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Euphytica

, Volume 104, Issue 2, pp 119–125 | Cite as

Genetic diversity among Paspalum spp. as determined by RFLPs

  • Robert L. Jarret
  • Zhaowei W. Liu
  • Robert W. Webster
Article

Abstract

The genus Paspalum is characterized by over 400 species that are indigenous to a wide range of stressful habitats and marginal environments. Fifty-one accessions representing 29 Paspalum species were analyzed for DNA restriction fragment length polymorphisms (RFLPs). Fifteen random genomic probes were used in combination with restriction enzyme EcoRI to detect RFLPs, and data were analyzed phenetically. Hybridization with the 15 selected clones resulted in the detection of 261 RFLPs. Among the 261 restriction fragments scored, 204 (78.2%) were phenetically informative. Extensive RFLP variation was found between the species studied. Species affinities based on RFLP data were found to be in close agreement with previously determined relationships based on both morphological and cytological characteristics.

genetic diversity Paspalum restriction fragment length polymorphisms 

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Copyright information

© Kluwer Academic Publishers 1998

Authors and Affiliations

  • Robert L. Jarret
    • 1
  • Zhaowei W. Liu
    • 2
  • Robert W. Webster
    • 1
  1. 1.Georgia Experiment StationUsda/ars, Plant Genetic ResourcesGriffinU.S.A
  2. 2.Department of Crop and Environmental SciencesVirginia Technological Institute and State UniversityBlacksburgU.S.A

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