | Cite as

Non-random gametoclonal variation in rice regenerants from callus subcultured for a prolonged period under high osmotic stress

  • Shinya Yoshida
  • Kazuhiko Watanabe
  • Morihiro Fujino
Article

Abstract

Gametoclonal variations were investigated in rice plants regenerated from anther-derived callus after subculture for two different periods (4–6 and 20 weeks) in medium with two levels (292 and 438 mM) of mannitol. A high osmotic stress by mannitol in subculture medium contributed to the maintenance of high regeneration ability. The frequency of diploid regenerants (R0 plants) increased and that of haploids decreased from callus subcultured for the longer period in medium with the higher concentration of mannitol. R1 regenerant lines displayed variations in all six agronomical traits studied at the frequencies of 63% and 88% after subculture for 4–6 weeks and 20 weeks, respectively. High homozygosity was suggested in most of the R1 lines for these traits by the similar levels of variance to those in the control variety. A principal component analysis showed a skewed distribution of variants towards dwarfness and less panicle weight in the regenerants from callus subcultured for 20 weeks with 438 mM mannitol. Two short culmn variants were shown to be due to single or double recessive mutations. The results suggested that prolonged subculture under high osmotic stress causes non-random selection of homozygous gametoclonal variations in rice anther culture.

anther-derived callus Oryza sativa L. osmotic stress gametoclonal variation subculture period 

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Copyright information

© Kluwer Academic Publishers 1998

Authors and Affiliations

  • Shinya Yoshida
    • 1
  • Kazuhiko Watanabe
    • 1
  • Morihiro Fujino
    • 1
  1. 1.Hyogo Prefectural Institute of Agriculture, KasaiHyogoJapan

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